BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,570,779	C→T	6.6%	G228G (GGC→GGT)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,570,779	0	C	T	6.6%	326.6 / 8.1	105	G228G (GGC→GGT)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (48/50); new base T (5/2); total (53/52)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

TAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCT  >  minE/2570712‑2570849

tAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTAc                                                                     >  1:2667099/1‑71 (MQ=255)

 aaGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACg                                                                    >  1:367369/1‑71 (MQ=255)

   gagCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGt                                                                   >  1:2672745/1‑70 (MQ=255)

    agCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                                 >  1:95976/1‑71 (MQ=255)

      cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                               <  1:643878/71‑1 (MQ=255)

      cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                               <  1:2765956/71‑1 (MQ=255)

        cAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                              <  1:1830841/70‑1 (MQ=255)

        cAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                             <  1:2194854/71‑1 (MQ=255)

         aaCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGt                                                                   <  1:783101/64‑1 (MQ=255)

         aaCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                            <  1:50951/71‑1 (MQ=255)

          aCTTACTGAAGCTAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAgg                                                           >  1:3601015/1‑71 (MQ=255)

          aCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                                 >  1:896908/1‑65 (MQ=255)

          aCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                             <  1:3559689/69‑1 (MQ=255)

            ttACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                         >  1:1399077/1‑71 (MQ=255)

             tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                        >  1:1029350/1‑71 (MQ=255)

             tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                        >  1:1561352/1‑71 (MQ=255)

             tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                        >  1:1409704/1‑71 (MQ=255)

              gcggAAGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACt                                                       <  1:2379157/68‑1 (MQ=255)

               cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                       >  1:236619/1‑70 (MQ=255)

               cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                       <  1:2908671/70‑1 (MQ=255)

                tGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                     >  1:2540261/1‑71 (MQ=255)

                tGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                     >  1:46339/1‑71 (MQ=255)

                ggAAGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGa                                                     >  1:2949883/2‑71 (MQ=255)

                 gAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                       <  1:1056164/68‑1 (MQ=255)

                 gAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCCAGCAATGGCt                                                                       <  1:2213941/52‑1 (MQ=255)

                  aaGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                                   >  1:1762874/1‑71 (MQ=255)

                  aaGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGAtt                                                   >  1:2765907/1‑70 (MQ=255)

                   aGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATcc                                                  >  1:1456328/1‑71 (MQ=255)

                   aGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTat                                                                     >  1:1921474/1‑51 (MQ=255)

                    gaaaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                              <  1:1554036/56‑1 (MQ=255)

                    gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCg                                                 >  1:1420436/1‑71 (MQ=255)

                     cAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGc                                                >  1:1267847/1‑71 (MQ=255)

                      aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCt                                                                       <  1:76420/47‑1 (MQ=255)

                      aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                            >  1:3827925/1‑58 (MQ=255)

                      aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                            >  1:2551507/1‑58 (MQ=255)

                      aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                            >  1:203839/1‑58 (MQ=255)

                       aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                             >  1:193785/1‑56 (MQ=255)

                       aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                              >  1:687261/1‑71 (MQ=255)

                       aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                              >  1:2760089/1‑71 (MQ=255)

                            taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                             <  1:2179532/51‑1 (MQ=255)

                            taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                          <  1:228450/70‑1 (MQ=255)

                            taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                         <  1:2530590/71‑1 (MQ=255)

                             atccTTGAGGAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                              <  1:26174/47‑1 (MQ=255)

                             aaCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCt                                                                       <  1:1226409/40‑1 (MQ=255)

                              aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGAt                                                    >  1:1236603/1‑58 (MQ=255)

                              aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                       <  1:172741/71‑1 (MQ=255)

                              aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                       <  1:597022/71‑1 (MQ=255)

                               ccTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                         <  1:3372093/52‑1 (MQ=255)

                                cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCt                                                                       <  1:1013344/37‑1 (MQ=255)

                                cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                      >  1:3429211/1‑70 (MQ=255)

                                cTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTa                                     <  1:605910/71‑1 (MQ=255)

                                   gAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACg                                   <  1:219833/70‑1 (MQ=255)

                                    aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGTTACATACGCa                                 <  1:124614/71‑1 (MQ=255)

                                    aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATAc                                    <  1:2264375/68‑1 (MQ=255)

                                     gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                <  1:1923624/71‑1 (MQ=255)

                                     gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                >  1:3662023/1‑71 (MQ=255)

                                      cAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                <  1:2766676/70‑1 (MQ=255)

                                        gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                        <  1:2252279/44‑1 (MQ=255)

                                        gACTGTAATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                         >  1:1486909/1‑59 (MQ=255)

                                         aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                         <  1:2367045/42‑1 (MQ=255)

                                         aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                            >  1:2567420/1‑55 (MQ=255)

                                          cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGAt                                                    >  1:3114791/1‑46 (MQ=255)

                                             tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                          >  1:3534992/1‑53 (MQ=255)

                                             tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTg                        >  1:2686476/1‑71 (MQ=255)

                                               aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                      >  1:3900848/1‑71 (MQ=255)

                                               aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGc                       <  1:292297/70‑1 (MQ=255)

                                                ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                     >  1:1142127/1‑71 (MQ=255)

                                                ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                     >  1:735532/1‑71 (MQ=255)

                                                ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                     <  1:1976035/71‑1 (MQ=255)

                                                ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                     <  1:2242318/71‑1 (MQ=255)

                                                ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                     <  1:3869112/71‑1 (MQ=255)

                                                ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                     >  1:3757486/1‑71 (MQ=255)

                                                 tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                     >  1:3298004/1‑38 (MQ=255)

                                                 tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                     >  1:3128848/1‑38 (MQ=255)

                                                 tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                     >  1:523345/1‑38 (MQ=255)

                                                 tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                     >  1:124238/1‑38 (MQ=255)

                                                  cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                   >  1:3179459/1‑71 (MQ=255)

                                                  cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                   >  1:3438704/1‑71 (MQ=255)

                                                   gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                   <  1:481804/70‑1 (MQ=255)

                                                   gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                   <  1:664843/70‑1 (MQ=255)

                                                     gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                <  1:43089/71‑1 (MQ=255)

                                                      cgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                      >  1:3166273/1‑48 (MQ=255)

                                                       gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc              >  1:1557229/1‑71 (MQ=255)

                                                        ccGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc              <  1:2240903/70‑1 (MQ=255)

                                                          gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg           >  1:3315257/1‑71 (MQ=255)

                                                           aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                <  1:2927951/65‑1 (MQ=255)

                                                           aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt          <  1:358038/71‑1 (MQ=255)

                                                           aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt          <  1:3936123/71‑1 (MQ=255)

                                                            gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGt               >  1:2357414/1‑65 (MQ=255)

                                                             cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa        <  1:220481/71‑1 (MQ=255)

                                                             cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa        <  1:1659244/71‑1 (MQ=255)

                                                               aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg      <  1:2915519/71‑1 (MQ=255)

                                                               aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg      >  1:895186/1‑71 (MQ=255)

                                                                tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                   <  1:3269496/57‑1 (MQ=255)

                                                                tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGAt       <  1:1699239/69‑1 (MQ=255)

                                                                 ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg     >  1:418068/1‑70 (MQ=255)

                                                                 ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt    >  1:3459218/1‑71 (MQ=255)

                                                                  gCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                       <  1:944794/35‑1 (MQ=255)

                                                                  gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc   >  1:3838290/1‑71 (MQ=255)

                                                                   cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt  <  1:2534870/71‑1 (MQ=255)

TAAGAGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCT  >  minE/2570712‑2570849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`