| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,814 | C→T | 9.3% | I28I (ATC→ATT) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,814 | 0 | C | T | 9.3% | 248.7 / 11.9 | 86 | I28I (ATC→ATT) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (44/34); new base T (5/3); total (49/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
TTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCC > minE/2569745‑2569884 | tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATcc > 1:3021482/1‑71 (MQ=255) tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATccc < 1:1708663/70‑1 (MQ=255) cgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTAt > 1:960427/1‑71 (MQ=255) ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt < 1:101214/67‑1 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:1007652/1‑70 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:894829/1‑70 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:587957/1‑70 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:3114019/1‑70 (MQ=255) gAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:467429/1‑70 (MQ=255) aGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTg < 1:3123798/71‑1 (MQ=255) gTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTg > 1:1295997/1‑70 (MQ=255) gTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTg > 1:1642966/1‑70 (MQ=255) gTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGc > 1:3934834/1‑71 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATc < 1:1674716/56‑1 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:1467725/65‑1 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa > 1:462838/1‑70 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa > 1:1497526/1‑70 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAtt > 1:3436943/1‑65 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt < 1:3059743/64‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa < 1:2674449/69‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa < 1:3897787/69‑1 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa > 1:2114060/1‑69 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgc > 1:2045046/1‑71 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:3757642/1‑70 (MQ=255) tCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt < 1:3266974/58‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:1502056/1‑54 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:2397959/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:995146/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:3618881/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:3864142/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:3868828/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:3941678/70‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:3363620/1‑67 (MQ=255) gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCaa < 1:478405/71‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:2798126/52‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa > 1:2025948/1‑69 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa > 1:798974/1‑71 (MQ=255) ttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaatc > 1:2529465/1‑67 (MQ=255) ttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat > 1:2307023/1‑67 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc < 1:1955310/68‑1 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCAt < 1:995434/70‑1 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc > 1:2266703/1‑71 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc > 1:1074034/1‑71 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGACAAAAAgcg > 1:3170979/1‑63 (MQ=255) cTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:2047506/1‑71 (MQ=255) tGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCaaaaa < 1:696601/58‑1 (MQ=255) ggTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAacgta < 1:1893992/62‑3 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa < 1:1866585/55‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCaa < 1:748539/62‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:1767787/69‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCa < 1:2789212/71‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCa > 1:2533292/1‑71 (MQ=255) gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATcct < 1:3379106/69‑2 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa < 1:3122978/52‑1 (MQ=255) aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:3765969/1‑44 (MQ=255) aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:1876429/1‑44 (MQ=255) ttttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc > 1:2100605/1‑71 (MQ=255) tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc > 1:3350040/1‑70 (MQ=255) tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc > 1:2744494/1‑70 (MQ=255) ttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCg < 1:249353/70‑1 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTg > 1:572273/1‑38 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc > 1:571351/1‑71 (MQ=255) ggAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa < 1:3220887/43‑1 (MQ=255) atcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc < 1:1982544/70‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCaaaaa > 1:2465508/1‑40 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:1920899/1‑70 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:57758/1‑70 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:75986/1‑70 (MQ=255) gcgATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:1934114/1‑36 (MQ=255) gcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc > 1:3639745/1‑71 (MQ=255) cgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCACCCATCCCCATGCCGACGCTATTCATAcggag > 1:916638/1‑69 (MQ=255) cgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg < 1:1953051/71‑1 (MQ=255) aTACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt < 1:3783623/60‑1 (MQ=255) tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc < 1:1830793/42‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:3943071/1‑36 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:990938/1‑61 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:1921031/1‑61 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCt < 1:124014/70‑1 (MQ=255) tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:1094361/1‑50 (MQ=255) gACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt > 1:1269066/1‑71 (MQ=255) gACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt > 1:2138577/1‑71 (MQ=255) cttCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:2725533/3‑50 (MQ=255) cATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:276661/43‑1 (MQ=255) cATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt > 1:3620947/1‑69 (MQ=255) cATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGa < 1:1503116/71‑1 (MQ=255) cATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGa < 1:3176992/71‑1 (MQ=255) aTCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa < 1:663654/53‑1 (MQ=255) aTCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAg > 1:2305542/1‑71 (MQ=255) tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc < 1:149024/71‑1 (MQ=255) cccTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:1496976/1‑37 (MQ=255) cccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGcc > 1:843393/1‑71 (MQ=255) cccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGcc > 1:842159/1‑71 (MQ=255) cccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGcc > 1:3491670/1‑71 (MQ=255) | TTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCC > minE/2569745‑2569884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |