| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,547,976 | T→C | 24.6% | V62A (GTT→GCT) | htrL → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,547,976 | 0 | T | C | 24.6% | 114.4 / 40.4 | 69 | V62A (GTT→GCT) | htrL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (29/23); new base C (6/11); total (35/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01 | |||||||||||
ACTTTGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGATATATC > minE/2547907‑2548046 | aCTTTGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGt < 1:1326383/70‑1 (MQ=255) cTTTGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGtt < 1:2640431/70‑1 (MQ=255) cTTTGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGtt < 1:2846730/70‑1 (MQ=255) cTTTGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTg < 1:2807373/71‑1 (MQ=255) cTTTGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTg < 1:454972/71‑1 (MQ=255) tttGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTg > 1:2289769/1‑70 (MQ=255) tGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTg < 1:294537/68‑1 (MQ=255) tGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAg > 1:1117403/1‑71 (MQ=255) tGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTGa < 1:139424/69‑1 (MQ=255) cGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGAt < 1:3876381/71‑1 (MQ=255) cGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTGAAGCGAt < 1:809705/71‑1 (MQ=255) gTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTGa < 1:1466962/64‑1 (MQ=255) gTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTGa < 1:3807866/64‑1 (MQ=255) tCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTc > 1:1694977/1‑71 (MQ=255) cTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTGAAg > 1:3326733/1‑64 (MQ=255) tGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGa > 1:2887786/1‑66 (MQ=255) gCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCaa > 1:3347494/1‑71 (MQ=255) aCTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGaa < 1:1828544/71‑1 (MQ=255) aCTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGa > 1:3886053/1‑70 (MQ=255) cTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCg > 1:3799387/1‑57 (MQ=255) cTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGa > 1:3740408/1‑58 (MQ=255) cTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACgg < 1:3279026/68‑1 (MQ=255) cTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGaa < 1:3401834/70‑1 (MQ=255) gAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGAt > 1:2083114/1‑56 (MQ=255) aaaaTGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAAcc < 1:1326830/70‑1 (MQ=255) aaaTGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTGAAGCGATTCGCAACGGAAAACcaa > 1:1504287/1‑71 (MQ=255) aaTGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACcaa > 1:1523512/1‑70 (MQ=255) aTGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACcaaca < 1:621564/71‑1 (MQ=255) tGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACcaacaa < 1:2657499/71‑1 (MQ=255) tGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTGAAGCGATTCGCaa > 1:1072089/1‑57 (MQ=255) tGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTGAAGCGATTCGCAACGGAAAACcaacaa < 1:1396979/71‑1 (MQ=255) agaTGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGTAGCGATTCGCAACGGAAAACCaacaac < 1:87451/70‑1 (MQ=255) tgatTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGa < 1:3920291/40‑1 (MQ=255) tgatTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGa < 1:3924701/40‑1 (MQ=255) attattTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCaa > 1:2784488/1‑50 (MQ=255) attattTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAAc > 1:3850083/1‑51 (MQ=255) attattTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAAc > 1:3204233/1‑51 (MQ=255) attTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGAtt > 1:1855687/1‑42 (MQ=255) tttCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGgttatcgtta > 1:1864818/1‑71 (MQ=255) ttCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGgttatcgtta < 1:1546665/70‑1 (MQ=255) cACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGgttatcgttatc > 1:3476885/1‑70 (MQ=255) aCTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGttatcgttatcg < 1:3168280/70‑1 (MQ=255) ccGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGgttatcgttat < 1:3181552/61‑1 (MQ=255) ccGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGgttatcgttat < 1:2984744/61‑1 (MQ=255) ccGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGgttatcgttat < 1:495000/61‑1 (MQ=255) ccGATTTGAAACCAAGGGCTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATaaaa < 1:1812268/71‑1 (MQ=255) cGATTTGAAACCAAGTGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATaaaaa < 1:1022145/71‑1 (MQ=255) gATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGat > 1:3021144/1‑63 (MQ=255) aTTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATaaaa > 1:408230/1‑68 (MQ=255) aTTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATaaaa > 1:1158664/1‑68 (MQ=255) aTTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATaaaa > 1:1126847/1‑68 (MQ=255) tttGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAg > 1:2391667/1‑70 (MQ=255) tttGAAACCAAGGGCTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAg > 1:2554307/1‑70 (MQ=255) tttGAAACCAAGGGCTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGa < 1:2904482/71‑1 (MQ=255) ttGAAACCAAGGGTTGAAGCGATTCGCAACGGaaa > 1:1197627/1‑35 (MQ=255) ttGAAACCAAGGGTTGAAGCGATTCGCAACGGaaa > 1:168144/1‑35 (MQ=255) tGAAACCAAGGGCTGAAGCGATTCGCAACGGAAAACcaa > 1:2167975/1‑39 (MQ=255) gAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATaaaa < 1:1788448/64‑1 (MQ=255) aCCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGata > 1:3716/1‑56 (MQ=255) ccAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAAtt > 1:683319/1‑68 (MQ=255) ccAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAg < 1:699777/71‑1 (MQ=255) cAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGatat > 1:3224637/1‑55 (MQ=255) cAAGGGCTGAAGCGATTCGCAACGGAAAACCAACAACGgttatcgt < 1:3549699/46‑1 (MQ=255) cAAGGGCTGAAGCGATTCGCAACGGAAAACCAACAACGgttatcgt < 1:3049943/46‑1 (MQ=255) cAAGGGCTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGa < 1:976444/71‑1 (MQ=255) aGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGata > 1:2302610/1‑52 (MQ=255) aGGGCTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGata < 1:2327579/71‑1 (MQ=255) gggTTGAAGCGATTCGCAACGGAAAACCAACAACGgttatcg > 1:2217382/1‑42 (MQ=255) gggTTGAAGCGATTCGCAACGGAAAACCAACAACGGGTATCGTTATCGATATAAAAAAGAAATTTAGatat < 1:3126475/71‑1 (MQ=255) ggTTGAAGCGATTCGCAACGGAAAACCAACAACGgttatcgtt > 1:2150753/1‑43 (MQ=255) ggTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGatata < 1:914000/71‑1 (MQ=255) gTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGatatat > 1:3271999/1‑71 (MQ=255) ttGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGATATATc < 1:1095208/71‑1 (MQ=255) | ACTTTGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGATATATC > minE/2547907‑2548046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |