| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,371,046 | G→A | 8.9% | intergenic (+42/+339) | fadA → / ← fre | 3‑ketoacyl‑CoA thiolase/flavin reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,371,046 | 0 | G | A | 8.9% | 208.9 / 6.8 | 67 | intergenic (+42/+339) | fadA/fre | 3‑ketoacyl‑CoA thiolase/flavin reductase |
| Reads supporting (aligned to +/- strand): ref base G (32/29); new base A (2/4); total (34/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTATTGCGACGGTGTTTGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCACCAATTGCCGGA > minE/2370976‑2371115 | gTATTGCGACGGTGTTTGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCg < 1:166410/71‑1 (MQ=255) aTTGCGACGGTGTTTGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGAt > 1:2384978/1‑71 (MQ=255) ttGCGACGGTGTTTGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGAt < 1:1291123/70‑1 (MQ=255) ttGCGACGGTGTTTGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATc > 1:342801/1‑71 (MQ=255) ttGCGACGGTGTTTGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATc > 1:1706256/1‑71 (MQ=255) gCGACGGTGTTTGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCg > 1:1077548/1‑71 (MQ=255) ggTGTTTGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAc‑c < 1:1825849/71‑1 (MQ=255) tgtTTGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑Caa < 1:2351709/71‑1 (MQ=255) tgtTTGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑Caa < 1:2347182/71‑1 (MQ=255) tttGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATg < 1:1481760/71‑1 (MQ=255) tGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGt < 1:11067/70‑1 (MQ=255) tGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGt < 1:3644893/70‑1 (MQ=255) gAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAg > 1:2723594/1‑71 (MQ=255) gAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAg > 1:165887/1‑71 (MQ=255) aGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAgg < 1:2719369/71‑1 (MQ=255) aGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAg < 1:3094135/70‑1 (MQ=255) gCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGc > 1:45117/1‑71 (MQ=255) gggTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCt < 1:353922/71‑1 (MQ=255) gTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGAt < 1:2970372/50‑1 (MQ=255) ttAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGAt > 1:912343/1‑70 (MQ=255) ttAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGAt > 1:2739348/1‑70 (MQ=255) tAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTa > 1:1821039/1‑61 (MQ=255) ccACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGAc < 1:3570427/71‑1 (MQ=255) cACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGc < 1:2324633/48‑1 (MQ=255) cACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGc > 1:2232994/1‑60 (MQ=255) aCTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAg < 1:2602251/57‑1 (MQ=255) aCTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGAcgc > 1:3433740/1‑71 (MQ=255) aCTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGAcg > 1:3468599/1‑70 (MQ=255) tGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACgcgc > 1:1605678/1‑70 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGa > 1:186351/1‑63 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAg < 1:661895/71‑1 (MQ=255) cGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGc < 1:147986/71‑1 (MQ=255) gATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGt > 1:3149180/1‑71 (MQ=255) aTGCGGCGTGAACGCCTTATCCAGCCTACCAAACCCGCACTGATTGTAGGCATGATAAGACGCGCAAGCGt < 1:2634653/71‑1 (MQ=2) tGCGGCGTGAACGCCTTGTCCGGCCTACAAAACCGAAAC‑GTATGTAGGCCTGATAAGACGCGTCAGCGTc > 1:2007651/1‑70 (MQ=9) tGCGGCGTGAACGCCTTGTCCGGCCTACAAAACCGAAAC‑GTATGTAGGCCTGATAAGACGCGTCAGCGTCg < 1:2592090/71‑1 (MQ=9) tGCGGCGTGAACGCCTTGTCCGGCCTACAAAACCGAAAC‑GTATGTAGGCCTGATAAGACGCGTCAGCGTCg < 1:3269882/71‑1 (MQ=9) tGCGGCGTGAACGCCTTATCCAGCCTACCAAACCCGCACTGATTGTAGGCATGATAAGACGCGCAAGCGTc > 1:1553180/1‑71 (MQ=2) cggcgTGAACGCCTTGTCCGGCCTACAAAACCGAAAC‑GTATGTAGGCCTGATAAGACGCGTCAGCGTCGCa < 1:878675/71‑1 (MQ=9) ggcgTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGc < 1:3178851/69‑1 (MQ=255) cgTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCAt > 1:3090557/1‑69 (MQ=255) cgTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCAt > 1:2869542/1‑69 (MQ=255) cgTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCAt > 1:2460578/1‑69 (MQ=255) gTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAg > 1:2394320/1‑71 (MQ=255) tGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAg < 1:2461296/50‑1 (MQ=255) tGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGACAGCGTCGCATCAg < 1:576410/70‑1 (MQ=255) aaCGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGca < 1:3238048/71‑1 (MQ=255) aaCGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGca > 1:3861892/1‑71 (MQ=255) cGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAg > 1:3689881/1‑36 (MQ=255) cGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATaa > 1:877349/1‑45 (MQ=255) cGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc > 1:2480595/1‑68 (MQ=255) gCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGcatca < 1:3754189/71‑1 (MQ=255) ccTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGcatcat < 1:1729850/71‑1 (MQ=255) ttATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATg > 1:3785551/1‑70 (MQ=255) tATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCa > 1:612392/1‑71 (MQ=255) tATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCa > 1:2640045/1‑71 (MQ=255) tATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCa > 1:878056/1‑71 (MQ=255) ccGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCAcc > 1:1794831/1‑70 (MQ=255) cGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCACCaa < 1:1384032/71‑1 (MQ=255) gTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCg < 1:2517061/46‑1 (MQ=255) gTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCACCAAtt < 1:3476941/71‑1 (MQ=255) tCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATc > 1:432085/1‑52 (MQ=255) tACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATg < 1:3685932/60‑1 (MQ=255) aCCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCACCAATTg < 1:3366187/68‑1 (MQ=255) ccGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCACCAATTGc < 1:3083887/68‑1 (MQ=255) ccGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCACCAATTGc < 1:1577020/68‑1 (MQ=255) cGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCACCAATTGCCGGa > 1:1352357/1‑71 (MQ=255) cGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCACCAATTGCCGGa > 1:3214809/1‑71 (MQ=255) | GTATTGCGACGGTGTTTGAGCGGGTTTAACCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCAC‑CAATGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCATCATGCACCAATTGCCGGA > minE/2370976‑2371115 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |