| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,311,509 | T→C | 15.1% | intergenic (+20/‑47) | hslU → / → menA | molecular chaperone and ATPase component of HslUV protease/1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,311,509 | 0 | T | C | 15.1% | 125.4 / 14.8 | 53 | intergenic (+20/‑47) | hslU/menA | molecular chaperone and ATPase component of HslUV protease/1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (17/28); new base C (6/2); total (23/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCTGGATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTA > minE/2311439‑2311574 | cATCTGGATGCGGTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTtcat < 1:805469/71‑1 (MQ=255) aTCTGGATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTtcatc < 1:224553/71‑1 (MQ=255) gATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTtcatca < 1:3066828/67‑1 (MQ=255) gATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTg < 1:1784148/70‑1 (MQ=255) gATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGt < 1:2686092/71‑1 (MQ=255) tGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGttt < 1:63271/71‑1 (MQ=255) cAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTg > 1:2825287/1‑70 (MQ=255) cAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGa < 1:3560332/71‑1 (MQ=255) cAGATGAAGATATGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATggg > 1:59232/1‑66 (MQ=255) gATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGaaa < 1:2805898/71‑1 (MQ=255) aTGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAg < 1:3277467/71‑1 (MQ=255) gAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAg < 1:3270973/69‑1 (MQ=255) aaGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGc < 1:2919860/70‑1 (MQ=255) aGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGaaa > 1:196118/1‑66 (MQ=255) gATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGcccc < 1:181756/71‑1 (MQ=255) aTCTTAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCa > 1:3466071/1‑71 (MQ=255) aTCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAgg > 1:1391033/1‑66 (MQ=255) tCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAt > 1:568882/1‑71 (MQ=255) cTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGAt > 1:1147588/1‑52 (MQ=255) aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTg < 1:2018413/56‑1 (MQ=255) aGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTg < 1:1679339/56‑1 (MQ=255) gCCGTTTTATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTa > 1:1895459/1‑71 (MQ=255) gTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGaaa > 1:335520/1‑55 (MQ=255) gTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTg > 1:864911/1‑71 (MQ=255) ttttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGaaa < 1:2417407/54‑1 (MQ=255) ttttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGaaa < 1:39275/54‑1 (MQ=255) ttttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGaaa < 1:3926996/54‑1 (MQ=255) ttttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGa < 1:2951851/52‑1 (MQ=255) ttttATCCTATAATCGCGTTCAATCAATTTCACCATTGTTTGATGGGGCTGAAAGGCCCCAttttt < 1:2895614/66‑1 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGa > 1:2143447/1‑41 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGa > 1:3227448/1‑41 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGaaa > 1:2199427/1‑52 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGgcg > 1:3497955/1‑71 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGgc < 1:1683236/70‑1 (MQ=255) ttATCCTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGgcg > 1:3361168/1‑71 (MQ=255) tATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAtt < 1:3376771/60‑1 (MQ=255) tCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTg > 1:3227655/1‑46 (MQ=255) tCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAtt < 1:2479327/58‑1 (MQ=255) ccTATAATCGCGTTCAATCATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGta > 1:862161/1‑71 (MQ=255) cTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtat < 1:2236212/71‑1 (MQ=255) cTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtat > 1:1204373/1‑71 (MQ=255) cTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGta < 1:1580596/70‑1 (MQ=255) aaTCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtattat > 1:3733435/1‑70 (MQ=255) aTCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGa < 1:1887511/71‑1 (MQ=255) aTCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGa < 1:264342/71‑1 (MQ=255) gcgTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACt > 1:924377/1‑70 (MQ=255) ttCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCAt < 1:386155/44‑1 (MQ=255) cAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGaacaa > 1:3488445/1‑71 (MQ=255) aaTCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGaacaa < 1:325881/70‑1 (MQ=255) aaTCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGaacaa < 1:2231868/70‑1 (MQ=255) aTCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAacaaca < 1:2189522/71‑1 (MQ=255) tCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAacaaca > 1:536490/1‑70 (MQ=255) cATTTTCACCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACaaa > 1:1175224/1‑71 (MQ=255) ttttCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGtattat < 1:995156/55‑1 (MQ=255) ttttCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAAtt < 1:1745574/71‑1 (MQ=255) ttCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTa < 1:1293219/70‑1 (MQ=255) tcatcaTTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGt > 1:761408/1‑47 (MQ=255) | CATCTGGATGCGTTGGTGGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCCCATTTTTATTGGCGCGTATTATGACTGAACAACAAATTA > minE/2311439‑2311574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |