| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,425,816 | G→T | 6.0% | D92E (GAC→GAA) ‡ | rffG ← | dTDP‑glucose 4,6‑dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,425,816 | 0 | G | T | 6.0% | 329.4 / 4.0 | 99 | D92E (GAC→GAA) ‡ | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (45/48); new base T (3/3); total (48/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAAT > minE/2425747‑2425885 | gTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCg < 1:3314546/70‑1 (MQ=255) aaGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTc < 1:1631177/70‑1 (MQ=255) aGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCaa > 1:687279/1‑71 (MQ=255) gCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCaa < 1:2441393/70‑1 (MQ=255) cccGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCaa > 1:529760/1‑69 (MQ=255) ccGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAg < 1:1885767/71‑1 (MQ=255) cgcgCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATa > 1:115415/1‑69 (MQ=255) cgcgCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAg > 1:2762674/1‑70 (MQ=255) gcgcCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGaa > 1:264388/1‑71 (MQ=255) gccgcTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACg < 1:1797915/71‑1 (MQ=255) gccgcTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACg < 1:699644/71‑1 (MQ=255) gccgcTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTCCCGGGCCGTCAATAGAACg > 1:1662591/1‑71 (MQ=255) ccgcTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACg < 1:1733003/70‑1 (MQ=255) gcTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTc < 1:3654842/71‑1 (MQ=255) tCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAAt < 1:1894911/59‑1 (MQ=255) tCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCaa > 1:834530/1‑70 (MQ=255) cAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTc < 1:1397775/55‑1 (MQ=255) cAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACa > 1:3807353/1‑71 (MQ=255) cAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACa > 1:1956674/1‑71 (MQ=255) cAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACa > 1:1823733/1‑71 (MQ=255) aaGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACa < 1:2355245/70‑1 (MQ=255) aaGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACAt < 1:2614328/71‑1 (MQ=255) aGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACAt < 1:594339/70‑1 (MQ=255) aaTGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACAt < 1:3639418/67‑1 (MQ=255) aaTGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACAt < 1:1425877/67‑1 (MQ=255) aTGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGt < 1:2374994/48‑1 (MQ=255) aTGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCtt > 1:1387052/1‑71 (MQ=255) tGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCttt < 1:1103193/71‑1 (MQ=255) tGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCttt < 1:2269879/71‑1 (MQ=255) gTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTc > 1:2844453/1‑71 (MQ=255) tataAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTc < 1:2995065/70‑1 (MQ=255) cataAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCttt > 1:3209261/2‑69 (MQ=255) cataAGTACCAACAATATTGGTTTCAAT‑AATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTcag < 1:861050/70‑3 (MQ=255) aaGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCgg < 1:762616/69‑1 (MQ=255) aaGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGc > 1:1957527/1‑70 (MQ=255) aGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTg < 1:172685/71‑1 (MQ=255) aGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACg > 1:2668448/1‑51 (MQ=255) gTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTc < 1:3487039/53‑1 (MQ=255) gTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGc > 1:2278821/1‑71 (MQ=255) gTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGc > 1:59207/1‑71 (MQ=255) tCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACAt > 1:3646852/1‑57 (MQ=255) tCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGcc < 1:2126022/71‑1 (MQ=255) ccGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGc > 1:1535743/1‑68 (MQ=255) ccGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCa < 1:851055/70‑1 (MQ=255) ccGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAg < 1:3323253/71‑1 (MQ=255) gACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTc > 1:765827/1‑48 (MQ=255) aCAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTg < 1:764629/71‑1 (MQ=255) cAATGTTGGTTTCAATAAACTCTGCCGGGCCGTCAATAGAACGGTCAAc > 1:3013270/1‑49 (MQ=255) cAATGTTGGTTTCAATAAACGCTGCCTGGCCGTCAATAGAACGGTCAACAt < 1:145190/51‑1 (MQ=255) cAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATg > 1:1830248/1‑70 (MQ=255) cAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATg > 1:2659163/1‑70 (MQ=255) cAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGc < 1:154459/71‑1 (MQ=255) cAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGc < 1:2277127/71‑1 (MQ=255) aaTGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCa < 1:3150288/71‑1 (MQ=255) aTGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCAt > 1:3006506/1‑71 (MQ=255) ttGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTcag < 1:2282845/55‑3 (MQ=255) ttGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCttt < 1:1346486/52‑1 (MQ=255) ttGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAg < 1:1890879/62‑1 (MQ=255) ggTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGAt < 1:2775507/62‑1 (MQ=255) gTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGacac < 1:1047876/70‑1 (MQ=255) tttCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGcc < 1:3461569/56‑1 (MQ=255) ttCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGc < 1:3480487/61‑1 (MQ=255) tCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTc > 1:2886854/1‑71 (MQ=255) tCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTc > 1:3920947/1‑71 (MQ=255) tCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTc > 1:3741151/1‑71 (MQ=255) tCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTc > 1:1634468/1‑71 (MQ=255) tCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTc > 1:359633/1‑71 (MQ=255) cAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCgg > 1:734671/1‑48 (MQ=255) cAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATTACACAGTCt > 1:531413/1‑71 (MQ=255) aaTAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCgg < 1:405941/47‑1 (MQ=255) tAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCg < 1:1968123/44‑1 (MQ=255) tAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGt > 1:2637057/1‑66 (MQ=255) tAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGc > 1:2040312/1‑71 (MQ=255) aaaCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTgg > 1:2017319/1‑69 (MQ=255) aaaCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTgg > 1:3948973/1‑69 (MQ=255) aaaCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTg > 1:2850337/1‑68 (MQ=255) aaaCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCt > 1:3240864/1‑71 (MQ=255) aaaCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCt > 1:3074137/1‑71 (MQ=255) aaaCGCTGCCGGGCCGTCAATAGAACGGTCAAAATGGCTTTCGGCTGCCAGATGCATGACACAGTCTgg > 1:982845/1‑69 (MQ=255) aaCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCtt > 1:731789/1‑39 (MQ=255) aCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGAt > 1:882756/1‑51 (MQ=255) aCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGAt > 1:1190247/1‑51 (MQ=255) gCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCt < 1:1771928/63‑1 (MQ=255) ccGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGAt > 1:3786143/1‑66 (MQ=255) ccGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCt < 1:1513662/69‑1 (MQ=255) ccGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCt > 1:2919334/1‑69 (MQ=255) ccGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCt > 1:2370404/1‑69 (MQ=255) ccGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCt < 1:1420360/69‑1 (MQ=255) ccGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTc < 1:3039585/70‑1 (MQ=255) ccGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTc < 1:1531255/70‑1 (MQ=255) ccGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTc < 1:290285/70‑1 (MQ=255) gggCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTc > 1:172826/1‑68 (MQ=255) ggCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTg < 1:3205400/71‑1 (MQ=255) gCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGa < 1:2918446/71‑1 (MQ=255) ccGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCa > 1:2026529/1‑66 (MQ=255) cGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGaa > 1:1681617/1‑70 (MQ=255) cGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGa < 1:967302/69‑1 (MQ=255) cGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAAt < 1:1474585/71‑1 (MQ=255) cGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAAt < 1:1227328/71‑1 (MQ=255) cGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAAt < 1:1491050/71‑1 (MQ=255) | GTAAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAAT > minE/2425747‑2425885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |