| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,477,887 | A→G | 21.4% | intergenic (+42/‑141) | pstA → / → pstB | phosphate transporter subunit/phosphate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,477,887 | 0 | A | G | 21.4% | 51.4 / 9.0 | 28 | intergenic (+42/‑141) | pstA/pstB | phosphate transporter subunit/phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (14/8); new base G (3/3); total (17/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTTGCGAAGAATAAACACGGTTGATATTGCTGACACGGTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACGGTTAACACTTTGCCGGATGCGGC > minE/2477820‑2477955 | agcgtcgcatctggcataaacaaagcgcactttgctggtctgTTCCCCTCACCCTAACCATCTCCCCggag < 1:2856639‑M2/29‑1 (MQ=255) tGCGAAGAATAAACACGGTTGATATTGCTGACACGGTTTTCCCCTCACCCTAACCCTCTCCCCAGAggg > 1:2999350/1‑69 (MQ=255) cGAAGAATAAACACGGTTGATATTGCTGACACGGTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCg < 1:200668/70‑1 (MQ=255) gaagaaTAAACACGGTTGATATTGCTGACACGGTTTTCCCCTCACCCTAACCATCTCCCCAGAgg < 1:2244658/65‑1 (MQ=255) agaaTAAACACGGTTGATATTGCTGACACGGTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAggg < 1:1912480/71‑1 (MQ=255) gcataaacaaagcgcactttgctggtctgTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCg < 1:809041‑M2/34‑1 (MQ=255) gcataaacaaagcgcactttgctggtctgTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGga < 1:3771594‑M2/40‑1 (MQ=255) taaacaaagcgcactttgctggtctgTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGa > 1:3350532‑M2/27‑61 (MQ=255) aaacaaagcgcactttgctggtctgTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGCggg > 1:1333525‑M2/26‑63 (MQ=255) aaacaaagcgcactttgctggtctgTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAggggactgt > 1:3146473‑M2/26‑66 (MQ=255) caaagcgcactttgctggtctgTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGggactgtccggg < 1:3898565‑M2/49‑3 (MQ=255) gTTGATATTGCTGACACGGTTTTCCCCTCACCCTAACCCTCTCCCCagag > 1:1083685/1‑50 (MQ=255) tGATATTGCTGACACGGTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa > 1:3862328/1‑54 (MQ=255) tttCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGCCCGAGCGCCGTTTTGACTCTGTaca > 1:1447284/1‑70 (MQ=255) ccccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACgg < 1:3950522/70‑1 (MQ=255) aCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACGGTTAacac > 1:628040/1‑71 (MQ=255) cccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGACCGAGCGCCttttt < 1:3423192/49‑1 (MQ=255) ccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGActc < 1:1735534/53‑1 (MQ=255) ccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACGGTTAACACtt > 1:1166628/1‑71 (MQ=255) tAACCCTCTCCCCAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACGGTTAACACTTTg > 1:34755/1‑71 (MQ=255) tAAACCTCTCCCCAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACGGTTAACACTTTg > 1:1577865/1‑71 (MQ=255) aaCCCTCTCCCCAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTAAACGGTTAACACTTTGc > 1:3945448/1‑71 (MQ=255) aCCCTCTCCCCAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACGGTTAac > 1:1304699/1‑63 (MQ=255) ccTCTCCCCAGAGGGGCGAGGGGCCCGACCGAGCGCCTTTTTGACTCTGTACACGGTTAACACTTTg > 1:2318072/1‑67 (MQ=255) tctcCCCAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACgg > 1:1823239/1‑54 (MQ=255) tcCCCAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACGGTTAACACTTGGCCGGAt > 1:3777795/1‑69 (MQ=255) cccAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACGGTTAACACTTTGCCGGATg < 1:1147372/68‑1 (MQ=255) ccAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACGGTTAACACTTTGCCGGATgcggc > 1:3003638/1‑71 (MQ=255) ccAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACGGTTAACACTTTGCCGGATgcggc < 1:2706250/71‑1 (MQ=255) cAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACGGTTaa > 1:488727/1‑52 (MQ=255) | TTTTTGCGAAGAATAAACACGGTTGATATTGCTGACACGGTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGACCGAGCGCCTTTTTGACTCTGTACACGGTTAACACTTTGCCGGATGCGGC > minE/2477820‑2477955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |