| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,892 | T→G | 18.7% | T54T (ACT→ACG) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,892 | 0 | T | G | 18.7% | 158.5 / 45.3 | 91 | T54T (ACT→ACG) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (39/35); new base G (7/10); total (46/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATG > minE/2569824‑2569961 | aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActgt > 1:2019885/1‑69 (MQ=255)aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct > 1:3013801/1‑71 (MQ=255) tttccAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:3710796/4‑71 (MQ=255) tGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg < 1:524203/70‑1 (MQ=255) ccAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGc < 1:3466348/69‑1 (MQ=255) ccAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCAt > 1:3241722/1‑71 (MQ=255) aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt > 1:564705/1‑71 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt > 1:937618/1‑70 (MQ=255) aaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTa > 1:3159904/1‑71 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTa > 1:2022477/1‑70 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt < 1:890090/71‑1 (MQ=255) aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:1049032/71‑1 (MQ=255) aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:985524/71‑1 (MQ=255) aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc > 1:3358137/1‑71 (MQ=255) gcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTg < 1:2839573/70‑1 (MQ=255) cAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGc < 1:1188419/71‑1 (MQ=255) atcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGcc < 1:425152/68‑1 (MQ=255) aCCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCg > 1:188367/1‑71 (MQ=255) ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt > 1:415982/1‑71 (MQ=255) cATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGtt > 1:948653/1‑71 (MQ=255) aTCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGttt > 1:2523582/1‑71 (MQ=255) aTCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGtt < 1:1663610/70‑1 (MQ=255) aTCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATATGGCCGtt < 1:2218898/70‑1 (MQ=255) tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc < 1:1351690/71‑1 (MQ=255) tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc < 1:2416691/71‑1 (MQ=255) ccATGCCGACGCTATTAATACGGCGGGCTGGGTGAGCCTGCTTACTCTGCATGtt < 1:2461933/55‑1 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg < 1:1107913/71‑1 (MQ=255) cATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCt > 1:1243315/1‑58 (MQ=255) aTGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:1085642/1‑55 (MQ=255) aTGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:3549510/1‑70 (MQ=255) tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct > 1:3891268/1‑45 (MQ=255) tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt > 1:1016108/1‑62 (MQ=255) tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg < 1:3709517/70‑1 (MQ=255) gACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgt < 1:2100924/64‑1 (MQ=255) gACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTg < 1:611473/71‑1 (MQ=255) gACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTg < 1:3418814/71‑1 (MQ=255) gACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTg < 1:2600880/71‑1 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:3411234/1‑41 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:2800461/1‑41 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGc < 1:2571869/42‑1 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGc < 1:1838883/42‑1 (MQ=255) aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTgg > 1:1384040/1‑71 (MQ=255) gCTATTCATACGGCGGGCTGGGTGAGCCTGTTTTCTCTGCATGTTATCt > 1:172294/1‑49 (MQ=255) tATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGAt > 1:3457178/1‑63 (MQ=255) tATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGAt > 1:3461707/1‑63 (MQ=255) aTTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa > 1:3498529/1‑70 (MQ=255) ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACg > 1:1590569/1‑71 (MQ=255) ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACg > 1:3337287/1‑71 (MQ=255) ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATCTTAt > 1:3624033/1‑43 (MQ=255) tCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc < 1:137562/48‑1 (MQ=255) cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:3509457/1‑41 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGc < 1:3270038/64‑1 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:3766557/1‑71 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc < 1:381442/71‑1 (MQ=255) tACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctct < 1:3087948/71‑1 (MQ=255) aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctct > 1:3399315/1‑70 (MQ=255) aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTa > 1:2907231/1‑71 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:1410986/1‑68 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:840233/1‑68 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:3786883/1‑68 (MQ=255) ggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:186579/1‑36 (MQ=255) gcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg > 1:3936005/1‑51 (MQ=255) gcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCa < 1:2277035/71‑1 (MQ=255) cggGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:783882/35‑1 (MQ=255) gggCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgt < 1:3629224/47‑1 (MQ=255) ggCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAAcc < 1:1521807/71‑1 (MQ=255) gttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTa > 1:1399135/3‑64 (MQ=255) tGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTgg > 1:2417065/1‑45 (MQ=255) tGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTa > 1:935435/1‑62 (MQ=255) tGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCg > 1:2309242/1‑69 (MQ=255) gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt < 1:3578078/40‑1 (MQ=255) gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt < 1:3725711/40‑1 (MQ=255) gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt < 1:1408607/40‑1 (MQ=255) gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt < 1:2314624/40‑1 (MQ=255) gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg < 1:727625/49‑1 (MQ=255) ggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAg < 1:1096122/70‑1 (MQ=255) ggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGc > 1:619740/1‑71 (MQ=255) gTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCg < 1:791423/71‑1 (MQ=255) tGAGTCTTTTTACGTTACATGTTATCTGGCCGttt < 1:3703128/35‑1 (MQ=16) tGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtg < 1:3322792/38‑1 (MQ=255) tGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTAt > 1:1754319/1‑59 (MQ=255) tGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGt > 1:767200/1‑71 (MQ=255) gAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg > 1:2382118/1‑39 (MQ=255) gAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGa < 1:3575894/41‑1 (MQ=255) aGCCTGTTTACTCTGCTTGTTATCTGGCCGTTTCt < 1:2753530/35‑1 (MQ=255) aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTgg > 1:3481944/1‑71 (MQ=255) aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTgg > 1:2586938/1‑71 (MQ=255) cTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTAt > 1:2444902/1‑54 (MQ=255) gTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTa > 1:2757702/1‑51 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTTTGGATCTGGGCGACGCTCTATCAACCGGa < 1:2788372/59‑1 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCaa < 1:3919277/54‑1 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgg < 1:2841122/69‑1 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGCCGACGCTCTATCAACCGGAGCGTGGCTgg < 1:3581725/69‑1 (MQ=255) aCTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTa < 1:3701735/70‑1 (MQ=255) ctctGCATGTTATCTGGCCTTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTAt < 1:1233029/70‑1 (MQ=255) tctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc < 1:618787/43‑1 (MQ=255) tctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCa < 1:1926712/48‑1 (MQ=255) tctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg < 1:1138669/65‑1 (MQ=255) tctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATg > 1:3079914/1‑70 (MQ=255) | ATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATG > minE/2569824‑2569961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |