BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,570,774	A→G	6.8%	N227D (AAT→GAT)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,570,774	0	A	G	6.8%	289.6 / 8.5	102	N227D (AAT→GAT)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (48/47); new base G (5/2); total (53/49)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TGAGCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGA  >  minE/2570706‑2570843

tgAGCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAAt                                                                     <  1:3222161/71‑1 (MQ=255)

  aGCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATgg                                                                   >  1:1037541/1‑71 (MQ=255)

   gCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCaa                                                                      >  1:2221381/1‑67 (MQ=255)

      tAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTAc                                                               >  1:2667099/1‑71 (MQ=255)

       aaGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACg                                                              >  1:367369/1‑71 (MQ=255)

         gagCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGt                                                             >  1:2672745/1‑70 (MQ=255)

          agCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                           >  1:95976/1‑71 (MQ=255)

           gcgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAAt                                                                     >  1:1909125/1‑60 (MQ=255)

           gcgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAAt                                                                     >  1:1913732/1‑60 (MQ=255)

            cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                         <  1:643878/71‑1 (MQ=255)

            cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                         <  1:2765956/71‑1 (MQ=255)

             gcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                        <  1:457105/71‑1 (MQ=255)

              cAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                        <  1:1830841/70‑1 (MQ=255)

              cAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                       <  1:2194854/71‑1 (MQ=255)

               aaCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGt                                                             <  1:783101/64‑1 (MQ=255)

               aaCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                      <  1:50951/71‑1 (MQ=255)

                aCTTACTGAAGCTAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAgg                                                     >  1:3601015/1‑71 (MQ=255)

                aCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                           >  1:896908/1‑65 (MQ=255)

                aCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                       <  1:3559689/69‑1 (MQ=255)

                  ttACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                   >  1:1399077/1‑71 (MQ=255)

                   tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                  >  1:1029350/1‑71 (MQ=255)

                   tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                  >  1:1561352/1‑71 (MQ=255)

                   tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                  >  1:1409704/1‑71 (MQ=255)

                    gcggAAGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACt                                                 <  1:2379157/68‑1 (MQ=255)

                     cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                 <  1:2908671/70‑1 (MQ=255)

                     cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                 <  1:1591148/70‑1 (MQ=255)

                     cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                 >  1:236619/1‑70 (MQ=255)

                      tGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                               >  1:46339/1‑71 (MQ=255)

                      tGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                               >  1:2540261/1‑71 (MQ=255)

                      ggAAGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGa                                               >  1:2949883/2‑71 (MQ=255)

                       gAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                 <  1:1056164/68‑1 (MQ=255)

                       gAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCCAGCAATGGCt                                                                 <  1:2213941/52‑1 (MQ=255)

                        aaGCAAAATATAACCTTTAGCAGACTGTCATTCGCGCGCCGAGCAATgg                                                                   >  1:3524585/1‑49 (MQ=255)

                        aaGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGAt                                              >  1:2247349/1‑70 (MQ=255)

                        aaGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                             >  1:1762874/1‑71 (MQ=255)

                        aaGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGAtt                                             >  1:2765907/1‑70 (MQ=255)

                         aGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATcc                                            >  1:1456328/1‑71 (MQ=255)

                         aGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTat                                                               >  1:1921474/1‑51 (MQ=255)

                          gaaaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                        <  1:1554036/56‑1 (MQ=255)

                          gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCaa                                                                      >  1:1233979/1‑44 (MQ=255)

                          gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCg                                           >  1:1420436/1‑71 (MQ=255)

                           cAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGc                                          >  1:1267847/1‑71 (MQ=255)

                            aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCt                                                                 <  1:76420/47‑1 (MQ=255)

                            aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                      >  1:203839/1‑58 (MQ=255)

                            aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                      >  1:2551507/1‑58 (MQ=255)

                            aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                      >  1:3827925/1‑58 (MQ=255)

                            aaaaTATAACCTTCAGCAGACTGTCATTCGCGCGCCGAGCa                                                                       <  1:2016311/41‑1 (MQ=255)

                             aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                       >  1:193785/1‑56 (MQ=255)

                             aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                        >  1:2760089/1‑71 (MQ=255)

                             aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                        >  1:687261/1‑71 (MQ=255)

                                  taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                       <  1:2179532/51‑1 (MQ=255)

                                  taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                    <  1:228450/70‑1 (MQ=255)

                                  taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                   <  1:2530590/71‑1 (MQ=255)

                                   atccTTGAGGAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                        <  1:26174/47‑1 (MQ=255)

                                   aaCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCt                                                                 <  1:1226409/40‑1 (MQ=255)

                                    aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGAt                                              >  1:1236603/1‑58 (MQ=255)

                                    aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                 <  1:597022/71‑1 (MQ=255)

                                    aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                 <  1:172741/71‑1 (MQ=255)

                                     ccTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                   <  1:3372093/52‑1 (MQ=255)

                                      cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCt                                                                 <  1:1013344/37‑1 (MQ=255)

                                      cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                >  1:3429211/1‑70 (MQ=255)

                                      cTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTa                               <  1:605910/71‑1 (MQ=255)

                                         gAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACg                             <  1:219833/70‑1 (MQ=255)

                                          aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGTTACATACGCa                           <  1:124614/71‑1 (MQ=255)

                                          aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATAc                              <  1:2264375/68‑1 (MQ=255)

                                           gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                          <  1:1923624/71‑1 (MQ=255)

                                           gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                          >  1:3662023/1‑71 (MQ=255)

                                            cAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                          <  1:2766676/70‑1 (MQ=255)

                                              gACTGTAATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                   >  1:1486909/1‑59 (MQ=255)

                                               aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                   <  1:2367045/42‑1 (MQ=255)

                                               aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                      >  1:2567420/1‑55 (MQ=255)

                                                cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGAt                                              >  1:3114791/1‑46 (MQ=255)

                                                cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGcc                     <  1:2710821/71‑1 (MQ=255)

                                                   tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                    >  1:3534992/1‑53 (MQ=255)

                                                   tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTg                  >  1:2686476/1‑71 (MQ=255)

                                                     aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                >  1:3900848/1‑71 (MQ=255)

                                                      ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg               <  1:1976035/71‑1 (MQ=255)

                                                      ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg               <  1:2242318/71‑1 (MQ=255)

                                                      ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg               >  1:1142127/1‑71 (MQ=255)

                                                      ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg               >  1:735532/1‑71 (MQ=255)

                                                      ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg               <  1:3869112/71‑1 (MQ=255)

                                                       tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                               >  1:523345/1‑38 (MQ=255)

                                                       tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                               >  1:3128848/1‑38 (MQ=255)

                                                       tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                               >  1:124238/1‑38 (MQ=255)

                                                       tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                               >  1:3298004/1‑38 (MQ=255)

                                                        cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt             >  1:3179459/1‑71 (MQ=255)

                                                        cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt             >  1:3438704/1‑71 (MQ=255)

                                                         gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt             <  1:664843/70‑1 (MQ=255)

                                                         gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt             <  1:481804/70‑1 (MQ=255)

                                                           gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg          <  1:43089/71‑1 (MQ=255)

                                                           gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg          <  1:3418881/71‑1 (MQ=255)

                                                            cgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                >  1:3166273/1‑48 (MQ=255)

                                                             gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc        >  1:1557229/1‑71 (MQ=255)

                                                              ccGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc        <  1:2240903/70‑1 (MQ=255)

                                                                gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg     >  1:3315257/1‑71 (MQ=255)

                                                                 aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg          <  1:2927951/65‑1 (MQ=255)

                                                                 aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt    <  1:3936123/71‑1 (MQ=255)

                                                                 aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt    <  1:358038/71‑1 (MQ=255)

                                                                  gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGt         >  1:2357414/1‑65 (MQ=255)

                                                                   cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa  <  1:220481/71‑1 (MQ=255)

TGAGCTTAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGA  >  minE/2570706‑2570843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`