| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,493,652 | A→C | 13.5% | intergenic (+34/‑341) | gyrB → / → ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,493,652 | 0 | A | C | 13.5% | 116.9 / 15.3 | 52 | intergenic (+34/‑341) | gyrB/ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Reads supporting (aligned to +/- strand): ref base A (19/26); new base C (3/4); total (22/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
AGAGAACGCCCTGAAAGCGGCGAATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGT > minE/2493582‑2493721 | agagAACGCCCTGAAAGCGGCGAATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTa > 1:960644/1‑71 (MQ=255) aCGCCCTGAAAGCGGCGAATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCtt < 1:3768355/71‑1 (MQ=255) aCGCCCTGAAAGCGGCGAATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCtt < 1:3451787/71‑1 (MQ=255) aCGCCCTGAAAGCGGCGAATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCtt < 1:1938221/71‑1 (MQ=255) cGCCCTGAAAGCGGCGAATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTa < 1:3753495/71‑1 (MQ=255) gCCCTGAAAGCGGCGAATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTAt < 1:1334290/71‑1 (MQ=255) cccTGAAAGCGGCGAATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCtt > 1:188820/1‑68 (MQ=255) gcggcgAATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGcc > 1:1506991/1‑68 (MQ=255) ggcgAATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTa < 1:3703127/59‑1 (MQ=255) taaaTATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATc > 1:3011991/3‑59 (MQ=255) taaaTATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCAGATGCGCTACGCTTATc > 1:2572793/3‑59 (MQ=255) aaTATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGaaaa > 1:372067/1‑70 (MQ=255) atatCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCa > 1:1409141/1‑57 (MQ=255) atatCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAg < 1:150729/58‑1 (MQ=255) tatCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCt > 1:2587836/1‑71 (MQ=255) tatCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCt < 1:3694531/71‑1 (MQ=255) tCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCtt < 1:3356194/70‑1 (MQ=255) gATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCa < 1:3012516/71‑1 (MQ=255) tttAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATc < 1:1464544/62‑1 (MQ=255) tttAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAAt < 1:2921712/69‑1 (MQ=255) tAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTAt > 1:416423/1‑44 (MQ=255) tAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTAt > 1:2190821/1‑44 (MQ=255) tAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTa < 1:359626/70‑1 (MQ=255) gcTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGc < 1:3775165/57‑1 (MQ=255) gcTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAAttt < 1:2201371/71‑1 (MQ=255) gCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg > 1:177543/1‑69 (MQ=255) gCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTa < 1:928018/71‑1 (MQ=255) aatGCCTGATGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAg > 1:2701367/3‑71 (MQ=14) atGCCTGATGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAgg < 1:1678206/70‑1 (MQ=14) tGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa > 1:2386982/1‑52 (MQ=255) tGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa > 1:6608/1‑52 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCtt > 1:2278556/1‑38 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCg > 1:741336/1‑71 (MQ=255) cTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAAtttt < 1:1089963/53‑1 (MQ=255) cTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCg > 1:229203/1‑70 (MQ=255) gATGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGa > 1:277434/1‑70 (MQ=14) aTGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGAt < 1:1366568/70‑1 (MQ=14) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt < 1:1127597/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt > 1:1751399/1‑70 (MQ=255) tGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAGTGTATTGAATTTGCACGATTTTGTAGGCCGGAt > 1:3531496/1‑69 (MQ=14) tGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt > 1:3830590/1‑69 (MQ=255) cgcTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAg < 1:1473834/70‑1 (MQ=14) cgcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAAttt < 1:3251488/46‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAAttt < 1:318566/46‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAgg < 1:1981069/71‑1 (MQ=255) gcTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAAtttg < 1:2458354/46‑2 (MQ=14) gcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAtttt < 1:2128235/55‑1 (MQ=255) gcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg > 1:2761066/1‑56 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAt < 1:2565870/51‑1 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAt < 1:1559875/51‑1 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAt < 1:1709508/51‑1 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCg < 1:3240778/62‑1 (MQ=255) tACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGt > 1:1674431/1‑71 (MQ=255) | AGAGAACGCCCTGAAAGCGGCGAATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGT > minE/2493582‑2493721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |