| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,334,140 | A→G | 17.4% | K530R (AAG→AGG) | fdoG → | formate dehydrogenase‑O, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,334,140 | 0 | A | G | 17.4% | 97.5 / 15.1 | 46 | K530R (AAG→AGG) | fdoG | formate dehydrogenase‑O, large subunit |
| Reads supporting (aligned to +/- strand): ref base A (24/14); new base G (2/6); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
TGATGAAGGCCTTCTTTGGTGATAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGTCAATGGCTATAT > minE/2334074‑2334209 | tgatgaAGGCCTTCTTTGGTGATAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAg < 1:581875/68‑1 (MQ=255) gatgaAGGCCTTCTTTGGTGATATAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAg < 1:525671/67‑1 (MQ=255) aGGCCTTCTTTGGTGATAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATa > 1:3020222/1‑69 (MQ=255) aGGCCTTCTTTGGTGATAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATa > 1:2278096/1‑69 (MQ=255) gCCTTCTTTGGTGATAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAgg > 1:3081300/1‑71 (MQ=255) cttcttTGGTGATAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAgg > 1:3451874/1‑69 (MQ=255) cttcttTGGTGATAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGc > 1:3008779/1‑70 (MQ=255) ttGGTGATAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTacgac > 1:602554/1‑71 (MQ=255) ttGGTGATAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTacgac > 1:1340138/1‑71 (MQ=255) aTAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTg > 1:1701796/1‑71 (MQ=255) tAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAGGTGGGATAAAGGCTACGACGTCCTg > 1:1633563/1‑70 (MQ=255) tAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCt > 1:2627777/1‑69 (MQ=255) gACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATa < 1:3571736/47‑1 (MQ=255) gACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATa < 1:318509/47‑1 (MQ=255) cgccggAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATAc > 1:1057142/4‑68 (MQ=255) cggAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCt > 1:3164193/1‑58 (MQ=255) cggAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTc > 1:555328/1‑68 (MQ=255) cggAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTc > 1:1449265/1‑68 (MQ=255) gAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAgat < 1:670814/71‑1 (MQ=255) aaaTAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAgatga > 1:3167172/1‑71 (MQ=255) aaTAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTac > 1:3622375/1‑45 (MQ=255) aaTAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAgatgat > 1:1792938/1‑71 (MQ=255) aTAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATaa > 1:1520643/1‑37 (MQ=255) gCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTc > 1:1481193/1‑59 (MQ=255) tGGGGCTTTGACTGGTTGCCGAGGTGGGATAAAGGCTACGACGTCCTGCAATACt < 1:548878/55‑1 (MQ=255) tGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGaaa > 1:2131928/1‑69 (MQ=255) gggCTTTGACTGGTTGCCGAGGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAaga < 1:1562738/69‑1 (MQ=255) cTTTGACTGGTTGCCGAGGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCa < 1:2113217/71‑1 (MQ=255) cTTTGACTGGTTGCCGAGGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCa < 1:125404/71‑1 (MQ=255) ttGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGt < 1:3210798/37‑1 (MQ=255) tGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAgggaag < 1:1318755/69‑4 (MQ=255) tGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAgg > 1:3409503/1‑65 (MQ=255) gACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGt > 1:1336331/1‑71 (MQ=255) ggTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCg < 1:670888/45‑1 (MQ=255) gTTGCCGAGGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAgatga < 1:2596522/50‑1 (MQ=255) gTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAgg < 1:1102590/59‑1 (MQ=255) gTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGTCAATg > 1:2436109/1‑71 (MQ=255) tGCCGAGGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGTCAATgg > 1:3491262/1‑70 (MQ=255) tGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGTCa < 1:3626595/66‑1 (MQ=255) ccGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCg > 1:792308/1‑40 (MQ=255) ccGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGatat < 1:3944576/44‑1 (MQ=255) cGAGGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGTCAAt < 1:3918658/65‑1 (MQ=255) cGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGTCAATGGCtat < 1:2383926/71‑1 (MQ=255) cGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGTCAATGGCtat < 1:1816337/71‑1 (MQ=255) cGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGTCAATGGCt < 1:66370/69‑1 (MQ=255) gAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGTCAATGGCtata > 1:3502804/1‑71 (MQ=255) aaGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGTCAATGGCTatat > 1:758476/1‑71 (MQ=255) aaGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGTCAATGGCTatat < 1:919219/71‑1 (MQ=255) aGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAgat < 1:820039/40‑1 (MQ=255) | TGATGAAGGCCTTCTTTGGTGATAAAGCGACGGCGGAAAATAGCTGGGGCTTTGACTGGTTGCCGAAGTGGGATAAAGGCTACGACGTCCTGCAATACTTCGAGATGATGAAAGAGGGCAAGGTCAATGGCTATAT > minE/2334074‑2334209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |