Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,477,458 T→C 21.3% V168V (GTT→GTC pstA → phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,477,4580TC21.3% 118.1 / 28.3 61V168V (GTT→GTCpstAphosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (19/29);  new base C (7/6);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 5.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCGCAGATGGAGCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTATGCGCTG  >  minE/2477388‑2477524
                                                                      |                                                                  
tggCGCAGATGGAGCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGtt                                                                    <  1:732492/71‑1 (MQ=255)
 ggCGCAGATGGAGCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGtt                                                                    >  1:2546988/1‑70 (MQ=255)
  gcgcAGATGGAGCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTAt                                                                  <  1:3684212/71‑1 (MQ=255)
  gcgcAGATGGAGCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCAt                                                                  >  1:3141507/1‑71 (MQ=255)
   cgcAGATGGAGCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTAt                                                                  <  1:1015911/70‑1 (MQ=255)
   cgcAGATGGAGCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATc                                                                 >  1:632880/1‑71 (MQ=255)
    gcAGATGGAGCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATc                                                                 >  1:652125/1‑70 (MQ=255)
      aGATGGAGCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCg                                                               >  1:1500903/1‑70 (MQ=255)
             gCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCg                                                       <  1:3505377/71‑1 (MQ=255)
                      cGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATg                                               <  1:317038/70‑1 (MQ=255)
                      cGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGc                                              <  1:2868292/71‑1 (MQ=255)
                        gCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCt                                             >  1:2605501/1‑70 (MQ=255)
                        gCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTg                                            <  1:1163959/71‑1 (MQ=255)
                          tGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGa                                           >  1:753940/1‑70 (MQ=255)
                            ggcgggcgTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAAc                                        <  1:2297666/71‑1 (MQ=255)
                              cgggcgTGATTGCCCTGGCGTTGTTGAAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTg                                      >  1:875377/1‑71 (MQ=255)
                                 gcgTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTg                                   >  1:1382246/1‑71 (MQ=255)
                                   gTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGcc                                 >  1:3255322/1‑71 (MQ=255)
                                    tGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGtt                                                                    >  1:247428/1‑35 (MQ=255)
                                    tGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGcc                                 >  1:260889/1‑70 (MQ=255)
                                      aTTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGt                               <  1:1678593/70‑1 (MQ=255)
                                      aTTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTa                              >  1:687274/1‑71 (MQ=255)
                                      aTTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGt                               <  1:922357/70‑1 (MQ=255)
                                       ttGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTAc                             <  1:2862616/71‑1 (MQ=255)
                                       ttGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTa                              >  1:3684545/1‑70 (MQ=255)
                                           ccTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGcc                         <  1:154843/71‑1 (MQ=255)
                                           ccTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGcc                         <  1:816007/71‑1 (MQ=255)
                                           ccTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGcc                         <  1:1374763/71‑1 (MQ=255)
                                           ccTGGCGTTGTTGCAGGTGACGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGcc                         <  1:1360320/71‑1 (MQ=255)
                                            cTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCt                        >  1:309857/1‑71 (MQ=255)
                                              ggCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTAc                             <  1:1511447/64‑1 (MQ=255)
                                               gCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCg                     >  1:1448594/1‑71 (MQ=255)
                                                cGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTgg                                     >  1:1075149/1‑54 (MQ=255)
                                                 gttgttGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCg                                <  1:285763/58‑1 (MQ=255)
                                                  ttgttgCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa                  <  1:2312677/71‑1 (MQ=255)
                                                   tgttgCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAAc                                        >  1:2950975/1‑48 (MQ=255)
                                                     ttgCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGc               <  1:798604/71‑1 (MQ=255)
                                                       gCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCgg             >  1:3534877/1‑71 (MQ=255)
                                                         aGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGcc                                 <  1:2923081/49‑1 (MQ=255)
                                                         aGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGcc                                 <  1:1835729/49‑1 (MQ=255)
                                                           gTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCg                     <  1:2132570/59‑1 (MQ=255)
                                                           gTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCg                     <  1:3376881/59‑1 (MQ=255)
                                                           gTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTa         <  1:960279/71‑1 (MQ=255)
                                                           gTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTa         <  1:193074/71‑1 (MQ=255)
                                                            tGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCt                        <  1:1540317/55‑1 (MQ=255)
                                                             gCCGATTGTTATCCGCACCACCTAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGTCTTa         <  1:3183667/69‑1 (MQ=255)
                                                             gCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa                  >  1:3639433/1‑60 (MQ=255)
                                                             gCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa                  >  1:3080509/1‑60 (MQ=255)
                                                             gCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTATg       >  1:1774199/1‑71 (MQ=255)
                                                              ccGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTATgc      <  1:3312568/71‑1 (MQ=255)
                                                              ccGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTATg       <  1:2222710/70‑1 (MQ=255)
                                                               cGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACa                            >  1:2208532/1‑48 (MQ=255)
                                                               cGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCg                     <  1:2887635/55‑1 (MQ=255)
                                                               cGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCg                     <  1:923391/55‑1 (MQ=255)
                                                               cGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCg                     <  1:1740054/55‑1 (MQ=255)
                                                               cGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTATgcg     <  1:2948601/71‑1 (MQ=255)
                                                                gATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGaa                 >  1:3075751/1‑58 (MQ=255)
                                                                gATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGaa                 >  1:474693/1‑58 (MQ=255)
                                                                gATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa                  <  1:1300773/57‑1 (MQ=255)
                                                                 aTTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGaa                 <  1:288020/57‑1 (MQ=255)
                                                                  ttGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGcc                         <  1:1100086/48‑1 (MQ=255)
                                                                  ttGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTATGCGCTg  >  1:36352/1‑71 (MQ=255)
                                                                  ttGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTATGCGCTg  >  1:3042489/1‑71 (MQ=255)
                                                                  ttGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTATgc      >  1:3515704/1‑67 (MQ=255)
                                                                     ttATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTa         <  1:3608623/61‑1 (MQ=255)
                                                                      tATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa                  <  1:2743133/51‑1 (MQ=255)
                                                                      |                                                                  
TGGCGCAGATGGAGCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTATGCGCTG  >  minE/2477388‑2477524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: