BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,540	G→T	7.9%	L513M (CTG→ATG)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,540	0	G	T	7.9%	316.9 / 8.7	100	L513M (CTG→ATG)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (44/48); new base T (5/3); total (49/51)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01

Only 100 of 105 total aligned reads displayed.

CCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGG  >  minE/2562470‑2562608

cccACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAg                                                                      <  1:3193289/71‑1 (MQ=255)

cccACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAg                                                                      >  1:3500627/1‑71 (MQ=255)

 ccACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAgg                                                                     >  1:2767826/1‑71 (MQ=255)

  cACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGc                                                                    <  1:3432462/71‑1 (MQ=255)

   aCTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGcc                                                                   >  1:3784197/1‑71 (MQ=255)

      ataCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                                >  1:2615783/1‑71 (MQ=255)

       taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTa                                                                 <  1:686596/69‑1 (MQ=255)

        aCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                              >  1:1007048/1‑71 (MQ=255)

        aCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                              >  1:2074836/1‑71 (MQ=255)

        aCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                              >  1:3516763/1‑71 (MQ=255)

         cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgc                                                             <  1:704610/71‑1 (MQ=255)

           ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                           <  1:3027271/71‑1 (MQ=255)

           ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                           >  1:1728095/1‑71 (MQ=255)

            gTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                           >  1:2058136/1‑70 (MQ=255)

            gTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                           >  1:2053432/1‑70 (MQ=255)

            gTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                          <  1:3802873/71‑1 (MQ=255)

             tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                         <  1:2385219/71‑1 (MQ=255)

               aaGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcaca                                                       <  1:3539052/71‑1 (MQ=255)

               aaGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                         >  1:2012181/1‑69 (MQ=255)

                aGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                      >  1:2659662/1‑71 (MQ=255)

                   tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTa                                                                 >  1:3914120/1‑57 (MQ=255)

                    cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                  >  1:3612896/1‑71 (MQ=255)

                    cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                  >  1:2126940/1‑71 (MQ=255)

                           tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAgga                                                                    >  1:1698809/1‑45 (MQ=255)

                           tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGc                                                                    >  1:3091210/1‑46 (MQ=255)

                           tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                            <  1:2106502/70‑1 (MQ=255)

                           tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcg                                             <  1:1385297/69‑1 (MQ=255)

                           tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcg                                             <  1:3718222/69‑1 (MQ=255)

                             tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCt                                                                  <  1:3379248/46‑1 (MQ=255)

                               tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGa                                        <  1:900050/70‑1 (MQ=255)

                                  cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                                    <  1:2649008/71‑1 (MQ=255)

                                  cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                                    >  1:2347841/1‑71 (MQ=255)

                                   aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                   >  1:563070/1‑71 (MQ=255)

                                    gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                  >  1:3663710/1‑71 (MQ=255)

                                    gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                  <  1:2653374/71‑1 (MQ=255)

                                    gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                  >  1:2822104/1‑71 (MQ=255)

                                    gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCgg                                  >  1:2226946/1‑71 (MQ=255)

                                     tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                                >  1:3280909/1‑40 (MQ=255)

                                     tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                 <  1:701858/71‑1 (MQ=255)

                                       aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCt                                                                  >  1:136735/1‑36 (MQ=255)

                                       aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                          <  1:2055413/60‑1 (MQ=255)

                                       aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgggg                                <  1:3463994/70‑1 (MQ=255)

                                       aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                 >  1:2317926/1‑69 (MQ=255)

                                       aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGga                               <  1:278935/71‑1 (MQ=255)

                                        aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                         <  1:264072/44‑1 (MQ=255)

                                        aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                 <  1:873430/52‑1 (MQ=255)

                                        aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                 <  1:321257/52‑1 (MQ=255)

                                         gCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                 >  1:206254/1‑67 (MQ=255)

                                          cGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGat                            <  1:576580/71‑1 (MQ=255)

                                           ggAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGat                            >  1:138170/1‑70 (MQ=255)

                                           ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                            >  1:2284432/1‑70 (MQ=255)

                                            gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                          <  1:2599496/71‑1 (MQ=255)

                                            gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                          <  1:1998823/71‑1 (MQ=255)

                                             aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                 <  1:3593175/47‑1 (MQ=255)

                                             aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                 <  1:1911568/47‑1 (MQ=255)

                                             aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                 <  1:2883883/47‑1 (MQ=255)

                                             aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCACACAGGCGATAGCGATAGATTGCgggg                                <  1:784215/64‑1 (MQ=255)

                                              aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                 >  1:1191154/1‑46 (MQ=255)

                                              aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                        >  1:3279114/1‑71 (MQ=255)

                                              aCAAATCAGACACTTTGCCCACCAGGCCTACCGCCGCACAGgcgatag                                               >  1:1720285/1‑48 (MQ=255)

                                               cAAATCAGACTCTTTGCACACCAGGACTACCGCCGCACAGgcgatag                                               >  1:15815/1‑47 (MQ=255)

                                                 aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                   <  1:2597273/57‑1 (MQ=255)

                                                 aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                     <  1:389391/71‑1 (MQ=255)

                                                  ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                    <  1:809536/70‑3 (MQ=255)

                                                  aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                      >  1:1068239/1‑69 (MQ=255)

                                                   tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgat                                           <  1:245443/47‑1 (MQ=255)

                                                   tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc                   <  1:3769706/71‑1 (MQ=255)

                                                    cAGACTCTTTGCCCACCAGGCCTACCGCCGCACAgg                                                     >  1:1395249/1‑36 (MQ=255)

                                                    cAGACTCTTTGCCCACCAGGCCTACCGCCGCACAgg                                                     >  1:1715191/1‑36 (MQ=255)

                                                    cAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgata                                                >  1:1267607/1‑41 (MQ=255)

                                                     aGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCg                  <  1:1210235/70‑1 (MQ=255)

                                                      gACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt                <  1:232027/71‑1 (MQ=255)

                                                       aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                     >  1:3142298/1‑65 (MQ=255)

                                                       aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                     >  1:2317194/1‑65 (MQ=255)

                                                       aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTg               <  1:2736074/71‑1 (MQ=255)

                                                       aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTg               <  1:2459157/71‑1 (MQ=255)

                                                        ctctTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                          >  1:672374/1‑43 (MQ=255)

                                                         tctTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                  <  1:676199/50‑1 (MQ=255)

                                                           tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcat                         >  1:3173815/1‑57 (MQ=255)

                                                           tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcat                         <  1:3885581/57‑1 (MQ=255)

                                                           tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc           >  1:677748/1‑71 (MQ=255)

                                                           tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc           >  1:2717157/1‑71 (MQ=255)

                                                           tcggCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGc           >  1:421311/4‑71 (MQ=255)

                                                             tGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCa         >  1:1028580/1‑71 (MQ=255)

                                                             ggCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccg         >  1:3720287/2‑70 (MQ=255)

                                                              gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGtta              >  1:1182852/1‑63 (MQ=255)

                                                              gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGcc          <  1:679284/69‑1 (MQ=255)

                                                               cccACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAcc       <  1:2061269/71‑1 (MQ=255)

                                                                ccaccaTGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccgccg      >  1:159661/1‑71 (MQ=255)

                                                                ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg      <  1:1039501/71‑1 (MQ=255)

                                                                ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg      <  1:2547204/71‑1 (MQ=255)

                                                                 caccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCgg     <  1:659663/71‑1 (MQ=255)

                                                                 caccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCgg     <  1:732329/71‑1 (MQ=255)

                                                                   ccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                      <  1:1725721/52‑1 (MQ=255)

                                                                    caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg  >  1:3293905/1‑71 (MQ=255)

                                                                    caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg  <  1:2312697/71‑1 (MQ=255)

                                                                    caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTCACCGGTGGCGTCACCggtaa  <  1:2461919/71‑3 (MQ=38)

                                                                     aGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                       >  1:3529145/1‑49 (MQ=255)

                                                                     aGGCCTACAGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                      >  1:1639258/1‑50 (MQ=255)

                                                                      ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgaga                             <  1:2008618/42‑1 (MQ=255)

CCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGG  >  minE/2562470‑2562608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`