| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,303,898 | T→C | 12.2% | intergenic (+71/‑113) | metJ → / → yiiX | DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,303,898 | 0 | T | C | 12.2% | 125.8 / 8.9 | 49 | intergenic (+71/‑113) | metJ/yiiX | DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase |
| Reads supporting (aligned to +/- strand): ref base T (18/25); new base C (3/3); total (21/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
AAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGC > minE/2303832‑2303966 | aaaaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTAt < 1:701422/71‑1 (MQ=255) aaaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTAtt > 1:1728957/1‑71 (MQ=255) cggcgCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAAttt < 1:2723425/70‑1 (MQ=255) cggcgCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTg < 1:1430083/71‑1 (MQ=255) tGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTg < 1:2176156/71‑1 (MQ=255) ggAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAAtt < 1:331990/58‑1 (MQ=255) aTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAgg < 1:1914871/71‑1 (MQ=255) tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGc > 1:475944/1‑71 (MQ=255) cGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTg > 1:3460736/1‑55 (MQ=255) cTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGa < 1:684360/71‑1 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACtt < 1:1659941/60‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGaa > 1:661777/1‑55 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTa < 1:2381663/61‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATa < 1:3063233/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATa < 1:552111/70‑1 (MQ=255) tGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAg > 1:486897/1‑71 (MQ=255) cgcTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTg < 1:1361191/40‑1 (MQ=255) cgcTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATaa < 1:551920/70‑1 (MQ=2) cgcTACGCTTATCAAGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGc < 1:2667106/71‑1 (MQ=255) gcTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATaa < 1:201637/69‑1 (MQ=2) gcTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAgg > 1:1192829/1‑71 (MQ=2) gcTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAgg > 1:2156774/1‑71 (MQ=2) gcTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAgg > 1:1526918/1‑71 (MQ=2) aCGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTAtt > 1:2397392/1‑35 (MQ=255) aCGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCa > 1:417656/1‑44 (MQ=255) cGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa > 1:2152599/1‑71 (MQ=255) gCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCAc < 1:1729040/71‑1 (MQ=255) cTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCAcg < 1:2442162/71‑1 (MQ=255) tATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATa < 1:1503696/58‑1 (MQ=255) aTCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGc > 1:2954192/1‑51 (MQ=255) aTCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCg < 1:1539513/62‑1 (MQ=255) tCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTg > 1:1223942/1‑35 (MQ=255) ggCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:3201526/71‑1 (MQ=255) ccTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCg < 1:3737738/56‑1 (MQ=255) cTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa > 1:3401733/1‑51 (MQ=255) cTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa > 1:3733583/1‑51 (MQ=255) tACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCgg < 1:412488/46‑1 (MQ=255) tACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg > 1:1282061/1‑70 (MQ=255) tACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg > 1:1570548/1‑70 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGt > 1:1149222/1‑37 (MQ=255) gTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATa < 1:1090771/71‑1 (MQ=255) atat‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCAcgcc < 1:2807952/56‑1 (MQ=255) tat‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaaca < 1:1638124/71‑1 (MQ=255) tat‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaaca < 1:83032/71‑1 (MQ=255) tat‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaac > 1:1253341/1‑70 (MQ=255) t‑t‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaac > 1:2957717/1‑71 (MQ=255) t‑t‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaac < 1:2315886/71‑1 (MQ=255) gCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAaaaaagc < 1:2064167/68‑4 (MQ=14) cAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAg > 1:1310132/1‑71 (MQ=14) tttATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGc > 1:1598515/1‑69 (MQ=11) | AAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGC > minE/2303832‑2303966 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |