| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,493,676 | T→C | 24.6% | intergenic (+58/‑317) | gyrB → / → ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,493,676 | 0 | T | C | 24.6% | 117.6 / 32.5 | 57 | intergenic (+58/‑317) | gyrB/ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Reads supporting (aligned to +/- strand): ref base T (16/27); new base C (6/8); total (22/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01 | |||||||||||
TATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTA > minE/2493606‑2493746 | tatCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCt > 1:2587836/1‑71 (MQ=255)tatCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCt < 1:3694531/71‑1 (MQ=255) tCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCtt < 1:3356194/70‑1 (MQ=255) gATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCa < 1:3012516/71‑1 (MQ=255) tttAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAAt < 1:2921712/69‑1 (MQ=255) tAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTa < 1:359626/70‑1 (MQ=255) gcTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGc < 1:3775165/57‑1 (MQ=255) gcTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAAttt < 1:2201371/71‑1 (MQ=255) gCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg > 1:177543/1‑69 (MQ=255) gCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTa < 1:928018/71‑1 (MQ=255) aatGCCTGATGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAg > 1:2701367/3‑71 (MQ=14) atGCCTGATGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAgg < 1:1678206/70‑1 (MQ=14) tGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa > 1:2386982/1‑52 (MQ=255) tGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa > 1:6608/1‑52 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCtt > 1:2278556/1‑38 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCg > 1:741336/1‑71 (MQ=255) cTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAAtttt < 1:1089963/53‑1 (MQ=255) cTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCg > 1:229203/1‑70 (MQ=255) gATGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGa > 1:277434/1‑70 (MQ=14) aTGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGAt < 1:1366568/70‑1 (MQ=14) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt > 1:1751399/1‑70 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt < 1:1127597/70‑1 (MQ=255) tGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAGTGTATTGAATTTGCACGATTTTGTAGGCCGGAt > 1:3531496/1‑69 (MQ=14) tGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt > 1:3830590/1‑69 (MQ=255) cgcTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAg < 1:1473834/70‑1 (MQ=14) cgcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAAttt < 1:318566/46‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAAttt < 1:3251488/46‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAgg < 1:1981069/71‑1 (MQ=255) gcTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAAtttg < 1:2458354/46‑2 (MQ=14) gcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAtttt < 1:2128235/55‑1 (MQ=255) gcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg > 1:2761066/1‑56 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAt < 1:1559875/51‑1 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAt < 1:2565870/51‑1 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAt < 1:1709508/51‑1 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCg < 1:3240778/62‑1 (MQ=255) tACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGt > 1:1674431/1‑71 (MQ=255) cGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGa < 1:632998/37‑1 (MQ=17) cGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGtt > 1:981727/1‑70 (MQ=14) cTTATCAGGCCTACGAAAATTCTGCAATGTATTGa > 1:3149691/1‑35 (MQ=14) cTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAc < 1:3193602/71‑1 (MQ=255) tATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:354434/48‑1 (MQ=255) tATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAcgc > 1:3533810/1‑71 (MQ=255) tCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTa > 1:1000507/1‑48 (MQ=255) ggCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGTTAACGCCGCAt < 1:1782256/71‑1 (MQ=14) ggCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:3135301/71‑1 (MQ=255) ggCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt > 1:3576027/1‑71 (MQ=255) gCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGTTAACGCCGCATc < 1:3501910/71‑1 (MQ=11) gCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:1685497/71‑1 (MQ=255) gCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTAACGCCGCATc < 1:893433/71‑1 (MQ=255) ccTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGTTAACGCCGCATcc < 1:1653805/71‑1 (MQ=11) cTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTa > 1:3602198/1‑42 (MQ=14) tACGAAAATCTTGCAATGTATTGAATTTTCATGAtttt < 1:258896/38‑1 (MQ=255) aCGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg > 1:3912605/1‑70 (MQ=255) gAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:989866/36‑1 (MQ=255) gAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATaa < 1:3297536/48‑1 (MQ=255) gAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa < 1:1248346/71‑1 (MQ=255) aaaTCTTGCAATGTATTGAATTTTCATGATTTTGTa > 1:1134761/1‑36 (MQ=255) aaaTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaag < 1:1423851/70‑1 (MQ=255) aaTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATa < 1:3883058/44‑1 (MQ=255) aTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaagaag > 1:1047723/1‑71 (MQ=255) tCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGc > 1:1307273/1‑71 (MQ=255) ttGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCta > 1:1279665/1‑71 (MQ=255) ttGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCt > 1:1898855/1‑70 (MQ=255) | TATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTA > minE/2493606‑2493746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |