Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,562,545 A→G 8.6% V511A (GTA→GCA) ‡ lldP ← L‑lactate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,562,5450AG 8.6% 260.6 / 12.2 93V511A (GTA→GCA) ‡lldPL‑lactate permease
Reads supporting (aligned to +/- strand):  ref base A (40/45);  new base G (5/3);  total (45/48)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

ATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGC  >  minE/2562476‑2562615
                                                                     |                                                                      
ataCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                                       >  1:2615783/1‑71 (MQ=255)
ataCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                                       >  1:3796546/1‑71 (MQ=255)
 taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTa                                                                        <  1:686596/69‑1 (MQ=255)
  aCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                                     >  1:1007048/1‑71 (MQ=255)
  aCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                                     >  1:2074836/1‑71 (MQ=255)
  aCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                                     >  1:3516763/1‑71 (MQ=255)
   cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgc                                                                    <  1:704610/71‑1 (MQ=255)
     ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                                  <  1:3027271/71‑1 (MQ=255)
     ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                                  >  1:1728095/1‑71 (MQ=255)
      gTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                                  >  1:2053432/1‑70 (MQ=255)
      gTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                                  >  1:2058136/1‑70 (MQ=255)
      gTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                                 <  1:3802873/71‑1 (MQ=255)
       tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                <  1:2385219/71‑1 (MQ=255)
         aaGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcaca                                                              <  1:3539052/71‑1 (MQ=255)
         aaGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                >  1:2012181/1‑69 (MQ=255)
          aGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                             >  1:2659662/1‑71 (MQ=255)
             tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTa                                                                        >  1:3914120/1‑57 (MQ=255)
              cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                         >  1:3612896/1‑71 (MQ=255)
              cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                         >  1:2126940/1‑71 (MQ=255)
                     tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                   <  1:2106502/70‑1 (MQ=255)
                     tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcg                                                    <  1:3718222/69‑1 (MQ=255)
                     tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcg                                                    <  1:1385297/69‑1 (MQ=255)
                         tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGa                                               <  1:900050/70‑1 (MQ=255)
                            cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAcc                                                                      <  1:1854585/44‑1 (MQ=255)
                            cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                                           <  1:2649008/71‑1 (MQ=255)
                            cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                                           >  1:2347841/1‑71 (MQ=255)
                             aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                          >  1:563070/1‑71 (MQ=255)
                              gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                         <  1:2653374/71‑1 (MQ=255)
                              gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                         >  1:2822104/1‑71 (MQ=255)
                              gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                         >  1:3663710/1‑71 (MQ=255)
                              gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCgg                                         >  1:2226946/1‑71 (MQ=255)
                               tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                                       >  1:3280909/1‑40 (MQ=255)
                               tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                        <  1:701858/71‑1 (MQ=255)
                                 aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                 <  1:2055413/60‑1 (MQ=255)
                                 aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgggg                                       <  1:3463994/70‑1 (MQ=255)
                                 aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                        >  1:2317926/1‑69 (MQ=255)
                                 aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGga                                      <  1:278935/71‑1 (MQ=255)
                                  aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                <  1:264072/44‑1 (MQ=255)
                                  aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                        <  1:321257/52‑1 (MQ=255)
                                  aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                        <  1:873430/52‑1 (MQ=255)
                                   gCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                        >  1:206254/1‑67 (MQ=255)
                                    cGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGat                                   <  1:576580/71‑1 (MQ=255)
                                    cGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                           >  1:919084/1‑47 (MQ=255)
                                     ggAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGat                                   >  1:138170/1‑70 (MQ=255)
                                     ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                   >  1:2284432/1‑70 (MQ=255)
                                      gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                                 <  1:2599496/71‑1 (MQ=255)
                                      gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                                 <  1:1998823/71‑1 (MQ=255)
                                       aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                        <  1:3593175/47‑1 (MQ=255)
                                       aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                        <  1:1911568/47‑1 (MQ=255)
                                       aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                        <  1:2883883/47‑1 (MQ=255)
                                       aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCACACAGGCGATAGCGATAGATTGCgggg                                       <  1:784215/64‑1 (MQ=255)
                                        aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                        >  1:1191154/1‑46 (MQ=255)
                                        aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                               >  1:3279114/1‑71 (MQ=255)
                                        aCAAATCAGACACTTTGCCCACCAGGCCTACCGCCGCACAGgcgatag                                                      >  1:1720285/1‑48 (MQ=255)
                                         cAAATCAGACTCTTTGCACACCAGGACTACCGCCGCACAGgcgatag                                                      >  1:15815/1‑47 (MQ=255)
                                           aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                          <  1:2597273/57‑1 (MQ=255)
                                           aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                            <  1:389391/71‑1 (MQ=255)
                                            ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                           <  1:809536/70‑3 (MQ=255)
                                            aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                             >  1:1068239/1‑69 (MQ=255)
                                             tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgat                                                  <  1:245443/47‑1 (MQ=255)
                                             tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc                          <  1:3769706/71‑1 (MQ=255)
                                              cAGACTCTTTGCCCACCAGGCCTACCGCCGCACAgg                                                            >  1:1395249/1‑36 (MQ=255)
                                              cAGACTCTTTGCCCACCAGGCCTACCGCCGCACAgg                                                            >  1:1715191/1‑36 (MQ=255)
                                              cAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgata                                                       >  1:1267607/1‑41 (MQ=255)
                                               aGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCg                         <  1:1210235/70‑1 (MQ=255)
                                                gACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt                       <  1:232027/71‑1 (MQ=255)
                                                 aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                            >  1:3142298/1‑65 (MQ=255)
                                                 aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                            >  1:2317194/1‑65 (MQ=255)
                                                 aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTg                      <  1:2444038/71‑1 (MQ=255)
                                                 aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTg                      <  1:2736074/71‑1 (MQ=255)
                                                 aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTg                      <  1:2459157/71‑1 (MQ=255)
                                                  ctctTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                 >  1:672374/1‑43 (MQ=255)
                                                   tctTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                         <  1:676199/50‑1 (MQ=255)
                                                     tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcat                                <  1:3885581/57‑1 (MQ=255)
                                                     tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcat                                >  1:3173815/1‑57 (MQ=255)
                                                     tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                  >  1:677748/1‑71 (MQ=255)
                                                     tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                  >  1:2717157/1‑71 (MQ=255)
                                                     tcggCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGc                  >  1:421311/4‑71 (MQ=255)
                                                       tGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCa                >  1:1028580/1‑71 (MQ=255)
                                                       ggCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccg                >  1:3720287/2‑70 (MQ=255)
                                                        gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGtta                     >  1:1182852/1‑63 (MQ=255)
                                                        gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGcc                 <  1:679284/69‑1 (MQ=255)
                                                         cccACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAcc              <  1:2061269/71‑1 (MQ=255)
                                                          ccaccaTGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccgccg             >  1:159661/1‑71 (MQ=255)
                                                          ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg             <  1:1039501/71‑1 (MQ=255)
                                                          ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg             <  1:2547204/71‑1 (MQ=255)
                                                           caccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCgg            <  1:659663/71‑1 (MQ=255)
                                                           caccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCgg            <  1:732329/71‑1 (MQ=255)
                                                             ccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                             <  1:1725721/52‑1 (MQ=255)
                                                              caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg         >  1:3293905/1‑71 (MQ=255)
                                                              caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg         <  1:2312697/71‑1 (MQ=255)
                                                              caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTCACCGGTGGCGTCACCggtaa         <  1:2461919/71‑3 (MQ=38)
                                                               aGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                              >  1:3529145/1‑49 (MQ=255)
                                                               aGGCCTACAGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                             >  1:1639258/1‑50 (MQ=255)
                                                                ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgaga                                    <  1:2008618/42‑1 (MQ=255)
                                                                     aCCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggc  >  1:1933971/1‑71 (MQ=255)
                                                                     |                                                                      
ATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGC  >  minE/2562476‑2562615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑