| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,507,530 | G→A | 13.1% | P198P (CCG→CCA) | yicI → | predicted alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,507,530 | 0 | G | A | 13.1% | 158.9 / 12.3 | 61 | P198P (CCG→CCA) | yicI | predicted alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base G (29/24); new base A (4/4); total (33/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGTGGG > minE/2507465‑2507598 | cAGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTa > 1:1830116/1‑71 (MQ=255) gACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACa > 1:19659/1‑71 (MQ=255) gaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAAcc < 1:1673012/71‑1 (MQ=255) aCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCg > 1:3770262/1‑71 (MQ=255) ggAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTaa > 1:1550382/1‑64 (MQ=255) ggAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTa > 1:2749756/1‑63 (MQ=255) ggAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGt > 1:1198779/1‑71 (MQ=255) ggAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCAtt > 1:550607/1‑52 (MQ=255) ccGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTa < 1:3395604/69‑1 (MQ=255) ccGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATg < 1:771164/71‑1 (MQ=255) cGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATgg > 1:1070326/1‑71 (MQ=255) cGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATgg > 1:2583280/1‑71 (MQ=255) cGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATgg > 1:207777/1‑71 (MQ=255) gggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTa < 1:1656687/49‑1 (MQ=255) gggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTa < 1:67742/49‑1 (MQ=255) gACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGt < 1:3566746/71‑1 (MQ=255) aCGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTa < 1:2859974/71‑1 (MQ=255) cggcggAACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTAc < 1:826651/71‑1 (MQ=255) cggcACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTAc > 1:2652255/1‑68 (MQ=255) acaAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCaa < 1:2808359/71‑1 (MQ=255) acaAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTgg > 1:3543238/1‑67 (MQ=255) aaGTACTGGACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAat > 1:3085507/1‑70 (MQ=255) aaGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAat > 1:385255/1‑70 (MQ=255) aaGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGt < 1:1529933/60‑1 (MQ=255) aaGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatc < 1:3318776/71‑1 (MQ=255) cTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATccc < 1:1112079/71‑1 (MQ=255) tGAACAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTa < 1:3414713/48‑1 (MQ=255) gAACAGGCGTATAAAAATATCCGGTTCTACATGACTAACCGTGGTTATg < 1:778421/49‑1 (MQ=255) gAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGt > 1:3678021/1‑59 (MQ=255) aaCAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTg < 1:3073620/56‑1 (MQ=255) aaCAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTg < 1:3193039/56‑1 (MQ=255) aaCAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTg < 1:1525552/56‑1 (MQ=255) aaCAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAg > 1:1942221/1‑71 (MQ=255) aaCAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAg > 1:3215584/1‑71 (MQ=255) aaCAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAg > 1:1447434/1‑71 (MQ=255) cAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAgtg > 1:38349/1‑71 (MQ=255) cAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAgtg > 1:3887555/1‑71 (MQ=255) cAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAgtg > 1:60231/1‑71 (MQ=255) cAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAgtg > 1:2279987/1‑71 (MQ=255) aGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAg > 1:322360/1‑68 (MQ=255) ggCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAgtgt < 1:1116744/70‑1 (MQ=255) gTATAAAAATATCCCATTCTACATGACTAACCGTGGtt > 1:2532668/1‑38 (MQ=255) tataAAAATATCCCGTTCTACATGACTAACCGTCGTTATGGGGTACTGGTCAATCATCCCCAGTGTGtctc < 1:1000515/71‑1 (MQ=255) ataAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTctct > 1:2136915/1‑71 (MQ=255) ataAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTctct > 1:766891/1‑71 (MQ=255) taAAAATATCCCGTTCTACATGACTAACCGTGGTTa > 1:2752612/1‑36 (MQ=255) taAAAATATCCCGTTCTACATGACTAACCGTGGTTa > 1:935913/1‑36 (MQ=255) taAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTctct < 1:298671/70‑1 (MQ=255) aaaaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAat < 1:3877409/51‑1 (MQ=255) aaaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtttt > 1:3683763/1‑71 (MQ=255) aaaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCttt < 1:1846123/70‑1 (MQ=255) aaaaTATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCttt < 1:3514730/70‑1 (MQ=255) aaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTg > 1:1492582/1‑70 (MQ=255) atatCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGaa < 1:961817/71‑1 (MQ=255) tatCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAg < 1:3078695/71‑1 (MQ=255) tCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGTg > 1:2075390/1‑71 (MQ=255) tCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGTg > 1:3511623/1‑71 (MQ=255) tCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCAAGTGTGTCTCTTTTGAAGTg > 1:2750039/1‑71 (MQ=255) ccGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGTggg < 1:3595895/71‑1 (MQ=255) cGTTCTACATGACTAACCGTGGTTATGGGGTACTg < 1:1885506/35‑1 (MQ=255) cGTTCTACATGACTAACCGTGGTTATGGGGTACTg < 1:3172396/35‑1 (MQ=255) | CAGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGTGGG > minE/2507465‑2507598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |