| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,331,252 | A→C | 61.1% | intergenic (‑157/+273) | sodA ← / ← fdhD | superoxide dismutase, Mn/formate dehydrogenase formation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,331,252 | 0 | A | C | 61.1% | 72.1 / 50.5 | 59 | intergenic (‑157/+273) | sodA/fdhD | superoxide dismutase, Mn/formate dehydrogenase formation protein |
| Reads supporting (aligned to +/- strand): ref base A (9/14); new base C (22/14); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAATGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCGA > minE/2331188‑2331314 | cAATGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACt > 1:1891708/1‑70 (MQ=255) aaTGCTGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTa < 1:1821770/71‑1 (MQ=255) aaTGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACtt < 1:603953/70‑1 (MQ=255) tttCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATg > 1:637332/1‑63 (MQ=255) ttCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCa > 1:1020269/1‑55 (MQ=255) ttCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTaa > 1:2852444/1‑60 (MQ=255) ttCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTg > 1:3720495/1‑71 (MQ=255) tCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAAt < 1:252489/60‑1 (MQ=255) aaGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAg > 1:3907905/1‑70 (MQ=255) aaGGGTATGGTTTTGCAAGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAg > 1:331034/1‑70 (MQ=255) aGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAAt > 1:3249319/1‑55 (MQ=255) gggTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTa > 1:988015/1‑70 (MQ=255) tgTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTaaa < 1:2474186/70‑1 (MQ=255) ggTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGt > 1:2146903/1‑58 (MQ=255) ggTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGt > 1:3443170/1‑58 (MQ=255) ggTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTaaa < 1:22874/71‑1 (MQ=255) ggTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGc > 1:908999/1‑64 (MQ=255) gTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGc < 1:1229334/63‑1 (MQ=255) gTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGc < 1:109212/63‑1 (MQ=255) gTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTaa < 1:2532320/69‑1 (MQ=255) tATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATc > 1:3582258/1‑71 (MQ=255) aTGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAATTc > 1:3932086/1‑70 (MQ=255) tGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGa > 1:467166/1‑71 (MQ=255) ggTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGa > 1:3679141/1‑70 (MQ=255) gTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGAc < 1:515112/70‑1 (MQ=255) ttttGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACaa > 1:3562784/1‑71 (MQ=255) ttttGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACaa < 1:3906902/71‑1 (MQ=255) ttttGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGt < 1:2990256/51‑1 (MQ=255) tttGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTATTGc > 1:2448259/1‑47 (MQ=255) ttGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAAcg > 1:3711050/1‑71 (MQ=255) tGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGAc < 1:1812039/66‑1 (MQ=255) gCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAAcgg < 1:3424222/70‑1 (MQ=255) gCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAAcgg < 1:862565/70‑1 (MQ=255) aGGAAAATGCCCGAGATGTGAAGCAAATCACCCTCTTAATGCCGTGATTGCGAGTAAATCGACAAcg < 1:3469139/67‑1 (MQ=255) ggAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACggcggc < 1:3108632/71‑1 (MQ=255) gAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGa < 1:3287068/60‑1 (MQ=255) gAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGa < 1:2423263/60‑1 (MQ=255) aaaaTGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCaa > 1:3856441/1‑71 (MQ=255) aaaaTGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCaa > 1:3782967/1‑71 (MQ=255) aTGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAg > 1:2839837/1‑71 (MQ=255) aTGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAg < 1:2655560/71‑1 (MQ=255) cccGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAAcggcg > 1:1301340/1‑61 (MQ=255) ccGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCg > 1:1011645/1‑70 (MQ=255) cGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAAt < 1:291661/48‑1 (MQ=255) gagaTGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGaaa < 1:2173629/71‑1 (MQ=255) gagaTGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGa > 1:1660612/1‑69 (MQ=255) gagaTGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGa > 1:2519403/1‑69 (MQ=255) gagaTGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGa > 1:885311/1‑69 (MQ=255) gagaTGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTa > 1:3611225/1‑44 (MQ=255) gagaTGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGaaa < 1:86185/71‑1 (MQ=255) gagaTGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGaaa < 1:2412087/71‑1 (MQ=255) agaTGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCaa > 1:1140024/1‑61 (MQ=255) tgtgAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGtt < 1:2051632/71‑1 (MQ=255) tgAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCg > 1:2616625/1‑43 (MQ=255) tgAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTaa < 1:3516244/71‑1 (MQ=255) aGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCg < 1:2868067/71‑1 (MQ=255) cAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAgg < 1:3789370/62‑1 (MQ=255) cAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAgg < 1:1789007/62‑1 (MQ=255) aaaTCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCGa > 1:2359647/1‑69 (MQ=255) | CAATGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCGA > minE/2331188‑2331314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |