| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,928 | G→C | 10.0% | A66A (GCG→GCC) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,928 | 0 | G | C | 10.0% | 193.0 / 10.1 | 70 | A66A (GCG→GCC) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (33/30); new base C (3/4); total (36/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
ATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAGCGACTGACC > minE/2569860‑2569998 | aTTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa > 1:3498529/1‑70 (MQ=255) ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACg > 1:3337287/1‑71 (MQ=255) ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACg > 1:1590569/1‑71 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc < 1:381442/71‑1 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:3766557/1‑71 (MQ=255) tACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctct < 1:3087948/71‑1 (MQ=255) aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctct > 1:3399315/1‑70 (MQ=255) aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTa > 1:2907231/1‑71 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:840233/1‑68 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:3786883/1‑68 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc > 1:1410986/1‑68 (MQ=255) gcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCa < 1:2277035/71‑1 (MQ=255) ggCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAAcc < 1:1521807/71‑1 (MQ=255) gttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTa > 1:1399135/3‑64 (MQ=255) tGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTa > 1:935435/1‑62 (MQ=255) tGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCg > 1:2309242/1‑69 (MQ=255) ggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAg < 1:1096122/70‑1 (MQ=255) ggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGc > 1:619740/1‑71 (MQ=255) gTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCg < 1:791423/71‑1 (MQ=255) tGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTAt > 1:1754319/1‑59 (MQ=255) tGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGt > 1:767200/1‑71 (MQ=255) aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTgg > 1:3481944/1‑71 (MQ=255) aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTgg > 1:2586938/1‑71 (MQ=255) cTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTAt > 1:2444902/1‑54 (MQ=255) gTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTa > 1:2757702/1‑51 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTTTGGATCTGGGCGACGCTCTATCAACCGGa < 1:2788372/59‑1 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCaa < 1:3919277/54‑1 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgg < 1:2841122/69‑1 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGCCGACGCTCTATCAACCGGAGCGTGGCTgg < 1:3581725/69‑1 (MQ=255) aCTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTa < 1:3701735/70‑1 (MQ=255) ctctGCATGTTATCTGGCCTTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTAt < 1:1233029/70‑1 (MQ=255) tctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc < 1:618787/43‑1 (MQ=255) tctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCa < 1:1926712/48‑1 (MQ=255) tctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg < 1:1138669/65‑1 (MQ=255) tctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATg > 1:3079914/1‑70 (MQ=255) ttacATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCGGGGATGGGGAATGCa < 1:429336/68‑1 (MQ=255) ctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg > 1:468772/1‑64 (MQ=255) ctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg > 1:1977202/1‑64 (MQ=255) ctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTAt < 1:40474/68‑1 (MQ=255) tGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgggg > 1:2762863/1‑64 (MQ=255) tGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGt > 1:3541952/1‑65 (MQ=255) tGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGt > 1:3665235/1‑65 (MQ=255) tGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAg > 1:3877369/1‑71 (MQ=255) gCATGTCATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGt > 1:513138/1‑71 (MQ=255) acATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCGGGGATGGGGAATGCaa < 1:3222498/69‑2 (MQ=255) cATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAg < 1:2177747/69‑1 (MQ=255) cATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTc < 1:490593/71‑1 (MQ=255) cATGTTATCTGGCCGTTTCTGTGGATCTGGGCCACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAg < 1:1728481/69‑1 (MQ=255) tGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATTCAGTcac < 1:532216/71‑1 (MQ=255) gTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCt < 1:838650/55‑1 (MQ=255) ttATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTcaca < 1:2107844/70‑1 (MQ=255) tATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTa > 1:2957339/1‑36 (MQ=19) tATCTGGCCGTTTCTGTGGATCTGGGCGACGCACTa > 1:549532/1‑36 (MQ=255) tCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTg < 1:2409752/52‑1 (MQ=255) cTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg > 1:1266829/1‑53 (MQ=255) tGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg > 1:1344991/1‑52 (MQ=255) gCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCg < 1:308330/70‑1 (MQ=255) cGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCg > 1:2185154/1‑71 (MQ=255) tttCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGt < 1:2909974/62‑1 (MQ=255) tttCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGc < 1:2352216/65‑1 (MQ=255) tttCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGc < 1:110587/65‑1 (MQ=255) tCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAg < 1:2412101/70‑1 (MQ=255) tCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAg < 1:2190818/70‑1 (MQ=255) gATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg < 1:3112459/53‑1 (MQ=255) gATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGaaa < 1:1331031/70‑1 (MQ=255) gATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAg > 1:3090772/1‑71 (MQ=255) gATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAg > 1:1316728/1‑71 (MQ=255) cTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAGCGa > 1:2259260/1‑71 (MQ=255) gCGACGCTCTATCAACCGGAGCGTGGCTGGGGTAt > 1:2439121/1‑35 (MQ=255) gCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAGCGACt < 1:1651485/69‑1 (MQ=255) gACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAGCGACTGAcc > 1:3051070/1‑71 (MQ=255) gACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAGCGACTGAcc < 1:1208264/71‑1 (MQ=255) | ATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAGCGACTGACC > minE/2569860‑2569998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |