| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,419,194 | C→T | 9.1% | E273E (GAG→GAA) | wzyE ← | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,419,194 | 0 | C | T | 9.1% | 191.8 / 7.8 | 66 | E273E (GAG→GAA) | wzyE | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Reads supporting (aligned to +/- strand): ref base C (35/25); new base T (4/2); total (39/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCGCGGACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTCACAT > minE/2419124‑2419261 | aaTCGCGGACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATtc < 1:3695645/71‑1 (MQ=255)aaTCGCGGACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATtc < 1:2693787/71‑1 (MQ=255) ggACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCAc > 1:3839652/1‑71 (MQ=255) gACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACg > 1:547457/1‑71 (MQ=255) aCAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACg > 1:472112/1‑70 (MQ=255) aCAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACg > 1:2009103/1‑70 (MQ=255) cAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGc > 1:2556921/1‑71 (MQ=255) aaTTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATtct < 1:334234/62‑1 (MQ=255) ggAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCgag > 1:3156675/1‑69 (MQ=255) gCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGgt < 1:3172485/71‑1 (MQ=255) aGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTc > 1:898086/1‑71 (MQ=255) ggCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTccc > 1:2553226/1‑54 (MQ=255) gCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTcgc < 1:1580691/71‑1 (MQ=255) gCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTcgc > 1:2009199/1‑71 (MQ=255) gCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTcgc < 1:3449674/71‑1 (MQ=255) cTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAg > 1:352242/1‑61 (MQ=255) cTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAg > 1:1163538/1‑71 (MQ=255) aGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTcgc < 1:1986602/63‑1 (MQ=255) gTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCgag < 1:2595752/52‑1 (MQ=255) tCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa < 1:2858406/71‑1 (MQ=255) tCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTgaga > 1:1460024/1‑69 (MQ=255) gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTTTCCCACGGCgaga > 1:3383449/1‑50 (MQ=255) gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCgaga < 1:3109236/50‑1 (MQ=255) gATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa > 1:1312776/1‑69 (MQ=255) tgttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATagat > 1:1891640/1‑69 (MQ=255) tgttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATagag > 1:2183249/1‑70 (MQ=255) tgttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATagag > 1:3866120/1‑70 (MQ=255) gttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATa < 1:2916502/66‑1 (MQ=255) ttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGaaa < 1:886322/71‑1 (MQ=255) ttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGaa < 1:3838462/70‑1 (MQ=255) ttgtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGaa < 1:2588538/70‑1 (MQ=255) gtCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACgg > 1:1376917/1‑39 (MQ=255) tCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACg > 1:1052483/1‑70 (MQ=255) gTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTAGCGAGTGAGATaga > 1:1488441/1‑62 (MQ=255) aGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATaga > 1:1164571/1‑60 (MQ=255) gTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAgaga < 1:3084963/61‑1 (MQ=255) gTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAg < 1:3184896/70‑1 (MQ=255) gTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAg > 1:2254335/1‑70 (MQ=255) ttCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGgtgt < 1:2232859/43‑1 (MQ=255) tCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGaaa > 1:474380/1‑61 (MQ=255) cTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGgtgt < 1:2543902/41‑1 (MQ=255) cTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAAc < 1:1118108/70‑1 (MQ=255) cTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg > 1:2130602/1‑71 (MQ=255) cTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg > 1:492235/1‑71 (MQ=255) cTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg > 1:94378/1‑71 (MQ=255) cAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGta < 1:3836153/61‑1 (MQ=255) cAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGta > 1:1050380/1‑61 (MQ=255) cAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACg < 1:2442703/68‑1 (MQ=255) cAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCtt > 1:1038969/1‑71 (MQ=255) aCAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGtat > 1:293822/1‑60 (MQ=255) cAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCAt < 1:1775859/71‑1 (MQ=255) cAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCCCGAGTGAGATAGAGAAACGTATAGAACGCTTCAt < 1:2011107/71‑1 (MQ=255) aGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATc < 1:2494682/71‑1 (MQ=255) cAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgc > 1:963340/1‑71 (MQ=255) aaCGCCAGATTCTCCCACGGCGATAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgcc < 1:2072814/71‑1 (MQ=255) aCGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgccg > 1:2603502/1‑71 (MQ=255) aCGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATcgccg < 1:591803/71‑1 (MQ=255) gCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCa > 1:2853132/1‑63 (MQ=255) ccAGATTTTCCCACGGCGAGAAGGTGTCGCGAGTg > 1:975478/1‑35 (MQ=255) ccAGATTTTCCCACGGCGAGAAGGTGTCGAGAGTg > 1:2275016/1‑35 (MQ=37) ccAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCa < 1:3695965/62‑1 (MQ=255) cAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTca < 1:3377227/71‑1 (MQ=255) aGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTcac < 1:866637/71‑1 (MQ=255) gATTTTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTcaca < 1:3951470/71‑1 (MQ=255) gATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTc > 1:2137652/1‑58 (MQ=255) gATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTcaca > 1:11021/1‑71 (MQ=255) aTTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTCAcat > 1:1772434/1‑71 (MQ=255) ttCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAgaga > 1:3920318/1‑39 (MQ=255) ttCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAgaga > 1:3372326/1‑39 (MQ=255) | AATCGCGGACAATTGGAGCCAGGCCCTGGAAGTCGATGTTGTCGTAGTTCTGCAACAGCAACGCCAGATTCTCCCACGGCGAGAAGGTGTCGCGAGTGAGATAGAGAAACGTATAGAACGCTTCATCGCCGCTCACAT > minE/2419124‑2419261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |