| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,562 | C→T | 5.5% | Q437Q (CAG→CAA) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,562 | 0 | C | T | 5.5% | 232.0 / 2.3 | 73 | Q437Q (CAG→CAA) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (34/35); new base T (2/2); total (36/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGT > minE/311493‑311629 | gCCAAAGAAGGCCATCGGTACTAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:3610260/71‑1 (MQ=255)gCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:2487857/71‑1 (MQ=255) cgaagaatGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGc > 1:2403519/3‑71 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcc > 1:403912/1‑71 (MQ=255) aagaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGccc > 1:1450726/1‑71 (MQ=255) aagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCAt > 1:3391678/1‑71 (MQ=255) ggCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCg < 1:2045518/71‑1 (MQ=255) ccATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGat > 1:976105/1‑70 (MQ=255) tCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:1606618/57‑1 (MQ=255) tCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTa > 1:361136/1‑71 (MQ=255) cGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc > 1:905829/1‑71 (MQ=255) cGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc > 1:901156/1‑71 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc < 1:2640374/70‑1 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc < 1:2000163/70‑1 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg > 1:3086170/1‑71 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg > 1:2299603/1‑71 (MQ=255) tACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACggg < 1:1733761/71‑1 (MQ=255) cGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGa > 1:709963/1‑61 (MQ=255) cGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACggg > 1:3645596/1‑69 (MQ=255) cGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGt < 1:611625/70‑1 (MQ=255) tACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGctt < 1:1490520/71‑1 (MQ=255) aCCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGAttttt < 1:3933907/61‑3 (MQ=255) ccgccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttct < 1:3703952/71‑1 (MQ=255) ccgccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttc < 1:1845213/70‑1 (MQ=255) ccgACAGTACGATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACtt < 1:2372600/55‑1 (MQ=255) ccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:293599/42‑1 (MQ=255) ccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt > 1:3561011/1‑71 (MQ=255) ccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt > 1:117307/1‑71 (MQ=255) ccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt > 1:3026723/1‑71 (MQ=255) cgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACtt < 1:1856760/54‑1 (MQ=255) cgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTg < 1:1364338/71‑1 (MQ=255) gACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACgg > 1:209257/1‑57 (MQ=255) gACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTgg < 1:161871/71‑1 (MQ=255) gTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcccc > 1:307287/1‑41 (MQ=255) gTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGa < 1:350186/46‑1 (MQ=255) gTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGa > 1:1192713/1‑46 (MQ=255) tACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTggcggc > 1:2325397/1‑70 (MQ=255) tACCATCGCGATACCGACAAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCa < 1:2533555/71‑1 (MQ=255) caCCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCg < 1:3603010/43‑1 (MQ=19) ccATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTggcg > 1:411462/1‑66 (MQ=255) aTCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAAcc < 1:1496248/71‑1 (MQ=255) aTCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAAcc > 1:3429363/1‑71 (MQ=255) tCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt < 1:1957728/59‑1 (MQ=255) tCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACct > 1:962547/1‑71 (MQ=255) ctcgATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACTGGt < 1:3882380/46‑1 (MQ=25) gcgATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACctt < 1:2469835/70‑1 (MQ=255) gATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTggc < 1:2242494/58‑1 (MQ=255) aTACCGACCAGAGCGCCCTGAATCTGCCCCATCGACtt < 1:2647006/38‑1 (MQ=255) aTACCGACCAGAGCGCCCTGAATCTGCCCCATCGACtt < 1:3570617/38‑1 (MQ=255) aTACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCttcttc < 1:3357675/71‑1 (MQ=255) tACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAAc > 1:2301690/1‑63 (MQ=255) tACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACcttc < 1:881262/67‑1 (MQ=255) aCCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCttt > 1:395997/1‑51 (MQ=255) cGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGcc < 1:457362/71‑1 (MQ=255) gACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc > 1:3423337/1‑35 (MQ=255) gACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGctt > 1:1352780/1‑44 (MQ=255) aCCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCa > 1:1969391/1‑70 (MQ=255) ccAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCAt > 1:62895/1‑70 (MQ=255) cAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATaa > 1:3859148/1‑71 (MQ=255) cAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATa > 1:3353268/1‑70 (MQ=255) agCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGcc < 1:3562030/64‑1 (MQ=255) agCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAaca < 1:2813696/70‑1 (MQ=255) gcgcCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAac > 1:2955526/1‑68 (MQ=255) cTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAAcc > 1:2382027/1‑48 (MQ=255) gAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATaa > 1:3338048/1‑60 (MQ=255) gAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACg > 1:2754914/1‑71 (MQ=255) aaTCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAAc > 1:2255476/1‑44 (MQ=255) aaTCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAAc > 1:3438977/1‑44 (MQ=255) aaTCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGt < 1:3380971/71‑1 (MQ=255) tCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACcttctt < 1:1294603/48‑1 (MQ=255) tCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACcttctt < 1:3934355/48‑1 (MQ=255) tCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACcttctt < 1:2294619/48‑1 (MQ=255) tCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACcttctt < 1:2865319/48‑1 (MQ=255) | GCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGT > minE/311493‑311629 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |