| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,550 | A→T | 10.5% | A441A (GCT→GCA) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,550 | 0 | A | T | 10.5% | 194.4 / 12.9 | 75 | A441A (GCT→GCA) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (37/30); new base T (4/4); total (41/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
CACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACA > minE/311480‑311619 | cACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCaga < 1:136443/71‑1 (MQ=255) aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCagag < 1:2957357/71‑1 (MQ=255) aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCagag < 1:3841463/71‑1 (MQ=255) cAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCaga > 1:2827285/1‑68 (MQ=255) cAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAACATCGCGATACCGACCAGAgc > 1:3517937/1‑70 (MQ=255) aGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTgcg > 1:3705393/1‑70 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:556739/1‑71 (MQ=255) gTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGcc > 1:2353725/1‑71 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:3370656/1‑70 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:3637324/1‑70 (MQ=255) aaCCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGa < 1:3401176/71‑1 (MQ=255) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGaa > 1:2304363/1‑71 (MQ=255) cGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAAtt < 1:3165198/71‑2 (MQ=255) gCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAAttt > 1:3818268/1‑69 (MQ=255) gCCAAAGAAGGCCATCGGTACTAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:3610260/71‑1 (MQ=255) gCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:2487857/71‑1 (MQ=255) cgaagaatGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGc > 1:2403519/3‑71 (MQ=255) cAAAGAAGGCCATCGGTACGAATACCGCCGACACTACCATCGCGATACCGACCAGAGCGCCCTGa > 1:2256322/1‑65 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcc > 1:403912/1‑71 (MQ=255) aagaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGccc > 1:1450726/1‑71 (MQ=255) agaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTg > 1:2517569/1‑61 (MQ=255) aagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCAt > 1:3391678/1‑71 (MQ=255) ggCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGaa > 1:2542684/1‑59 (MQ=255) ggCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCg < 1:2045518/71‑1 (MQ=255) ccATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGat > 1:976105/1‑70 (MQ=255) cATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:1269278/55‑1 (MQ=255) tCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:1606618/57‑1 (MQ=255) tCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTa > 1:361136/1‑71 (MQ=255) cGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc > 1:905829/1‑71 (MQ=255) cGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc > 1:901156/1‑71 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc < 1:2000163/70‑1 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc < 1:2640374/70‑1 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg > 1:2299603/1‑71 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg > 1:3086170/1‑71 (MQ=255) tACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACggg < 1:1733761/71‑1 (MQ=255) cGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGa > 1:709963/1‑61 (MQ=255) cGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACggg > 1:3645596/1‑69 (MQ=255) cGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGt < 1:611625/70‑1 (MQ=255) gAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGaa < 1:1139968/47‑1 (MQ=255) tACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGctt < 1:1490520/71‑1 (MQ=255) aCCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGAttttt < 1:3933907/61‑3 (MQ=255) ccgccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttct < 1:3703952/71‑1 (MQ=255) ccgccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttc < 1:1845213/70‑1 (MQ=255) ccgACAGTACGATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACtt < 1:2372600/55‑1 (MQ=255) ccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:293599/42‑1 (MQ=255) ccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt > 1:3026723/1‑71 (MQ=255) ccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt > 1:3561011/1‑71 (MQ=255) ccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt > 1:117307/1‑71 (MQ=255) cgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACtt < 1:1856760/54‑1 (MQ=255) cgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTg < 1:1364338/71‑1 (MQ=255) gACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACgg > 1:209257/1‑57 (MQ=255) gACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTgg < 1:161871/71‑1 (MQ=255) gTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcccc > 1:307287/1‑41 (MQ=255) gTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGa < 1:350186/46‑1 (MQ=255) gTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGa > 1:1192713/1‑46 (MQ=255) tACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTggcggc > 1:2325397/1‑70 (MQ=255) tACCATCGCGATACCGACAAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCa < 1:2533555/71‑1 (MQ=255) caCCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCg < 1:3603010/43‑1 (MQ=19) ccATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTggcg > 1:411462/1‑66 (MQ=255) aTCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAAcc < 1:1496248/71‑1 (MQ=255) aTCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAAcc > 1:3429363/1‑71 (MQ=255) tCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt < 1:1957728/59‑1 (MQ=255) tCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACct > 1:962547/1‑71 (MQ=255) ctcgATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACTGGt < 1:3882380/46‑1 (MQ=25) gcgATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACctt < 1:2469835/70‑1 (MQ=255) gATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTggc < 1:2242494/58‑1 (MQ=255) aTACCGACCAGAGCGCCCTGAATCTGCCCCATCGACtt < 1:3570617/38‑1 (MQ=255) aTACCGACCAGAGCGCCCTGAATCTGCCCCATCGACtt < 1:2647006/38‑1 (MQ=255) aTACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCttcttc < 1:3357675/71‑1 (MQ=255) tACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAAc > 1:2301690/1‑63 (MQ=255) tACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACcttc < 1:881262/67‑1 (MQ=255) aCCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCttt > 1:395997/1‑51 (MQ=255) cGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGcc < 1:457362/71‑1 (MQ=255) gACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc > 1:3423337/1‑35 (MQ=255) gACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGctt > 1:1352780/1‑44 (MQ=255) aCCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCa > 1:1969391/1‑70 (MQ=255) ccAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCAt > 1:62895/1‑70 (MQ=255) cAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATaa > 1:3859148/1‑71 (MQ=255) cAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATa > 1:3353268/1‑70 (MQ=255) agCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGcc < 1:3562030/64‑1 (MQ=255) agCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAaca < 1:2813696/70‑1 (MQ=255) | CACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACA > minE/311480‑311619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |