| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,329,107 | T→C | 19.6% | intergenic (‑24/+125) | yiiM ← / ← kdgT | conserved hypothetical protein/2‑keto‑3‑deoxy‑D‑gluconate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,329,107 | 0 | T | C | 19.6% | 103.3 / 20.4 | 51 | intergenic (‑24/+125) | yiiM/kdgT | conserved hypothetical protein/2‑keto‑3‑deoxy‑D‑gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base T (21/20); new base C (2/8); total (23/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
AAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACTC > minE/2329041‑2329175 | aaGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTccc > 1:3637418/1‑70 (MQ=255) ccTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCAtt < 1:3687289/71‑1 (MQ=255) cTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACCAGAAAttt < 1:3946676/63‑1 (MQ=255) aaTCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGAc > 1:1434257/1‑71 (MQ=255) gCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCat < 1:2512501/68‑1 (MQ=255) gCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTa > 1:260438/1‑62 (MQ=255) ccTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCAtattt < 1:3384385/71‑1 (MQ=255) cTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCAtattta < 1:2804273/71‑1 (MQ=255) gtgtATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTccc < 1:631761/53‑1 (MQ=255) tgtATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATatttattt < 1:1924296/71‑1 (MQ=255) tgtATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATatttattt < 1:3513773/71‑1 (MQ=255) tgtATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATatttattt < 1:2849653/71‑1 (MQ=255) tgtATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATatttattt < 1:936263/71‑1 (MQ=255) gtATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATatttattt > 1:1302484/1‑70 (MQ=255) ataCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTccc > 1:1783400/1‑48 (MQ=255) taCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATAtttattttat < 1:1627835/70‑1 (MQ=255) taCATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATg > 1:3679198/1‑71 (MQ=255) cATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGt < 1:3151320/70‑1 (MQ=255) cATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGt < 1:3015439/70‑1 (MQ=255) aTCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGt > 1:1482082/1‑69 (MQ=255) aTCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTc > 1:2302332/1‑70 (MQ=255) aaCCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGc > 1:3049056/1‑71 (MQ=255) ccGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCAtatt < 1:2163599/54‑1 (MQ=255) ccGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCAtatt < 1:3498402/54‑1 (MQ=255) ccGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCAtatt < 1:997621/54‑1 (MQ=255) ggATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGc < 1:2533819/71‑1 (MQ=255) atatCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTg > 1:518141/1‑71 (MQ=255) tatCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGt > 1:3114685/1‑71 (MQ=255) tatCGCATCTGATCCCTCTCCACGAGAAATTTCCCCATTAGACCAtatt < 1:1205477/49‑1 (MQ=255) tCGCATCTGTTCCCTCTCCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGtt < 1:943311/70‑1 (MQ=255) gCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTc < 1:1729129/71‑1 (MQ=255) aTCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTcgc > 1:3896046/1‑71 (MQ=255) tCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTcgcg > 1:2531009/1‑71 (MQ=255) cTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATAtttattttat < 1:2865118/49‑1 (MQ=255) tGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGGGTGCTGTTTTcgcg > 1:1861221/1‑69 (MQ=255) tGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCgg > 1:3933049/1‑70 (MQ=255) tCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCgg > 1:1707359/1‑67 (MQ=255) tCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAg > 1:2808028/1‑71 (MQ=255) cccTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTc < 1:3410916/47‑1 (MQ=255) cccTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCa > 1:1218090/1‑48 (MQ=255) ccTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCCGGc < 1:2936218/50‑1 (MQ=255) ctcCACGAGAAATTTCCCCATTAGACCAtatttat > 1:2363141/1‑35 (MQ=255) ctcCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATc > 1:503038/1‑70 (MQ=255) tcCACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATc < 1:342920/69‑1 (MQ=255) ccACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTcg < 1:2791751/57‑1 (MQ=255) cACGAGAAATTTCCCCATTAGACCATAtttatttta > 1:2988843/1‑36 (MQ=255) cACGAGAAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcag < 1:2321988/70‑1 (MQ=255) gagaAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcaggcag > 1:1449888/1‑71 (MQ=255) gagaAATTCCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcaggcag > 1:82456/1‑71 (MQ=255) gaAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTAt < 1:3390562/61‑1 (MQ=255) gaAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTAt < 1:769391/61‑1 (MQ=255) gaAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcaggcagg > 1:2427541/1‑70 (MQ=255) aTTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACt < 1:994103/71‑1 (MQ=255) tttCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACTc < 1:1628931/71‑1 (MQ=255) ttCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcaggcagg > 1:3600548/1‑65 (MQ=255) ttCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcaggcagg > 1:80125/1‑65 (MQ=255) ttCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCcaggcagg > 1:2082868/1‑65 (MQ=255) | AAGCCTGAATCTTGCCTGTGTATACATCAACCGGATATCGCATCTGTTCCCTCTCCACGAGAAATTTCCCCATTAGACCATATTTATTTTATGTCAGGCGTGCTGTTTTCGCGGTAAGTATCCAGGCAGGAACTC > minE/2329041‑2329175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |