| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,425,789 | G→A | 6.4% | N101N (AAC→AAT) | rffG ← | dTDP‑glucose 4,6‑dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,425,789 | 0 | G | A | 6.4% | 294.6 / 6.6 | 94 | N101N (AAC→AAT) | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (45/43); new base A (4/2); total (49/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
ATTTTTTATCTTCCGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCAT > minE/2425719‑2425857 | aTTTTTTATCTTCCGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATg > 1:1807915/1‑71 (MQ=255) tttttATCTTCCGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGtt > 1:957597/1‑71 (MQ=255) ttATCTTCCGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGt > 1:349846/1‑71 (MQ=255) ttATCTTCCGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGt > 1:1328281/1‑71 (MQ=255) ccGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTc < 1:1628859/67‑1 (MQ=255) ccGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAAt < 1:1159179/70‑1 (MQ=255) cGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCa > 1:248737/1‑67 (MQ=255) cGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATaa < 1:3219417/71‑1 (MQ=255) cGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATaa < 1:134917/71‑1 (MQ=255) gTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATaaa > 1:357337/1‑71 (MQ=255) tCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATaa < 1:2823633/69‑1 (MQ=255) tCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAAc < 1:104806/71‑1 (MQ=255) tCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAAc < 1:2874746/71‑1 (MQ=255) cAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACg > 1:2291183/1‑71 (MQ=255) aGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGc < 1:1669872/71‑1 (MQ=255) cgcATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTg < 1:1253654/71‑1 (MQ=255) gcATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTgg < 1:1247140/55‑1 (MQ=255) gcATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATaaa > 1:2233087/1‑65 (MQ=255) gcATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTg > 1:3280083/1‑70 (MQ=255) gcATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGc > 1:1393256/1‑71 (MQ=255) gcATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGc > 1:381667/1‑71 (MQ=255) cATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGcc > 1:261162/1‑71 (MQ=255) cATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGcc > 1:350627/1‑71 (MQ=255) aTTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCg > 1:2524158/1‑71 (MQ=255) aTTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCg > 1:2704211/1‑71 (MQ=255) aTTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCg < 1:2777507/71‑1 (MQ=255) ttCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCgg > 1:3226780/1‑71 (MQ=255) tCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGt > 1:2952461/1‑52 (MQ=255) tCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGt > 1:294005/1‑52 (MQ=255) tCCAGTAAGCCCGCGCCGATTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCgg > 1:3884299/1‑70 (MQ=255) ccAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGc > 1:1891702/1‑71 (MQ=255) cAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGc > 1:1035074/1‑65 (MQ=255) cAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGcc < 1:105697/71‑1 (MQ=255) gTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCg < 1:3314546/70‑1 (MQ=255) tAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATaaa < 1:1546688/56‑1 (MQ=255) aaGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTc < 1:1631177/70‑1 (MQ=255) aGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCaa > 1:687279/1‑71 (MQ=255) gCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCaa < 1:2441393/70‑1 (MQ=255) cccGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCaa > 1:529760/1‑69 (MQ=255) ccGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTg > 1:2413329/1‑40 (MQ=255) ccGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAg < 1:1885767/71‑1 (MQ=255) cgcgCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATa > 1:115415/1‑69 (MQ=255) cgcgCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAg > 1:2762674/1‑70 (MQ=255) gcgcCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGaa > 1:264388/1‑71 (MQ=255) gccgcTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACg < 1:699644/71‑1 (MQ=255) gccgcTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACg < 1:1797915/71‑1 (MQ=255) gccgcTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTCCCGGGCCGTCAATAGAACg > 1:1662591/1‑71 (MQ=255) ccgcTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACg < 1:1733003/70‑1 (MQ=255) ctcTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCaa < 1:3052140/39‑1 (MQ=255) gcTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTc < 1:3654842/71‑1 (MQ=255) tCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATaa > 1:2135611/1‑40 (MQ=255) tCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATaa > 1:1793314/1‑40 (MQ=255) tCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAAt < 1:1894911/59‑1 (MQ=255) tCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCaa > 1:834530/1‑70 (MQ=255) cAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTc < 1:1397775/55‑1 (MQ=255) cAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACa > 1:1823733/1‑71 (MQ=255) cAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACa > 1:3807353/1‑71 (MQ=255) cAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACa > 1:1956674/1‑71 (MQ=255) aaGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACa < 1:2355245/70‑1 (MQ=255) aaGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACAt < 1:2614328/71‑1 (MQ=255) aGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACAt < 1:594339/70‑1 (MQ=255) aaTGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACAt < 1:3639418/67‑1 (MQ=255) aaTGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACAt < 1:1425877/67‑1 (MQ=255) aTGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGt < 1:2374994/48‑1 (MQ=255) aTGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCtt > 1:1387052/1‑71 (MQ=255) tGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCttt < 1:2269879/71‑1 (MQ=255) tGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCttt < 1:1103193/71‑1 (MQ=255) gTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTc > 1:2844453/1‑71 (MQ=255) tataAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTc < 1:2995065/70‑1 (MQ=255) cataAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCttt > 1:3209261/2‑69 (MQ=255) cataAGTACCAACAATATTGGTTTCAAT‑AATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTcag < 1:861050/70‑3 (MQ=255) taAGTCCCGACAATGTTGGTTTCAATAAACGCTGcc > 1:1344396/1‑36 (MQ=255) taAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGcctg > 1:1241989/1‑41 (MQ=16) aaGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCgg < 1:762616/69‑1 (MQ=255) aaGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGc > 1:1957527/1‑70 (MQ=255) aGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTg < 1:172685/71‑1 (MQ=255) aGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACg > 1:2668448/1‑51 (MQ=255) gTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTc < 1:3487039/53‑1 (MQ=255) gTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGc > 1:2278821/1‑71 (MQ=255) gTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGc > 1:59207/1‑71 (MQ=255) tCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACAt > 1:3646852/1‑57 (MQ=255) tCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGcc < 1:2126022/71‑1 (MQ=255) ccGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGc > 1:1535743/1‑68 (MQ=255) ccGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCa < 1:851055/70‑1 (MQ=255) ccGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAg < 1:3323253/71‑1 (MQ=255) gACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTc > 1:765827/1‑48 (MQ=255) aCAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTg < 1:764629/71‑1 (MQ=255) cAATGTTGGTTTCAATAAACTCTGCCGGGCCGTCAATAGAACGGTCAAc > 1:3013270/1‑49 (MQ=255) cAATGTTGGTTTCAATAAACGCTGCCTGGCCGTCAATAGAACGGTCAACAt < 1:145190/51‑1 (MQ=255) cAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATg > 1:2659163/1‑70 (MQ=255) cAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATg > 1:1830248/1‑70 (MQ=255) cAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGc < 1:2277127/71‑1 (MQ=255) cAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGc < 1:154459/71‑1 (MQ=255) aaTGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCa < 1:3150288/71‑1 (MQ=255) aTGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCAt > 1:3006506/1‑71 (MQ=255) | ATTTTTTATCTTCCGTCAGCGCATTCCAGTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCAT > minE/2425719‑2425857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |