Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,733	G→A	8.5%	F448F (TTC→TTT)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,733	0	G	A	8.5%	243.7 / 8.7	82	F448F (TTC→TTT)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base G (38/37);  new base A (5/2);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCG  >  minE/2562663‑2562802                                                                       |                                                                      cTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCAAGCCAGCCGAgg                                                                       <  1:1715006/71‑1 (MQ=255)  ttGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAgg                                                                       >  1:1927720/1‑70 (MQ=255)       gcgcGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGc                                                                 <  1:1087162/71‑1 (MQ=255)            gcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaa                                                            <  1:1758913/71‑1 (MQ=255)               aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACggt                                                                 >  1:1096630/1‑62 (MQ=255)               aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACgg                                                                  >  1:2521122/1‑62 (MQ=255)               aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGagaag                                                           <  1:83398/69‑1 (MQ=255)                 cAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaa                                                            >  1:3873898/1‑66 (MQ=255)                 cAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaa                                                            >  1:3869225/1‑66 (MQ=255)                   gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGaa                                                                     >  1:3295031/1‑55 (MQ=255)                    ggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGa                                                    <  1:1327871/71‑1 (MQ=255)                    ggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGa                                                    <  1:124496/71‑1 (MQ=255)                      cGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAATGTGAAt                                                  <  1:505031/71‑1 (MQ=255)                      cGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                                                   >  1:3290278/1‑70 (MQ=255)                        ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGa                                                    <  1:1370431/67‑1 (MQ=255)                        ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt                                                  <  1:2929700/69‑1 (MQ=255)                        ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGc                                                <  1:2770260/71‑1 (MQ=255)                         tAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaacat                                                >  1:1457708/1‑67 (MQ=255)                          aGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCa                                               <  1:850830/70‑1 (MQ=255)                          aGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCAt                                              >  1:3828736/1‑71 (MQ=255)                           gATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCAt                                              <  1:995191/70‑1 (MQ=255)                            aggAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACg                                                                   <  1:1772796/46‑1 (MQ=19)                            aTGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGa                                            >  1:3147750/1‑71 (MQ=255)                            aTGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGa                                            >  1:1929560/1‑71 (MQ=255)                             tGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGa                                                                      >  1:2189709/1‑44 (MQ=255)                              gAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCgag                                                              <  1:1882487/51‑1 (MQ=255)                              gAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCgag                                                              <  1:3308563/51‑1 (MQ=255)                              gAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAcc                                          >  1:618523/1‑71 (MQ=255)                               aGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCg                                         <  1:2615893/71‑1 (MQ=255)                                ggTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAgg                                                       >  1:1576350/1‑56 (MQ=255)                                ggTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCgg                                        <  1:1222223/71‑1 (MQ=255)                                  tATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGgtg                                      <  1:721815/71‑1 (MQ=255)                                   aTCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATg                                             <  1:2799648/63‑1 (MQ=255)                                   aTCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGgtgt                                     >  1:3640938/1‑71 (MQ=255)                                    tCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaaga                                                          >  1:1271012/1‑49 (MQ=255)                                    tCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATg                                             <  1:1101573/62‑1 (MQ=255)                                     ccGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCAt                                              >  1:3584682/1‑60 (MQ=255)                                       gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                 <  1:1735141/71‑1 (MQ=255)                                        aCCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                 >  1:154673/1‑70 (MQ=255)                                         cccGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                               <  1:2371450/71‑1 (MQ=255)                                         cccGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                               <  1:2318651/71‑1 (MQ=255)                                            ggTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                             >  1:974396/1‑70 (MQ=255)                                            ggTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTg                            >  1:1907396/1‑71 (MQ=255)                                                  gAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                                                     <  1:1736152/40‑1 (MQ=255)                                                  gAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                                                     <  1:3160904/40‑1 (MQ=255)                                                  gAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCTGTGTGCGCCAGTGCCAgcg                      >  1:2761252/1‑71 (MQ=255)                                                  gAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcg                      >  1:1748500/1‑71 (MQ=255)                                                   aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                     >  1:3154040/1‑71 (MQ=255)                                                    aTACTCCCAGCCAGCCGAGAAACGGTGAgaagaac                                                        >  1:332702/1‑34 (MQ=25)                                                    aTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                     >  1:1022546/1‑70 (MQ=255)                                                    aTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                     >  1:1247580/1‑70 (MQ=255)                                                     tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                     >  1:2790105/1‑69 (MQ=255)                                                     tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCa                   <  1:925556/71‑1 (MQ=255)                                                       cccccAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                             <  1:2733568/59‑1 (MQ=255)                                                       cccccAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAgt                 <  1:2901893/71‑1 (MQ=255)                                                        ccccAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt                                                  <  1:1279357/37‑1 (MQ=255)                                                         cccAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAgtgt               >  1:661893/1‑71 (MQ=255)                                                         cccAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAgtgt               <  1:679790/71‑1 (MQ=255)                                                          ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGgtg                                      >  1:1106044/1‑47 (MQ=255)                                                          ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt              >  1:1472290/1‑71 (MQ=255)                                                           cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt              >  1:3657369/1‑70 (MQ=255)                                                           cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt              >  1:2741785/1‑70 (MQ=255)                                                           cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt              >  1:2600238/1‑70 (MQ=255)                                                           cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt              >  1:2404804/1‑70 (MQ=255)                                                           cagccagcCGAGGAAAGGCGAGAAGAAGGTGAATGCATGACCg                                         >  1:2072616/1‑43 (MQ=255)                                                            agccagccGAGGAACGGCGAGAAGAAGGTGAATGCa                                               >  1:1916934/1‑36 (MQ=255)                                                            agccagccGAGGAACGGCGAGAAGAAGGTGAATGCa                                               >  1:2186394/1‑36 (MQ=255)                                                            agccagccGAGGAACGGCGAGAAGAAGGTGAATGCa                                               >  1:3944/1‑36 (MQ=255)                                                              ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGgtgt                                     <  1:3798058/44‑1 (MQ=255)                                                              ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAg                              >  1:2814234/1‑51 (MQ=255)                                                               cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga         >  1:869274/1‑71 (MQ=255)                                                               cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga         <  1:3822908/71‑1 (MQ=255)                                                               cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga         <  1:530700/71‑1 (MQ=255)                                                               cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat           <  1:3447356/69‑1 (MQ=255)                                                                agccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtga            <  1:718904/67‑1 (MQ=255)                                                                agccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtga            <  1:1631394/67‑1 (MQ=255)                                                                 gccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACa       <  1:385335/71‑1 (MQ=255)                                                                  ccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAgtg                <  1:615884/61‑1 (MQ=255)                                                                   cGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtga            >  1:2449187/1‑64 (MQ=255)                                                                   cGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGt     >  1:2545349/1‑71 (MQ=255)                                                                   cGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGt     >  1:2901704/1‑71 (MQ=255)                                                                    gAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt              >  1:1059032/1‑61 (MQ=255)                                                                    gAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTc    <  1:2389987/71‑1 (MQ=255)                                                                    gAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTc    <  1:362665/71‑1 (MQ=255)                                                                      ggAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAg                  >  1:419162/1‑55 (MQ=255)                                                                      ggAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCg  <  1:2302443/71‑1 (MQ=255)                                                                      ggAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCCCCAGTGTTGATGAGAGTCCg  <  1:505821/71‑1 (MQ=255)                                                                       gAACGGCGAGAAGAAGGTGAATGCATGACCGgtgt                                     >  1:1414417/1‑35 (MQ=255)                                                                       |                                                                      CTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCG  >  minE/2562663‑2562802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑