| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,429,480 | C→T | 16.0% | G35S (GGC→AGC) | wzzE ← | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,429,480 | 0 | C | T | 16.0% | 211.0 / 28.8 | 88 | G35S (GGC→AGC) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base C (41/32); new base T (10/4); total (52/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TCGAGCTCCACTCCTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCATTTTCAGCGT > minE/2429411‑2429548 | tCGAGCTCCACTCCTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGcc > 1:2020785/1‑70 (MQ=255)tCGAGCTCCACTCCTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCa < 1:1673006/71‑1 (MQ=255)tCGAGCTCCACTCCTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCa < 1:2209017/71‑1 (MQ=255) aGCTCCACTCCTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCaat > 1:65153/1‑70 (MQ=255) cTCCACTCCTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCaataat > 1:1136961/1‑71 (MQ=255) cTCCACTCCTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCaataat > 1:479359/1‑71 (MQ=255) ccACTCCTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATc > 1:3834058/1‑70 (MQ=255) cACTCCTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATcc < 1:197803/70‑1 (MQ=255) cTCCTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCTAATAATCCAt > 1:1855627/1‑70 (MQ=255) cTCCTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCTAATAATCCAt > 1:2118356/1‑70 (MQ=255) tCCTGACGAGCAAAAAACGTATACGCCAGAGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAg > 1:1290007/1‑71 (MQ=255) cTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCTAATAATCCATAGCt > 1:2803543/1‑71 (MQ=255) cTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCa < 1:3475067/58‑1 (MQ=255) cTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCt > 1:395363/1‑71 (MQ=255) cTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCt > 1:2830878/1‑71 (MQ=255) cTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCt > 1:1829799/1‑71 (MQ=255) gACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCaat > 1:2653848/1‑58 (MQ=255) aCGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCaat < 1:1073602/57‑1 (MQ=255) aCGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTcc > 1:2819177/1‑71 (MQ=255) aCGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTcc > 1:2861312/1‑71 (MQ=255) aCGAGAAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCtt < 1:1517323/69‑1 (MQ=255) cAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGcc > 1:2033191/1‑71 (MQ=255) aaaaaCGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCaa > 1:3527424/1‑49 (MQ=255) aaaaaCGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCca > 1:65479/1‑71 (MQ=255) aaaaCGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCa > 1:383896/1‑55 (MQ=255) aaaaCGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCca > 1:3219029/1‑70 (MQ=255) aaaaCGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGACAATAATCCATAGCTTCCCAGCCcac < 1:1823927/71‑1 (MQ=255) aaaCGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCTaa > 1:2633466/1‑47 (MQ=255) aaCGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCcac > 1:955246/1‑69 (MQ=255) aCGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAg < 1:2585339/71‑1 (MQ=255) aCGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAg < 1:363480/71‑1 (MQ=255) gTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCa < 1:3108514/50‑1 (MQ=255) gTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAgg < 1:1137368/70‑1 (MQ=255) tataCGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCcaca < 1:2722100/66‑1 (MQ=255) ataCGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCTAATAATCCATAGCTTCCCAGCCCACAAGGTAc > 1:182714/1‑71 (MQ=255) taCGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACg < 1:981299/71‑1 (MQ=255) taCGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCAAGCCCACAAGGTAc > 1:3341055/1‑70 (MQ=255) taCGCCAGCGCGATTAACGCAAACGCAAGCCCCATGCCAATAATCCATAGCTTCCCa > 1:2925559/1‑57 (MQ=255) cGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCTAATAATCCATAGCTTCCCAGCCCACAAGGTACGa > 1:294967/1‑70 (MQ=255) cGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGa < 1:1348779/70‑1 (MQ=255) cGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGa < 1:16093/70‑1 (MQ=255) cGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGa > 1:1687953/1‑70 (MQ=255) gCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATa < 1:2485293/46‑1 (MQ=255) ccAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGaaa > 1:1295693/1‑70 (MQ=255) aGCGCGATTAACGCAAACGCCAGCCCCATGCCaat < 1:3916643/35‑1 (MQ=255) gcgcGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAaacaac > 1:2830535/1‑71 (MQ=255) gcgcGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAaacaac < 1:107407/71‑1 (MQ=255) cgATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGaa > 1:1207598/1‑63 (MQ=255) cgATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCa < 1:1289746/71‑1 (MQ=255) gATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCAc > 1:1693980/1‑71 (MQ=255) aTTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACg < 1:1605714/71‑1 (MQ=255) aTTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACg < 1:948920/71‑1 (MQ=255) aTTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACg < 1:1870037/71‑1 (MQ=255) ttAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACg > 1:765288/1‑70 (MQ=255) tAACGCAAACGCCAGCCCCATGCTAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGaa > 1:3576378/1‑71 (MQ=255) tAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCa > 1:2341114/1‑43 (MQ=255) tAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGt > 1:2583744/1‑54 (MQ=255) tAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGaaa > 1:1502394/1‑60 (MQ=255) aCGCAAACGCCAGCCCCATGCTAATAATCCATAGCTTCCCAGCCCACAAg < 1:1132734/50‑1 (MQ=255) aCGCAAACGCCAGCCCCATGCTAATAATCCATAGCTTCCCAGCCCACAAg < 1:339835/50‑1 (MQ=255) aCGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCa > 1:2760659/1‑41 (MQ=255) gCAAACGCCAGCCCCATGCCAATAATCCATAGCTTccc > 1:3186164/1‑38 (MQ=255) gCAAACGCCAGCCCCATGCCAATAATCCATACCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATc < 1:414261/71‑1 (MQ=255) cAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTAc > 1:1432917/1‑51 (MQ=255) cAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATc > 1:2413410/1‑70 (MQ=255) aaCGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAg > 1:2345720/1‑71 (MQ=255) aCGCCAGCCCCATGCTAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGt > 1:3865639/1‑71 (MQ=255) aCGCAAGCCCCATGCAAATAATCCATAGCTTCCCAGcca > 1:2366814/1‑38 (MQ=38) cGCCAGCCCCATGCTAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGtt > 1:2304665/1‑71 (MQ=255) gCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTc < 1:1823058/71‑1 (MQ=255) ccAGCCCCATGCTAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGt > 1:3057185/1‑68 (MQ=255) cAGCCCCATGCTAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCa < 1:3583621/70‑1 (MQ=255) cAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAgg < 1:2306898/41‑1 (MQ=255) aGCCCCATGCCAATAATCCATAGCTTCCCAGCCcaca < 1:2076191/37‑1 (MQ=255) aGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCAtt < 1:1086085/71‑1 (MQ=255) gCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTAc > 1:335699/1‑42 (MQ=255) gCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCAttt < 1:3500308/71‑1 (MQ=255) gCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCAttt > 1:1347947/1‑71 (MQ=255) gCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCAttt > 1:1455557/1‑71 (MQ=255) gCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCAttt > 1:2669261/1‑71 (MQ=255) ccccATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCAtttt < 1:1568313/71‑1 (MQ=255) cccATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCAtttt < 1:3177075/70‑1 (MQ=255) cccATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCATTTTc < 1:1312170/71‑1 (MQ=255) cccATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCATTTTc < 1:1980441/71‑1 (MQ=255) ccATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAatat > 1:567615/1‑57 (MQ=255) cATGCCAATAATCCATAGCTTCCCAGCCCACAAgg > 1:2669534/1‑35 (MQ=255) cATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCATTTTCAg > 1:1242549/1‑71 (MQ=255) cccccAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCATTTTCa > 1:756140/4‑69 (MQ=255) aTGCCAATAATCCATAGCTTCCCAGCCCACAAGGTa > 1:833488/1‑36 (MQ=255) tGCTAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCAtt < 1:744157/64‑1 (MQ=255) ccAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCATTTTCAGCGt < 1:1975328/70‑1 (MQ=255) cAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAg < 1:252561/55‑1 (MQ=255) | TCGAGCTCCACTCCTGACGAGCAAAAAACGTATACGCCAGCGCGATTAACGCAAACGCCAGCCCCATGCCAATAATCCATAGCTTCCCAGCCCACAAGGTACGAAACAACCCACGAATATCCAGTTCATTTTCAGCGT > minE/2429411‑2429548 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |