| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,867 | C→T | 19.5% | T46M (ACG→ATG) ‡ | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,867 | 0 | C | T | 19.5% | 167.5 / 32.1 | 77 | T46M (ACG→ATG) ‡ | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (38/24); new base T (8/7); total (46/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01 | |||||||||||
GCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTA > minE/2569800‑2569936 | gcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc > 1:3639745/1‑71 (MQ=255) cgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCACCCATCCCCATGCCGACGCTATTCATAcggag > 1:916638/1‑69 (MQ=255) cgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg < 1:1953051/71‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCt < 1:124014/70‑1 (MQ=255) gACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt > 1:2138577/1‑71 (MQ=255) gACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt > 1:1269066/1‑71 (MQ=255) cATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGt > 1:3620947/1‑69 (MQ=255) cATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGa < 1:3176992/71‑1 (MQ=255) cATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGa < 1:1503116/71‑1 (MQ=255) aTCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAg > 1:2305542/1‑71 (MQ=255) tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc < 1:149024/71‑1 (MQ=255) cccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGcc > 1:3491670/1‑71 (MQ=255) cccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGcc > 1:843393/1‑71 (MQ=255) cccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGcc > 1:842159/1‑71 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt < 1:1558395/71‑1 (MQ=255) taccTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt > 1:111463/4‑71 (MQ=255) tGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcg > 1:3510562/1‑70 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTa < 1:971577/67‑1 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActgt > 1:2019885/1‑69 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct > 1:3013801/1‑71 (MQ=255) tttccAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:3710796/4‑71 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcgg > 1:428812/1‑45 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcgg > 1:24152/1‑45 (MQ=255) tGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg < 1:524203/70‑1 (MQ=255) ccAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGc < 1:3466348/69‑1 (MQ=255) ccAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCAt > 1:3241722/1‑71 (MQ=255) aaaaaaCGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt > 1:2014367/1‑59 (MQ=255) aaaaaaCGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt > 1:3375704/1‑59 (MQ=255) aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt > 1:564705/1‑71 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATAcggc < 1:1183526/40‑1 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt > 1:937618/1‑70 (MQ=255) aaaaCGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt > 1:2508762/1‑57 (MQ=255) aaaGCGCAACCATCCCCATGCCGACGCTATTCATAcgg > 1:2275429/1‑38 (MQ=255) aaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTa > 1:3159904/1‑71 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTa > 1:2022477/1‑70 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt < 1:890090/71‑1 (MQ=255) aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc > 1:3358137/1‑71 (MQ=255) aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:985524/71‑1 (MQ=255) aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:1049032/71‑1 (MQ=255) gcgcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc > 1:2446425/1‑49 (MQ=255) gcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTg < 1:2839573/70‑1 (MQ=255) cAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGc < 1:1188419/71‑1 (MQ=255) atcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGcc < 1:425152/68‑1 (MQ=255) aCCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCg > 1:188367/1‑71 (MQ=255) ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt > 1:415982/1‑71 (MQ=255) cATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGtt > 1:948653/1‑71 (MQ=255) aTCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGttt > 1:2523582/1‑71 (MQ=255) aTCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGtt < 1:1663610/70‑1 (MQ=255) aTCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATATGGCCGtt < 1:2218898/70‑1 (MQ=255) tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc < 1:1351690/71‑1 (MQ=255) tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc < 1:2416691/71‑1 (MQ=255) ccATGCCGACGCTATTAATACGGCGGGCTGGGTGAGCCTGCTTACTCTGCATGtt < 1:2461933/55‑1 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg < 1:1107913/71‑1 (MQ=255) cATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCt > 1:1243315/1‑58 (MQ=255) aTGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:1085642/1‑55 (MQ=255) aTGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:3549510/1‑70 (MQ=255) tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct > 1:3891268/1‑45 (MQ=255) tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt > 1:1016108/1‑62 (MQ=255) tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg < 1:3709517/70‑1 (MQ=255) cGACGCTATTCATACGGCGGGCTGGGTGAGCCTGt > 1:377072/1‑35 (MQ=255) gACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgt < 1:2100924/64‑1 (MQ=255) gACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTg < 1:2600880/71‑1 (MQ=255) gACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTg < 1:611473/71‑1 (MQ=255) gACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTg < 1:3418814/71‑1 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:3411234/1‑41 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:2800461/1‑41 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGc < 1:2571869/42‑1 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGc < 1:1838883/42‑1 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTgg > 1:1384040/1‑71 (MQ=255) gCTATTCATACGGCGGGCTGGGTGAGCCTGTTTTCTCTGCATGTTATCt > 1:172294/1‑49 (MQ=255) tATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGAt > 1:3457178/1‑63 (MQ=255) tATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGAt > 1:3461707/1‑63 (MQ=255) aTTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa > 1:3498529/1‑70 (MQ=255) ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACg > 1:3337287/1‑71 (MQ=255) ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACg > 1:1590569/1‑71 (MQ=255) ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATCTTAt > 1:3624033/1‑43 (MQ=255) tCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc < 1:137562/48‑1 (MQ=255) cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:3509457/1‑41 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGc < 1:3270038/64‑1 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc < 1:381442/71‑1 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:3766557/1‑71 (MQ=255) tACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctct < 1:3087948/71‑1 (MQ=255) aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctct > 1:3399315/1‑70 (MQ=255) aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTa > 1:2907231/1‑71 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:840233/1‑68 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:1410986/1‑68 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:3786883/1‑68 (MQ=255) | GCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTA > minE/2569800‑2569936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |