BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	132,196	T→C	10.0%	D163D (GAT→GAC)	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	132,196	0	T	C	10.0%	279.5 / 17.8	100	D163D (GAT→GAC)	yadD	predicted transposase
Reads supporting (aligned to +/- strand): ref base T (61/29); new base C (7/3); total (68/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 106 total aligned reads displayed.

TCGCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCG  >  minE/132128‑132263

tCGCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGa                                                                   >  1:3442030/1‑71 (MQ=255)

 cGCCGGAGCTGGCGCGACTCGTCTATAACAGTCCTTTCCCGCTGGTGGATATAACCATCACACCGGATg                                                                    >  1:3597756/1‑69 (MQ=255)

  gCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGAc                                                                  >  1:3197789/1‑70 (MQ=255)

  gCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGAc                                                                  >  1:1761879/1‑70 (MQ=255)

  gCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGAc                                                                  >  1:142356/1‑70 (MQ=255)

  gCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGAc                                                                  >  1:3504281/1‑70 (MQ=255)

   ccGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGa                                                                <  1:518685/71‑1 (MQ=255)

    cGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGa                                                                >  1:778636/1‑70 (MQ=255)

       aGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATc                                                            <  1:1731507/71‑1 (MQ=255)

        gCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCa                                                           >  1:2408109/1‑71 (MQ=255)

         cTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCAt                                                          <  1:2266054/71‑1 (MQ=255)

          tGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCTGAt                                                                     <  1:3322144/59‑1 (MQ=255)

          tGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGAt                                                                     <  1:2905069/59‑1 (MQ=255)

          tGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGAt                                                                     <  1:2069502/59‑1 (MQ=255)

          tGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGAt                                                                     <  1:1568648/59‑1 (MQ=255)

           ggCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACg                                                                 >  1:1192265/1‑62 (MQ=255)

           ggCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATg                                                         >  1:750181/1‑70 (MQ=255)

           ggCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATg                                                         >  1:643475/1‑70 (MQ=255)

            gcgcGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATg                                                         <  1:3744934/69‑1 (MQ=255)

            gcgcGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGc                                                        <  1:1973193/70‑1 (MQ=255)

            gcgcGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGc                                                        <  1:1923041/70‑1 (MQ=255)

            gcgcGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGc                                                        >  1:922081/1‑70 (MQ=255)

             cgcgACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATc                                                            >  1:3571361/1‑65 (MQ=255)

              gcgACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCaa                                                      >  1:1598612/1‑70 (MQ=255)

              gcgACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCaa                                                      >  1:2757408/1‑70 (MQ=255)

              gcgACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAAc                                                     <  1:746966/71‑1 (MQ=255)

              gcgACGCGTCTATAACAGTCCTTTCCCGATGGTGGATATCACCATCACACCGGATGACGAAATCATGCaa                                                      >  1:157050/1‑70 (MQ=255)

               cgACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACa                                                    >  1:729607/1‑71 (MQ=255)

               cgACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACa                                                    <  1:2993070/71‑1 (MQ=255)

               cgACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGAAGAAATCATGCAACa                                                    >  1:1576723/1‑71 (MQ=255)

                 aCGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATc                                                  >  1:3718495/1‑71 (MQ=255)

                   gcgTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGa                                                                   >  1:2926153/1‑52 (MQ=255)

                    cgTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATctgc                                               <  1:1326904/71‑1 (MQ=255)

                      tCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCAt                                                          <  1:2143457/58‑1 (MQ=255)

                       cTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGa                                            >  1:2375227/1‑71 (MQ=255)

                          taACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGc                                                        >  1:1131343/1‑56 (MQ=255)

                          taACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGc                                                        >  1:3311360/1‑56 (MQ=255)

                           aaCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc                                        >  1:1834634/1‑71 (MQ=255)

                            aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc                                        <  1:3546386/70‑1 (MQ=255)

                             cAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                      >  1:2340633/1‑71 (MQ=255)

                              aGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGAt                                     >  1:2146896/1‑71 (MQ=255)

                                tCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtc                                   <  1:2289256/71‑1 (MQ=255)

                                tCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtc                                   <  1:1865246/71‑1 (MQ=255)

                                  cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                 <  1:1654283/71‑1 (MQ=255)

                                  cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                 >  1:1154686/1‑71 (MQ=255)

                                  cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtct                                  >  1:2360845/1‑70 (MQ=255)

                                   tttCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATcggc                                               <  1:2305727/56‑1 (MQ=255)

                                     tCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                >  1:3672365/1‑69 (MQ=255)

                                      cccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                <  1:1911822/68‑1 (MQ=255)

                                        cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATg                                                         <  1:1888484/41‑1 (MQ=255)

                                        cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                           >  1:1559944/1‑71 (MQ=255)

                                         gCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactac                          >  1:3282126/1‑71 (MQ=255)

                                          cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCa                                                       >  1:1649488/1‑41 (MQ=255)

                                          atggtggATATCACCATCACACCGGATGACGAAATCATGCa                                                       >  1:3124053/2‑41 (MQ=255)

                                           tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                              >  1:2689060/1‑65 (MQ=255)

                                           tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                        >  1:1378704/1‑71 (MQ=255)

                                           tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                        >  1:3699296/1‑71 (MQ=255)

                                                gATATCACCATCGTACCTGACGATGAGATCATGCAACATcggcg                                              >  1:3257111/1‑44 (MQ=255)

                                                gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                    <  1:2961241/70‑1 (MQ=255)

                                                 atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                      >  1:788052/1‑51 (MQ=255)

                                                 atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                      >  1:597024/1‑51 (MQ=255)

                                                  tatCACCATCACACCGGATGACGAAATCATGCAAc                                                     >  1:113110/1‑35 (MQ=255)

                                                  tatCACCATCACACCGGATGACGAAATCATGCAACATcggc                                               >  1:2884706/1‑41 (MQ=255)

                                                   atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                >  1:524861/1‑71 (MQ=255)

                                                   atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                >  1:312576/1‑71 (MQ=255)

                                                   atCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                    <  1:232113/67‑1 (MQ=255)

                                                    tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                >  1:3279009/1‑70 (MQ=255)

                                                    tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                >  1:1589625/1‑70 (MQ=255)

                                                     cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                <  1:820672/53‑1 (MQ=255)

                                                      aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat             <  1:3378901/71‑1 (MQ=255)

                                                      aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTAGAact                            >  1:2996407/1‑56 (MQ=255)

                                                      aCCATCACACCGGATGACGAAATCATGCAACATcggcg                                              >  1:3106062/1‑38 (MQ=255)

                                                      aCCATCACACCGGATGACGAAATCATGCAACATcggcg                                              >  1:2493090/1‑38 (MQ=255)

                                                      aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat             <  1:1866093/71‑1 (MQ=255)

                                                       ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCaaaa                   >  1:501805/1‑64 (MQ=255)

                                                       ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat             <  1:895428/70‑1 (MQ=255)

                                                       ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat             >  1:369444/1‑70 (MQ=255)

                                                       ccATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATAtt            >  1:3018776/1‑71 (MQ=255)

                                                        cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc           >  1:3459369/1‑71 (MQ=255)

                                                        cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc           >  1:1094608/1‑71 (MQ=255)

                                                        aaTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc           >  1:2295944/2‑71 (MQ=255)

                                                         aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                         >  1:3632017/1‑40 (MQ=255)

                                                         aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                         >  1:3328833/1‑40 (MQ=255)

                                                         aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc           >  1:1704122/1‑70 (MQ=255)

                                                          tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtct                                  >  1:2133987/1‑46 (MQ=255)

                                                          tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                <  1:3474398/48‑1 (MQ=255)

                                                          tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata              >  1:413424/1‑66 (MQ=255)

                                                          tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                <  1:3129806/64‑1 (MQ=255)

                                                          tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc         <  1:822055/71‑1 (MQ=255)

                                                           cgtacCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGc         <  1:353302/67‑1 (MQ=255)

                                                           cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata              >  1:1445872/1‑65 (MQ=255)

                                                            acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                              >  1:1816522/1‑48 (MQ=255)

                                                            acacCGGATGACGAAATCATGCAACATCGGCGGATAGCGATTCTCg                                >  1:538351/1‑46 (MQ=255)

                                                             cacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc        <  1:1085283/69‑1 (MQ=255)

                                                              acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactact                         <  1:2242153/51‑1 (MQ=255)

                                                              acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc     >  1:3892751/1‑71 (MQ=255)

                                                              acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc     >  1:1215807/1‑71 (MQ=255)

                                                                cGGATGACGAAATCATGCAACATCGGCGGATTGCg                                       >  1:192327/1‑35 (MQ=255)

                                                                 ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGcgcg  <  1:1739920/71‑1 (MQ=255)

                                                                    tGACGAAATCATGCAACATCGGCGGATTGCGATtct                                  <  1:1927416/36‑1 (MQ=255)

TCGCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCG  >  minE/132128‑132263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`