Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,569,793 | A→G | 11.0% | G21G (GGA→GGG) | yibI → | predicted inner membrane protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,569,793 | 0 | A | G | 11.0% | 220.5 / 15.4 | 82 | G21G (GGA→GGG) | yibI | predicted inner membrane protein |
Reads supporting (aligned to +/- strand): ref base A (37/36); new base G (6/3); total (43/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01 |
AGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAC > minE/2569723‑2569856 | aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa < 1:3706631/71‑1 (MQ=255) aatatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat > 1:1099372/2‑71 (MQ=255) tatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcg > 1:239493/1‑71 (MQ=255) gTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:1036736/1‑71 (MQ=255) tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:3007544/68‑1 (MQ=255) tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:960544/68‑1 (MQ=255) tCTAAACTATTTCGCGCTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:1639939/66‑1 (MQ=255) tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt > 1:476931/1‑71 (MQ=255) aaaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa > 1:1031908/1‑69 (MQ=255) aaCTATTTCGCGTTGGTAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt < 1:2338748/69‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa < 1:1647543/68‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt < 1:2121342/69‑1 (MQ=255) aCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGAc < 1:3658050/71‑1 (MQ=255) tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc < 1:2773618/55‑1 (MQ=255) tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATcc > 1:3021482/1‑71 (MQ=255) tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATccc < 1:1708663/70‑1 (MQ=255) cgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTAt > 1:960427/1‑71 (MQ=255) ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt < 1:101214/67‑1 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:3114019/1‑70 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:587957/1‑70 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:894829/1‑70 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:1007652/1‑70 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACAt < 1:2919653/60‑1 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGAAATCATCGCGATACATGAc < 1:86651/58‑1 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa > 1:1890939/1‑53 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa > 1:1870730/1‑53 (MQ=255) gAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:467429/1‑70 (MQ=255) aGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTg < 1:3123798/71‑1 (MQ=255) gTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTg > 1:1295997/1‑70 (MQ=255) gTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTg > 1:1642966/1‑70 (MQ=255) gTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGc > 1:3934834/1‑71 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATc < 1:1674716/56‑1 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATAc > 1:2033589/1‑47 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:1467725/65‑1 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:1790345/1‑43 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa > 1:462838/1‑70 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa > 1:1497526/1‑70 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAtt > 1:3436943/1‑65 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt < 1:3059743/64‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa < 1:3897787/69‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa < 1:2674449/69‑1 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa > 1:2114060/1‑69 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgc > 1:2045046/1‑71 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:3757642/1‑70 (MQ=255) tCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt < 1:3266974/58‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:1502056/1‑54 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:3618881/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:2397959/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:995146/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:3864142/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:3868828/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:3941678/70‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:3363620/1‑67 (MQ=255) gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCaa < 1:478405/71‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:2798126/52‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa > 1:2025948/1‑69 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa > 1:798974/1‑71 (MQ=255) ttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaatc > 1:2529465/1‑67 (MQ=255) ttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat > 1:2307023/1‑67 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc < 1:1955310/68‑1 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCAt < 1:995434/70‑1 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc > 1:1074034/1‑71 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc > 1:2266703/1‑71 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGACAAAAAgcg > 1:3170979/1‑63 (MQ=255) cTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:2047506/1‑71 (MQ=255) tGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCaaaaa < 1:696601/58‑1 (MQ=255) ggTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAacgta < 1:1893992/62‑3 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa < 1:1866585/55‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCaa < 1:748539/62‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:1767787/69‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCa < 1:2789212/71‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCa > 1:2533292/1‑71 (MQ=255) gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATcct < 1:3379106/69‑2 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa < 1:3122978/52‑1 (MQ=255) aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:1876429/1‑44 (MQ=255) aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:3765969/1‑44 (MQ=255) ttttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc > 1:2100605/1‑71 (MQ=255) tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc > 1:2744494/1‑70 (MQ=255) tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc > 1:3350040/1‑70 (MQ=255) ttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCg < 1:249353/70‑1 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTg > 1:572273/1‑38 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc > 1:571351/1‑71 (MQ=255) ggAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa < 1:3220887/43‑1 (MQ=255) | AGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAC > minE/2569723‑2569856 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |