| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,425,537 | G→A | 12.8% | T185T (ACC→ACT) | rffG ← | dTDP‑glucose 4,6‑dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,425,537 | 0 | G | A | 12.8% | 225.6 / 19.7 | 86 | T185T (ACC→ACT) | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (45/30); new base A (7/4); total (52/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
GCGCGTTGAGGATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTCGCTGCTGGCTTTTGAC > minE/2425467‑2425606 | gcgcGTTGAGGATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTg > 1:2546474/1‑71 (MQ=255)gcgcGTTGAGGATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTg > 1:447245/1‑71 (MQ=255) cgcgTTGAGGATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTgg < 1:794601/71‑1 (MQ=255) gcgTTGAGGATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGt > 1:1388338/1‑71 (MQ=255) cgTTGAGGATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTg < 1:1343787/71‑1 (MQ=255) gTTGAGGATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGa > 1:960281/1‑71 (MQ=255) gTTGAGGATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGa < 1:1304082/71‑1 (MQ=255) gTTGAGGATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGa > 1:1586633/1‑71 (MQ=255) aGGATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAg > 1:3182750/1‑71 (MQ=255) aGGATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAg > 1:1664286/1‑71 (MQ=255) gATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCg < 1:3000284/71‑1 (MQ=255) atcatcAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGt < 1:419002/71‑1 (MQ=255) tcatcaGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTgg > 1:2792016/1‑60 (MQ=255) tcatcaGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTc > 1:426058/1‑71 (MQ=255) tcatcaGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTc < 1:1217930/71‑1 (MQ=255) catcaGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTAGTGATAAGCGTCg > 1:678256/1‑71 (MQ=255) atcaGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCgg > 1:2279343/1‑71 (MQ=255) tcaGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGt > 1:1712634/1‑58 (MQ=255) tcaGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCgg > 1:3195469/1‑70 (MQ=255) tcaGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTAGTGATAAGCGTCGGc < 1:2139079/71‑1 (MQ=255) caGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCgg > 1:2689685/1‑69 (MQ=255) aGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCgg > 1:1404582/1‑68 (MQ=255) aGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCgg > 1:799359/1‑68 (MQ=255) aGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCgg > 1:2076051/1‑68 (MQ=255) aGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCgg > 1:302698/1‑68 (MQ=255) cGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGAt < 1:1440817/57‑1 (MQ=255) cGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGc > 1:758924/1‑67 (MQ=255) ggATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCa < 1:3726581/71‑1 (MQ=255) gATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCAt < 1:2864693/71‑1 (MQ=255) aTCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATa < 1:1619492/71‑1 (MQ=255) tCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGt < 1:599340/49‑1 (MQ=255) tCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAg < 1:1491115/71‑1 (MQ=255) cAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTAGTGATAAGCGTCGGCAGACCATAgg > 1:729596/1‑71 (MQ=255) cAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTAGTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAgg > 1:806090/1‑71 (MQ=255) aGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATa < 1:652483/51‑1 (MQ=255) aGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGt < 1:3288763/71‑1 (MQ=255) aGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTAGTGATAAGCGTCGGCAGACCATAGGt < 1:827864/71‑1 (MQ=255) gTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTc < 1:3295637/71‑1 (MQ=255) tttttCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGAt > 1:537250/1‑48 (MQ=255) tttttCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTc > 1:727095/1‑70 (MQ=255) ttttCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCa > 1:1638503/1‑63 (MQ=255) ttttCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTAGTGATAAGCGTCGGCAGACCATAGGTCCg > 1:3945235/1‑71 (MQ=255) tttCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGt > 1:2438254/1‑52 (MQ=255) tttCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCg > 1:3103045/1‑70 (MQ=255) tttCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCg > 1:1005401/1‑70 (MQ=255) tttCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCg > 1:2625676/1‑70 (MQ=255) tttCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGAGTCGGCAGACCATAGGTc > 1:2630319/1‑68 (MQ=255) tCCGGAAATTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCa < 1:3780037/70‑1 (MQ=255) tCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTAGTGATAAGCGTCGGCAGAc > 1:3110723/1‑58 (MQ=255) ccGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGa > 1:160602/1‑42 (MQ=255) ggAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGt < 1:3229562/47‑1 (MQ=255) gAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAg > 1:2285649/1‑59 (MQ=255) aaaGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCg > 1:2064494/1‑47 (MQ=255) aaaGTGGTAAGGGCCGTAGTTATTCGAGCAGTTAGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAgg > 1:828417/1‑71 (MQ=255) aaGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGa > 1:1046866/1‑37 (MQ=255) aaGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGgc < 1:3319536/71‑1 (MQ=255) aaGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCa > 1:252324/1‑42 (MQ=255) aaGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCAc > 1:3310420/1‑70 (MQ=255) aGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCAc > 1:3531733/1‑69 (MQ=255) gggCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAg < 1:2273275/71‑1 (MQ=255) ggCCGTAGTTATTCGAGCAGTTAGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCAc > 1:1763398/1‑67 (MQ=255) gCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAg > 1:1283304/1‑39 (MQ=255) gCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAg > 1:1363343/1‑39 (MQ=255) ccGTAGTTATTCGAGCAGTTAGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATg > 1:3353937/1‑71 (MQ=255) cGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGa > 1:277317/1‑68 (MQ=255) cGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATgg < 1:419273/71‑1 (MQ=255) cGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATgg < 1:348507/71‑1 (MQ=255) cGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATgg < 1:1384066/71‑1 (MQ=255) gTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGAc < 1:3712839/38‑1 (MQ=255) tAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTc < 1:2379781/71‑1 (MQ=255) gTTATTCGAGCAGTTAGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTCg < 1:873011/70‑1 (MQ=255) ttATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTcc < 1:3551273/43‑1 (MQ=255) ttATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAg < 1:102836/51‑1 (MQ=255) ttATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAg < 1:202924/51‑1 (MQ=255) ttATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTCgct > 1:1620384/1‑71 (MQ=255) aTTCGAGCAGTTGGTGATAAGCGTCGGCAGACCAt < 1:3023664/35‑1 (MQ=255) aTTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATa > 1:3141278/1‑36 (MQ=255) aTTCGAGCAGTTAGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTCgctg < 1:3189115/70‑1 (MQ=255) aTTCGACCAGTTGGTGATAAGCGTCGGCAGACCATAg > 1:8208/1‑37 (MQ=255) ttCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTCgctgct > 1:2060761/1‑71 (MQ=255) ttCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTCgctgc < 1:148874/70‑1 (MQ=255) tCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAg > 1:1766989/1‑35 (MQ=255) tCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTc > 1:3751332/1‑38 (MQ=255) tCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAg > 1:1512613/1‑47 (MQ=255) cGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCa > 1:241431/1‑41 (MQ=255) aGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTCGCTGCTGGCt > 1:3255404/1‑71 (MQ=255) gCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTCGCTGCTGGCtt > 1:480241/1‑71 (MQ=255) gCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTCGCTGCTGGCtt > 1:1101003/1‑71 (MQ=255) aGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTCGCTGCTGGCtttt < 1:300643/71‑1 (MQ=255) ttGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGGCGCTGCTGGc < 1:1678709/65‑1 (MQ=255) ggTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTCGCTGCTGGCTTTTGAc < 1:2997325/70‑1 (MQ=255) | GCGCGTTGAGGATCATCAGCGGGATCAGTTTTTCCGGAAAGTGGTAAGGGCCGTAGTTATTCGAGCAGTTGGTGATAAGCGTCGGCAGACCATAGGTCCGCAGCCAGGCGCGCACCAGATGGTCGCTGCTGGCTTTTGAC > minE/2425467‑2425606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |