| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,316,796 | G→A | 28.0% | L157L (CTG→CTA) | glpX → | fructose 1,6‑bisphosphatase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,316,796 | 0 | G | A | 28.0% | 124.6 / 56.2 | 82 | L157L (CTG→CTA) | glpX | fructose 1,6‑bisphosphatase II |
| Reads supporting (aligned to +/- strand): ref base G (36/23); new base A (12/11); total (48/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01 | |||||||||||
TCTGAACCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTACGC > minE/2316727‑2316865 | tCTGAACCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGa > 1:3436387/1‑71 (MQ=255) cTGAACCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGAc > 1:892967/1‑71 (MQ=255) cTGAACCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGAc > 1:2561043/1‑71 (MQ=255) gAACCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACgg > 1:2211734/1‑71 (MQ=255) gAACCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACgg > 1:1916248/1‑71 (MQ=255) aaCCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGt < 1:2655145/71‑1 (MQ=255) aCCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGt > 1:1628046/1‑70 (MQ=255) cGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGAtt > 1:110345/1‑71 (MQ=255) cGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGAtt > 1:2443667/1‑71 (MQ=255) gCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAAAGATTCTg < 1:3063080/69‑1 (MQ=255) gcTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACTGTAACGATTCTGGCTAAAccac < 1:974154/70‑1 (MQ=255) cTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCt < 1:2286159/59‑1 (MQ=255) cTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacg > 1:2570322/1‑71 (MQ=255) cTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacg > 1:2375150/1‑71 (MQ=255) tGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgc > 1:1277261/1‑71 (MQ=255) tGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgc > 1:1877128/1‑71 (MQ=255) gcgcAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTc < 1:3518826/56‑1 (MQ=255) gcgcAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTc < 1:3093711/56‑1 (MQ=255) gcgcAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTc < 1:2554437/56‑1 (MQ=255) gcgcAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAAccacgc > 1:491338/1‑70 (MQ=255) cAATGTAGCGTCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgccacg < 1:1444172/71‑1 (MQ=255) cAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgccacg < 1:1877771/71‑1 (MQ=255) aaTGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGa < 1:456029/71‑1 (MQ=255) gTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTa < 1:1549208/55‑1 (MQ=255) tAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTg < 1:323880/35‑1 (MQ=255) tAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGcc > 1:2601866/1‑71 (MQ=255) tAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGcc > 1:344055/1‑71 (MQ=255) tAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGcc > 1:1420237/1‑71 (MQ=255) gcggcggcgCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGt > 1:319946/1‑71 (MQ=255) ggcggcgCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTa < 1:3055579/71‑1 (MQ=255) ggcgCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCg > 1:3265691/1‑71 (MQ=255) cgcTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCt > 1:1078392/1‑71 (MQ=255) cgcTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCt < 1:2639276/71‑1 (MQ=255) gcTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgccac < 1:2265059/55‑1 (MQ=255) gcTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTAt < 1:3807715/66‑1 (MQ=255) gcTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCt > 1:1894089/1‑70 (MQ=255) gcTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCt > 1:2265844/1‑70 (MQ=255) gcTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCt > 1:1225925/1‑70 (MQ=255) cTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGa < 1:261243/71‑1 (MQ=255) cTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGa < 1:1637096/71‑1 (MQ=255) cTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCt > 1:1215383/1‑69 (MQ=255) cTCGGCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGa < 1:1334058/71‑1 (MQ=255) tCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGa > 1:780747/1‑70 (MQ=255) tCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGa > 1:3832451/1‑70 (MQ=255) ggCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTaaa > 1:1656290/1‑42 (MQ=255) ggCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGaaa > 1:2788309/1‑70 (MQ=255) ggCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGaaa > 1:2433399/1‑70 (MQ=255) ggCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAAt > 1:2734182/1‑71 (MQ=255) ggCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAAt > 1:1133771/1‑71 (MQ=255) gCAAACCGTTTAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTa < 1:1404140/60‑1 (MQ=255) gCAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATg < 1:38744/71‑1 (MQ=255) cAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAAccacgc < 1:2193029/46‑1 (MQ=255) cAAACCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAAccacgc < 1:2197482/46‑1 (MQ=255) aaaCCGTTGAGCGAACTAACGGTAACGATTCTGGCTaaa > 1:187352/1‑39 (MQ=255) aaaCCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATc > 1:3767131/1‑60 (MQ=255) aaaCCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATc > 1:1884678/1‑60 (MQ=255) aaCCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgccac > 1:1166062/1‑47 (MQ=255) aaCCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgccac > 1:3239719/1‑47 (MQ=255) aaCCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATg > 1:1505109/1‑68 (MQ=255) aaCCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATg > 1:1756934/1‑68 (MQ=255) aCCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATgcagc < 1:2594621/71‑1 (MQ=255) aCCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATgcagc < 1:3658272/71‑1 (MQ=255) aCCGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTa > 1:59736/1‑56 (MQ=255) ccGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATgcagca < 1:2073655/71‑1 (MQ=255) ccGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCAAGCCACGATGCCGTTATCGCTGAAATgcagc > 1:3214498/1‑70 (MQ=255) ctttGAGCGAACTGACGGTAACGATTCTGGCTAAAccacgccac < 1:1825643/42‑1 (MQ=255) cGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCaa < 1:1914549/71‑1 (MQ=255) cGTTGAGCGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGcc < 1:1747793/50‑1 (MQ=255) gTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAAc < 1:87219/71‑1 (MQ=255) gTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAAc > 1:3809165/1‑71 (MQ=255) tGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTc > 1:2099641/1‑71 (MQ=255) aGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCg < 1:747231/46‑1 (MQ=255) aGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCg < 1:2353133/46‑1 (MQ=255) cGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCgg > 1:3910196/1‑69 (MQ=255) cGAACTAACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTc > 1:193206/1‑67 (MQ=255) aaCTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGt < 1:536281/70‑1 (MQ=255) aaCTGACGGTAACGATTCTGGCTAAACCACGCCACAATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTa > 1:410771/1‑71 (MQ=255) aCTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTAc < 1:2849166/71‑1 (MQ=255) aCTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTAACGCTGAAATGCAGCAACTCg > 1:3499733/1‑65 (MQ=255) aCTAACGGTAACGATTCTGGCTAAACCACGCCACGATg < 1:1494978/38‑1 (MQ=255) cTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTAcg > 1:2074378/1‑71 (MQ=255) cTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTAcg > 1:2693778/1‑71 (MQ=255) tGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTAcgc > 1:151240/1‑71 (MQ=255) | TCTGAACCTGCCGCTGGCGGATAACCTGCGCAATGTAGCGGCGGCGCTCGGCAAACCGTTGAGCGAACTGACGGTAACGATTCTGGCTAAACCACGCCACGATGCCGTTATCGCTGAAATGCAGCAACTCGGCGTACGC > minE/2316727‑2316865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |