| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,494,512 | A→G | 7.4% | intergenic (+106/‑6) | ibpA → / → ibpB | heat shock chaperone/heat shock chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,494,512 | 0 | A | G | 7.4% | 226.3 / 7.5 | 81 | intergenic (+106/‑6) | ibpA/ibpB | heat shock chaperone/heat shock chaperone |
| Reads supporting (aligned to +/- strand): ref base A (42/33); new base G (3/3); total (45/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
TGCTCGCCGTCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTGGCCAACGC > minE/2494443‑2494582 | tGCTCGCCGTCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAAt > 1:2119663/1‑71 (MQ=255) gCCGTCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACt < 1:1707597/70‑1 (MQ=255) gCCGTCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTa > 1:2063649/1‑71 (MQ=255) gCCGTCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTa < 1:3193395/71‑1 (MQ=255) ccGTCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAGTGACTAt > 1:1305788/1‑71 (MQ=255) tCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCTCTTCTTAGAAGGAGAAATGACTATGCg > 1:1325762/1‑71 (MQ=255) tCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTATAAGGAGAAATGACTATGCg > 1:3478958/1‑71 (MQ=255) tCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCg > 1:2362154/1‑71 (MQ=255) tCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCg > 1:2584348/1‑71 (MQ=255) tCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCg > 1:760328/1‑71 (MQ=255) aGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGa > 1:3666472/1‑62 (MQ=255) aGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGt < 1:3917379/70‑1 (MQ=255) aGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGt < 1:2820033/70‑1 (MQ=255) aGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGt < 1:1832134/70‑1 (MQ=255) ggAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAAc < 1:1173884/71‑1 (MQ=255) gAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAAt < 1:12845/57‑1 (MQ=255) gCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTc < 1:1512655/71‑1 (MQ=255) cATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCg < 1:976580/71‑1 (MQ=255) atGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGa > 1:990624/1‑69 (MQ=255) atGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGAtt > 1:891573/1‑71 (MQ=255) tGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGAtt > 1:2881303/1‑70 (MQ=255) gCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTa > 1:3238165/1‑71 (MQ=255) cGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTa < 1:358241/70‑1 (MQ=255) cGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTAt > 1:1652131/1‑71 (MQ=255) gAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTa > 1:1888849/1‑52 (MQ=255) aaTCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGa < 1:2040807/64‑1 (MQ=255) aaTCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATcc > 1:2125858/1‑71 (MQ=255) aTCTTCGGATTTGCAGGTGCTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTAt > 1:3420534/1‑68 (MQ=255) aTCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTAt > 1:3712549/1‑68 (MQ=255) aTCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATccc < 1:1372530/71‑1 (MQ=255) tCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTa > 1:1279856/1‑49 (MQ=255) ttCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGa > 1:3327167/1‑44 (MQ=255) cGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGaaa > 1:2678744/1‑39 (MQ=255) cGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGaaa > 1:2736987/1‑39 (MQ=255) cGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATcccc < 1:2811281/67‑1 (MQ=255) cGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACt > 1:3261431/1‑70 (MQ=255) cGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACt < 1:2828619/70‑1 (MQ=255) aTTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACt > 1:627460/1‑51 (MQ=255) ttGCAGGTACTTACTCGCTTCTTAGAAGGAGAAGTGACTATGCGTAACTTCGATTTATCCCCACTGATGc > 1:16717/1‑70 (MQ=255) aGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCCCTGATGCGTCaa < 1:72917/71‑1 (MQ=255) gTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATg > 1:223037/1‑71 (MQ=255) tacttactCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATgg < 1:3045851/71‑1 (MQ=255) tacttactCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATgg < 1:2052169/71‑1 (MQ=255) acttactCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGa > 1:3537271/1‑71 (MQ=255) cttactCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATg > 1:2940976/1‑68 (MQ=255) ttactCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGAttt < 1:2278467/46‑1 (MQ=255) ttactCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGAt < 1:2751381/58‑1 (MQ=255) actCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGa > 1:3568137/1‑41 (MQ=255) actCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGa > 1:368564/1‑41 (MQ=255) actCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTAt > 1:1583712/1‑46 (MQ=255) actCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCgg > 1:2810794/1‑71 (MQ=255) tCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGAttt < 1:3109130/42‑1 (MQ=255) tCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACt < 1:2836264/51‑1 (MQ=255) gCTTCTTAGAAGGAGAAGTGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGtt < 1:1540883/69‑1 (MQ=255) gCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGtttt > 1:2696143/1‑71 (MQ=255) gCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGtttt < 1:1161369/71‑1 (MQ=255) gCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGtttt < 1:1130899/71‑1 (MQ=255) cttcttAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCaa > 1:1226756/1‑58 (MQ=255) ttcttAGAAGGAGAAGTGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATgg < 1:1761256/60‑1 (MQ=255) ttcttAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCAc > 1:1793955/1‑46 (MQ=255) ttcttAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGa > 1:3387132/1‑71 (MQ=255) ttcttAGAAGGAGAAATGACTATGAGTAACTTCGATTTATCCCCa > 1:167155/1‑45 (MQ=255) tcttAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATCCGTCAATGGATCGGTTTTGa > 1:2312946/1‑70 (MQ=255) cttAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCa > 1:806855/1‑54 (MQ=255) ttAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGt < 1:234656/63‑1 (MQ=255) ttAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGt < 1:2976043/63‑1 (MQ=255) ttAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGt < 1:3693833/63‑1 (MQ=255) aGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGa > 1:1607513/1‑56 (MQ=255) gAAGGAGAAGTGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACa < 1:2493123/67‑1 (MQ=255) gAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACaa < 1:3146274/68‑1 (MQ=255) gAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAAc < 1:3061622/70‑1 (MQ=255) aaGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTg < 1:3911412/71‑1 (MQ=255) aGGAGAAGTGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTgg > 1:672578/1‑71 (MQ=255) aGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTgg < 1:2506644/71‑1 (MQ=255) ggAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACa < 1:1512739/64‑1 (MQ=255) ggAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTgg > 1:299768/1‑70 (MQ=255) ggAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTGGc < 1:669752/71‑1 (MQ=255) agaAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTGGcc > 1:3793462/1‑70 (MQ=255) gaAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGtttt > 1:265440/1‑57 (MQ=255) gaAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACa < 1:1022949/61‑1 (MQ=255) gaAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTGGcc > 1:1724057/1‑69 (MQ=255) gaAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTGGCCaa > 1:1940839/1‑71 (MQ=255) gaAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTGGCCaa > 1:2231287/1‑71 (MQ=255) aTGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACaaa > 1:14686/1‑60 (MQ=255) aTGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTgg > 1:773289/1‑64 (MQ=255) aTGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTGGCCAACg > 1:578867/1‑70 (MQ=255) aTGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTGGCCAACGc < 1:1694447/71‑1 (MQ=255) aTGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTGGCCAACGc < 1:3125373/71‑1 (MQ=255) | TGCTCGCCGTCAGGGAGCATATGCGAATCTTCGGATTTGCAGGTACTTACTCGCTTCTTAGAAGGAGAAATGACTATGCGTAACTTCGATTTATCCCCACTGATGCGTCAATGGATCGGTTTTGACAAACTGGCCAACGC > minE/2494443‑2494582 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |