| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,493,839 | A→C | 42.0% | intergenic (+221/‑154) | gyrB → / → ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,493,839 | 0 | A | C | 42.0% | ‑3.1 / 39.2 | 31 | intergenic (+221/‑154) | gyrB/ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Reads supporting (aligned to +/- strand): ref base A (10/8); new base C (8/5); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCCTTTTTATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGAT > minE/2493771‑2493905 | cGCCCTTTTTATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGaa > 1:2567523/1‑70 (MQ=255) tttATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGaaaaaaa < 1:2347191/68‑1 (MQ=255) cAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTg < 1:1244286/68‑1 (MQ=255) aTCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGaaaaaata > 1:898010/1‑55 (MQ=255) ccGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTcc < 1:3851612/60‑1 (MQ=255) cGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCt > 1:1795689/1‑71 (MQ=255) cAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAAGTGAAAAAAAAAGTCCGCTg < 1:3527744/61‑1 (MQ=255) atcatcCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCGGCTGATAAGGCTTGAAAAg > 1:1188538/1‑71 (MQ=255) atcatcCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAg > 1:138067/1‑71 (MQ=255) atcatcCCCCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAg > 1:1885073/1‑71 (MQ=255) atcCCGCAAATCTACTATGCAATAGACCATAAACTGa < 1:2395583/37‑1 (MQ=255) ccGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCAt > 1:2458817/1‑70 (MQ=255) ctaTGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGAc < 1:1224153/67‑1 (MQ=255) tGCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGaaa > 1:2284503/1‑49 (MQ=255) gCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCAt < 1:2447549/55‑1 (MQ=255) cAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGaa > 1:3222247/1‑46 (MQ=255) cAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATaa > 1:428101/1‑38 (MQ=255) cAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATa > 1:4577/1‑37 (MQ=255) aaTAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTAc < 1:314213/71‑1 (MQ=255) tAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACAt > 1:3174523/1‑71 (MQ=255) aGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATc > 1:1653941/1‑71 (MQ=255) aCCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCg > 1:2827357/1‑70 (MQ=255) aCCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGt < 1:1974917/71‑1 (MQ=255) ccATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCg > 1:3567961/1‑69 (MQ=255) cATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGa > 1:853118/1‑53 (MQ=255) cATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCa > 1:2191080/1‑51 (MQ=255) cATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAg > 1:2900946/1‑71 (MQ=255) aTAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGc < 1:2815515/71‑1 (MQ=255) tAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTAc < 1:1235998/62‑1 (MQ=255) tAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGcc < 1:3748904/71‑1 (MQ=255) tAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGc > 1:1507795/1‑70 (MQ=255) tAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGc > 1:648804/1‑70 (MQ=255) aaCTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCg < 1:425543/70‑1 (MQ=255) aaCTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCg < 1:514266/70‑1 (MQ=255) aCTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGAt < 1:915132/71‑1 (MQ=255) aaaaaaaaaGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGAt < 1:428783/67‑1 (MQ=255) | CGCCCTTTTTATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGAT > minE/2493771‑2493905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |