| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,430,365 | T→C | 45.2% | I111M (ATA→ATG) | rfe ← | UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,430,365 | 0 | T | C | 45.2% | 11.9 / 79.3 | 62 | I111M (ATA→ATG) | rfe | UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase |
| Reads supporting (aligned to +/- strand): ref base T (20/14); new base C (13/15); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01 | |||||||||||
CCGAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCAGC > minE/2430297‑2430434 | ccGAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATta > 1:530950/1‑70 (MQ=255) cGAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATtat > 1:3246988/1‑70 (MQ=255) cGAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATtat < 1:2649503/70‑1 (MQ=255) cGAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATtat < 1:39854/70‑1 (MQ=255) cGAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATtat < 1:449073/70‑1 (MQ=255) gaacACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATtat < 1:2056754/66‑1 (MQ=255) gCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACa < 1:2931554/71‑1 (MQ=255) aCCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAAt < 1:616394/71‑1 (MQ=255) cATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATtat < 1:2497100/63‑1 (MQ=255) tctcCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGcc > 1:411343/1‑70 (MQ=255) ccAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATtata < 1:2997940/58‑1 (MQ=255) ccAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCaa < 1:2055069/68‑1 (MQ=255) aGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCg > 1:3022482/1‑71 (MQ=255) aGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCg > 1:3419524/1‑71 (MQ=255) aGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCg < 1:3702487/71‑1 (MQ=255) aGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCg < 1:2772344/71‑1 (MQ=255) ggAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCg < 1:1805364/70‑1 (MQ=255) ccAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGcc > 1:3229912/1‑59 (MQ=255) aaaGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTa < 1:1004658/71‑1 (MQ=255) aaaGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTa < 1:1051562/71‑1 (MQ=255) aaaGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTa > 1:3702551/1‑71 (MQ=255) aaGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATg < 1:1520545/54‑1 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTAtg > 1:1628340/1‑71 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTAtg > 1:1111434/1‑71 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATg < 1:1076174/53‑1 (MQ=255) gATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACa < 1:1159066/49‑1 (MQ=255) gATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGcc > 1:2222422/1‑54 (MQ=255) gATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTAtgg > 1:328943/1‑71 (MQ=255) ataACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTa < 1:1177442/65‑1 (MQ=255) taACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTAtggtgg < 1:2065153/70‑1 (MQ=255) cccAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACg > 1:740747/1‑71 (MQ=255) ccAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACa < 1:2460143/50‑1 (MQ=255) ccAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACa < 1:441269/50‑1 (MQ=255) ggCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtt > 1:3351822/1‑71 (MQ=255) gCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAttt < 1:666280/71‑1 (MQ=255) gCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTAtggt < 1:2526485/60‑1 (MQ=255) cTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTa > 1:2263930/1‑55 (MQ=255) cTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtttt > 1:3887394/1‑71 (MQ=255) tACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGc > 1:847405/1‑46 (MQ=255) tACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtttt > 1:3194303/1‑70 (MQ=255) tACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtttt > 1:2301753/1‑70 (MQ=255) tACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtttt > 1:1456874/1‑70 (MQ=255) cTGAGATAAAGCTTGCCGAACACCATCATAACAATGcc < 1:698754/38‑1 (MQ=255) tGAGATAAAGCTTGCCGAACACCATCATAACAATGcc < 1:247316/37‑1 (MQ=255) gagaTAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGt > 1:1031309/1‑49 (MQ=255) gagaTAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGt > 1:3202306/1‑49 (MQ=255) gagaTAAAGCTTGCCGAACACCATCATAACAATGCCAACAg > 1:1998880/1‑41 (MQ=255) agaTAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGTATTTTTACGCt < 1:3865893/71‑1 (MQ=255) gaTAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCg < 1:2891928/41‑1 (MQ=255) gaTAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCg < 1:2075274/41‑1 (MQ=255) aaaGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGata > 1:809726/1‑71 (MQ=255) aaaGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTAtggtgg > 1:877963/1‑51 (MQ=255) aaaGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTAtgg > 1:2581099/1‑48 (MQ=255) aaaGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtt < 1:1721548/59‑1 (MQ=255) aGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGatat > 1:3182296/1‑70 (MQ=255) ttGCCGAACACCATTATAACAATGCCAACAGCGGCCt > 1:2094376/1‑37 (MQ=255) ttGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCaa < 1:872748/70‑1 (MQ=255) gCCGAACACCATTATAACAATGCCAACAGCGGCCTGTAtggtg > 1:1782349/1‑43 (MQ=255) ccGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACg < 1:2469688/71‑1 (MQ=255) cGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGata > 1:3401858/1‑62 (MQ=255) aaCACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAttt > 1:3351420/1‑49 (MQ=255) aaCACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAttt > 1:2387357/1‑49 (MQ=255) acacCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCa < 1:202076/71‑1 (MQ=255) acacCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCa < 1:1690225/71‑1 (MQ=255) acacCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCa < 1:700376/71‑1 (MQ=255) cacCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCAt > 1:2826386/1‑71 (MQ=255) cATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAt > 1:1298250/1‑42 (MQ=255) cATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCa < 1:1036553/71‑1 (MQ=255) aTTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTc > 1:3839558/1‑65 (MQ=255) aTTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTc > 1:981462/1‑65 (MQ=255) ttATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCAgc > 1:608175/1‑71 (MQ=255) ttATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCAgc > 1:559657/1‑71 (MQ=255) ttATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCAgc > 1:975040/1‑71 (MQ=255) tataACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTc > 1:665075/1‑63 (MQ=255) | CCGAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCAGC > minE/2430297‑2430434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |