BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,559	G→C	11.1%	I506M (ATC→ATG) ‡	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,559	0	G	C	11.1%	218.9 / 15.3	81	I506M (ATC→ATG) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (40/32); new base C (5/4); total (45/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

Only 100 of 102 total aligned reads displayed.

CAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAA  >  minE/2562490‑2562627

cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                                     >  1:3612896/1‑71 (MQ=255)

cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                                     >  1:2126940/1‑71 (MQ=255)

       tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                               <  1:2106502/70‑1 (MQ=255)

       tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcg                                                                <  1:3718222/69‑1 (MQ=255)

       tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcg                                                                <  1:1385297/69‑1 (MQ=255)

              cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                                                       <  1:2649008/71‑1 (MQ=255)

              cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                                                       >  1:2347841/1‑71 (MQ=255)

               aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                                      >  1:563070/1‑71 (MQ=255)

                gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                                     >  1:3663710/1‑71 (MQ=255)

                gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                                     <  1:2653374/71‑1 (MQ=255)

                gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                                     >  1:2822104/1‑71 (MQ=255)

                gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCgg                                                     >  1:2226946/1‑71 (MQ=255)

                 tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                                    <  1:701858/71‑1 (MQ=255)

                   aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                             <  1:2055413/60‑1 (MQ=255)

                   aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgggg                                                   <  1:3463994/70‑1 (MQ=255)

                   aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                                    >  1:2317926/1‑69 (MQ=255)

                   aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGga                                                  <  1:278935/71‑1 (MQ=255)

                    aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                                    <  1:873430/52‑1 (MQ=255)

                    aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                                    <  1:321257/52‑1 (MQ=255)

                     gCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                                    >  1:206254/1‑67 (MQ=255)

                      cGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGat                                               <  1:576580/71‑1 (MQ=255)

                       ggAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGat                                               >  1:138170/1‑70 (MQ=255)

                       ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                               >  1:2284432/1‑70 (MQ=255)

                        gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                                             <  1:1998823/71‑1 (MQ=255)

                        gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                                             <  1:2599496/71‑1 (MQ=255)

                         aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                                    <  1:2883883/47‑1 (MQ=255)

                         aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                                    <  1:1911568/47‑1 (MQ=255)

                         aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                                    <  1:3593175/47‑1 (MQ=255)

                         aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCACACAGGCGATAGCGATAGATTGCgggg                                                   <  1:784215/64‑1 (MQ=255)

                          aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                                    >  1:1191154/1‑46 (MQ=255)

                          aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                                           >  1:3279114/1‑71 (MQ=255)

                          aCAAATCAGACACTTTGCCCACCAGGCCTACCGCCGCACAGgcgatag                                                                  >  1:1720285/1‑48 (MQ=255)

                           cAAATCAGACTCTTTGCACACCAGGACTACCGCCGCACAGgcgatag                                                                  >  1:15815/1‑47 (MQ=255)

                             aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                                      <  1:2597273/57‑1 (MQ=255)

                             aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                                        <  1:389391/71‑1 (MQ=255)

                              ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                                       <  1:809536/70‑3 (MQ=255)

                              aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                         >  1:1068239/1‑69 (MQ=255)

                               tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgat                                                              <  1:245443/47‑1 (MQ=255)

                               tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc                                      <  1:3769706/71‑1 (MQ=255)

                                cAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgata                                                                   >  1:1267607/1‑41 (MQ=255)

                                 aGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCg                                     <  1:1210235/70‑1 (MQ=255)

                                  gACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt                                   <  1:232027/71‑1 (MQ=255)

                                   aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                                        >  1:3142298/1‑65 (MQ=255)

                                   aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                                        >  1:2317194/1‑65 (MQ=255)

                                   aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTg                                  <  1:2459157/71‑1 (MQ=255)

                                   aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTg                                  <  1:2444038/71‑1 (MQ=255)

                                   aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTg                                  <  1:2736074/71‑1 (MQ=255)

                                    ctctTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                             >  1:672374/1‑43 (MQ=255)

                                     tctTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                                     <  1:676199/50‑1 (MQ=255)

                                       tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcat                                            <  1:3885581/57‑1 (MQ=255)

                                       tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcat                                            >  1:3173815/1‑57 (MQ=255)

                                       tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                              >  1:677748/1‑71 (MQ=255)

                                       tcggCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGc                              >  1:421311/4‑71 (MQ=255)

                                         tGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCa                            >  1:1028580/1‑71 (MQ=255)

                                         ggCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccg                            >  1:3720287/2‑70 (MQ=255)

                                          gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGtta                                 >  1:1182852/1‑63 (MQ=255)

                                          gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGcc                             <  1:679284/69‑1 (MQ=255)

                                           cccACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAcc                          <  1:2061269/71‑1 (MQ=255)

                                            ccaccaTGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccgccg                         >  1:159661/1‑71 (MQ=255)

                                            ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg                         <  1:1039501/71‑1 (MQ=255)

                                            ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg                         <  1:2547204/71‑1 (MQ=255)

                                             caccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCgg                        <  1:732329/71‑1 (MQ=255)

                                             caccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCgg                        <  1:659663/71‑1 (MQ=255)

                                               ccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                         <  1:1725721/52‑1 (MQ=255)

                                                caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg                     <  1:2312697/71‑1 (MQ=255)

                                                caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg                     >  1:3293905/1‑71 (MQ=255)

                                                caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTCACCGGTGGCGTCACCggtaa                     <  1:2461919/71‑3 (MQ=38)

                                                 aGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                                          >  1:3529145/1‑49 (MQ=255)

                                                 aGGCCTACAGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                         >  1:1639258/1‑50 (MQ=255)

                                                  ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgaga                                                <  1:2008618/42‑1 (MQ=255)

                                                       aCCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggc              >  1:1933971/1‑71 (MQ=255)

                                                        ccgccgCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggcg             >  1:464145/1‑71 (MQ=255)

                                                        ccgccgCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggcg             >  1:2456872/1‑71 (MQ=255)

                                                         cgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGccag                                    <  1:2784748/47‑3 (MQ=16)

                                                          gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                   >  1:1912159/1‑63 (MQ=255)

                                                          gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggcgg            >  1:3036914/1‑70 (MQ=255)

                                                           ccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt                                   >  1:1236178/1‑46 (MQ=255)

                                                           ccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggcggc           >  1:1666617/1‑70 (MQ=255)

                                                             gcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc        >  1:3799266/1‑71 (MQ=255)

                                                              cacaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc        >  1:2603306/1‑70 (MQ=255)

                                                              cacaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc        >  1:3749515/1‑70 (MQ=255)

                                                               acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg                     <  1:3764947/56‑1 (MQ=255)

                                                               acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc        >  1:2893795/1‑69 (MQ=255)

                                                               acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc        >  1:2224424/1‑69 (MQ=255)

                                                               acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc        >  1:3236348/1‑69 (MQ=255)

                                                               acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc        >  1:2817278/1‑69 (MQ=255)

                                                               acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCa      <  1:2730025/71‑1 (MQ=255)

                                                               acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCa      <  1:2734771/71‑1 (MQ=255)

                                                                 aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGAc                                >  1:787385/1‑43 (MQ=255)

                                                                 aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGAc                                >  1:3203072/1‑43 (MQ=255)

                                                                 aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                   >  1:27069/1‑56 (MQ=255)

                                                                 aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                   >  1:620107/1‑56 (MQ=255)

                                                                 aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAg     >  1:3752057/1‑70 (MQ=255)

                                                                 aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc    <  1:123004/71‑1 (MQ=255)

                                                                  ggCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAg     >  1:1264295/1‑69 (MQ=255)

                                                                  ggCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc    >  1:1758728/1‑70 (MQ=255)

                                                                   gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa  <  1:2127498/71‑1 (MQ=255)

                                                                    cgatagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa  >  1:3319831/1‑70 (MQ=255)

                                                                    cgatagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa  >  1:3345587/1‑70 (MQ=255)

                                                                     gatagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggcggc           <  1:2875224/60‑1 (MQ=255)

CAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAA  >  minE/2562490‑2562627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`