| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,401,662 | T→C | 8.5% | K222R (AAA→AGA) | xerC ← | site‑specific tyrosine recombinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,401,662 | 0 | T | C | 8.5% | 258.6 / 12.6 | 94 | K222R (AAA→AGA) | xerC | site‑specific tyrosine recombinase |
| Reads supporting (aligned to +/- strand): ref base T (38/48); new base C (4/4); total (42/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
CGAGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTACCAAA > minE/2401592‑2401732 | cGAGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGtt < 1:1181895/71‑1 (MQ=255) gAGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGttt < 1:590890/71‑1 (MQ=255) gAGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGttt < 1:123110/71‑1 (MQ=255) gTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCt < 1:1215433/71‑1 (MQ=255) gTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCt < 1:952014/71‑1 (MQ=255) tGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTc < 1:758453/69‑1 (MQ=255) tGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTc < 1:3646005/69‑1 (MQ=255) tGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTg > 1:2754664/1‑71 (MQ=255) aCGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGca < 1:3336985/71‑1 (MQ=255) aCGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGca > 1:834341/1‑71 (MQ=255) cGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGca > 1:30647/1‑70 (MQ=255) cGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGca > 1:3406706/1‑70 (MQ=255) cGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGca > 1:3709446/1‑70 (MQ=255) tAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGTCAAAGCGTTTCTGc < 1:141802/67‑1 (MQ=255) tAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGc < 1:120435/67‑1 (MQ=255) tAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACAt < 1:2250100/71‑1 (MQ=255) tAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACAt < 1:1271621/71‑1 (MQ=255) tAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACAt > 1:1263412/1‑71 (MQ=255) aTTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTa < 1:583256/71‑1 (MQ=255) aTTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTa > 1:2274618/1‑71 (MQ=255) aTTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTCCACATTa < 1:3944849/71‑1 (MQ=255) tttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCt < 1:962201/62‑1 (MQ=255) tttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAc > 1:1699001/1‑71 (MQ=255) ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcg > 1:478246/1‑71 (MQ=255) ttATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcg > 1:3314535/1‑71 (MQ=255) tATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcg > 1:2548818/1‑70 (MQ=255) tATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcg < 1:2511685/70‑1 (MQ=255) ggATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcgcgcg > 1:3243339/1‑69 (MQ=255) aTGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTc < 1:431360/52‑1 (MQ=255) tGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGAt < 1:1230371/71‑1 (MQ=255) aaCGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATAc > 1:2802609/1‑71 (MQ=255) aCGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACg < 1:2008093/71‑1 (MQ=255) aCGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACg < 1:1778964/71‑1 (MQ=255) cGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGc < 1:3615256/71‑1 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTc > 1:2416798/1‑46 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCt < 1:1416217/71‑1 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCt > 1:2081537/1‑71 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCt < 1:1411625/71‑1 (MQ=255) gTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCt < 1:1415218/71‑1 (MQ=255) tGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCtt > 1:3938043/1‑71 (MQ=255) attattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATAc < 1:652505/65‑1 (MQ=255) ttattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGttt > 1:79570/1‑41 (MQ=255) ttattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcgcgcg > 1:382704/1‑57 (MQ=255) ttattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcgcgcg > 1:3622360/1‑57 (MQ=255) ttattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcgcgcg > 1:1954101/1‑57 (MQ=255) tattCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGcc > 1:2507119/1‑70 (MQ=255) attCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGccc < 1:1387979/70‑1 (MQ=255) ttCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAg < 1:3535730/71‑1 (MQ=255) ttCAGACCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCa < 1:1104031/70‑1 (MQ=255) gCCCTTGTTTTATGCCCGATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTc < 1:3698493/71‑1 (MQ=255) gCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTc < 1:2674888/71‑1 (MQ=255) gCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTc < 1:1361550/71‑1 (MQ=255) ccTTGTTTTATGCCCCATTCTGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGa < 1:3494043/71‑1 (MQ=255) cTTGTTTTTTGCCCCATTCGGCAAAGCGTCTCTGCTCATTACGCGCGGAGATACGCTTGCCCAGTTTCGa < 1:3498622/70‑1 (MQ=255) ttGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGa > 1:72254/1‑69 (MQ=255) tGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGa > 1:1460411/1‑68 (MQ=255) tGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGa > 1:548173/1‑68 (MQ=255) tGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGa > 1:2409410/1‑68 (MQ=255) gTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAg < 1:2933775/70‑1 (MQ=255) gTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACAt < 1:2776764/36‑1 (MQ=255) gTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAg > 1:63198/1‑70 (MQ=255) ttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCt > 1:3216884/1‑51 (MQ=255) ttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCt > 1:2470430/1‑51 (MQ=255) ttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCt > 1:2052770/1‑51 (MQ=255) ttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaa < 1:2817506/70‑1 (MQ=255) ttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaa > 1:1980214/1‑70 (MQ=255) ttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaa < 1:229332/70‑1 (MQ=255) ttATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGAt > 1:957688/1‑46 (MQ=255) aTGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaaaa < 1:3952686/70‑1 (MQ=255) tGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAg < 1:425150/70‑1 (MQ=255) ccccATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAgcgc < 1:1984254/71‑1 (MQ=255) cccATTCGGCAAAGCGTTTCTGCACATTACGCGCGga > 1:3298146/1‑37 (MQ=255) cccATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGcgcg < 1:1587078/71‑1 (MQ=255) cccATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGcgcg < 1:1922289/71‑1 (MQ=255) cATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc > 1:3939226/1‑71 (MQ=255) cATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgt > 1:3517588/1‑70 (MQ=255) ttCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAgcgc < 1:83120/66‑1 (MQ=255) ttCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcgt > 1:311938/1‑71 (MQ=255) ttCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtcg > 1:3438681/1‑70 (MQ=255) cGGCAAAGCGTTTCTGCACATTACGCGCGGAGATa > 1:780626/1‑35 (MQ=255) cGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCt > 1:1956801/1‑71 (MQ=255) ggCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGa < 1:1429110/55‑1 (MQ=255) ggCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCtt > 1:1558097/1‑71 (MQ=255) ggCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCt < 1:2199791/70‑1 (MQ=255) gCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTc < 1:2816940/71‑1 (MQ=255) gCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTc < 1:2664988/71‑1 (MQ=255) cAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTc < 1:1402445/70‑1 (MQ=255) cAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCg < 1:188144/71‑1 (MQ=255) cAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCg < 1:1481547/71‑1 (MQ=255) aaGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCgtcgtc < 1:487685/65‑1 (MQ=255) aaGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTc > 1:1201771/1‑45 (MQ=255) aGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTa > 1:1124252/1‑71 (MQ=255) gCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTa < 1:880803/70‑1 (MQ=255) cGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTAcc < 1:2796240/71‑1 (MQ=255) gTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGa > 1:1351684/1‑43 (MQ=255) tttCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGa < 1:3130797/46‑1 (MQ=255) tttCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAgc > 1:379727/1‑52 (MQ=255) tttCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTACCaa < 1:493553/71‑1 (MQ=255) ttCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTACCaaa > 1:2985391/1‑71 (MQ=255) ttCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGAGTCGTCTTCGCTACCaa > 1:760864/1‑70 (MQ=255) | CGAGTGACGTAATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGCTACCAAA > minE/2401592‑2401732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |