BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,570,785	T→G	6.0%	V230V (GTT→GTG)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,570,785	0	T	G	6.0%	264.0 / 10.1	100	V230V (GTT→GTG)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (47/47); new base G (4/2); total (51/49)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 101 total aligned reads displayed.

AGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATC  >  minE/2570716‑2570854

agCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                                      >  1:95976/1‑71 (MQ=255)

  cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                                    <  1:2765956/71‑1 (MQ=255)

  cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                                    <  1:643878/71‑1 (MQ=255)

   gcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                                   <  1:457105/71‑1 (MQ=255)

    cAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                                   <  1:1830841/70‑1 (MQ=255)

    cAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                                  <  1:2194854/71‑1 (MQ=255)

     aaCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                                 <  1:50951/71‑1 (MQ=255)

      aCTTACTGAAGCTAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAgg                                                                >  1:3601015/1‑71 (MQ=255)

      aCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                                      >  1:896908/1‑65 (MQ=255)

      aCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                                  <  1:3559689/69‑1 (MQ=255)

        ttACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                              >  1:1399077/1‑71 (MQ=255)

         tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                             >  1:1029350/1‑71 (MQ=255)

         tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                             >  1:1561352/1‑71 (MQ=255)

         tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                             >  1:1409704/1‑71 (MQ=255)

          gcggAAGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACt                                                            <  1:2379157/68‑1 (MQ=255)

           cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                            <  1:1591148/70‑1 (MQ=255)

           cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                            <  1:2908671/70‑1 (MQ=255)

           cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                            >  1:236619/1‑70 (MQ=255)

            tGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                          >  1:2540261/1‑71 (MQ=255)

            tGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                          >  1:46339/1‑71 (MQ=255)

            ggAAGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGa                                                          >  1:2949883/2‑71 (MQ=255)

             gAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                            <  1:1056164/68‑1 (MQ=255)

              aaGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGAt                                                         >  1:2247349/1‑70 (MQ=255)

              aaGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                                        >  1:1762874/1‑71 (MQ=255)

              aaGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGAtt                                                        >  1:2765907/1‑70 (MQ=255)

               aGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATcc                                                       >  1:1456328/1‑71 (MQ=255)

                gaaaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                                   <  1:1554036/56‑1 (MQ=255)

                gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCg                                                      >  1:1420436/1‑71 (MQ=255)

                 cAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGc                                                     >  1:1267847/1‑71 (MQ=255)

                  aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                                 >  1:2551507/1‑58 (MQ=255)

                  aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                                 >  1:3827925/1‑58 (MQ=255)

                  aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                                 >  1:203839/1‑58 (MQ=255)

                   aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                                  >  1:193785/1‑56 (MQ=255)

                   aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                                   >  1:687261/1‑71 (MQ=255)

                   aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                                   >  1:2760089/1‑71 (MQ=255)

                        taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                                  <  1:2179532/51‑1 (MQ=255)

                        taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                               <  1:228450/70‑1 (MQ=255)

                        taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                              <  1:2530590/71‑1 (MQ=255)

                         atccTTGAGGAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                                   <  1:26174/47‑1 (MQ=255)

                          aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGAt                                                         >  1:1236603/1‑58 (MQ=255)

                          aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                            <  1:172741/71‑1 (MQ=255)

                          aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                            <  1:597022/71‑1 (MQ=255)

                           ccTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                              <  1:3372093/52‑1 (MQ=255)

                            cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                           >  1:3429211/1‑70 (MQ=255)

                            cTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTa                                          <  1:605910/71‑1 (MQ=255)

                               gAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACg                                        <  1:219833/70‑1 (MQ=255)

                                aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGTTACATACGCa                                      <  1:124614/71‑1 (MQ=255)

                                aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATAc                                         <  1:2264375/68‑1 (MQ=255)

                                 gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                     <  1:1923624/71‑1 (MQ=255)

                                 gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                     >  1:3662023/1‑71 (MQ=255)

                                  cAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                     <  1:2766676/70‑1 (MQ=255)

                                    gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                             <  1:2252279/44‑1 (MQ=255)

                                    gACTGTAATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                              >  1:1486909/1‑59 (MQ=255)

                                     aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                              <  1:2367045/42‑1 (MQ=255)

                                     aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                                 >  1:2567420/1‑55 (MQ=255)

                                      cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGAt                                                         >  1:3114791/1‑46 (MQ=255)

                                      cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGcc                                <  1:2710821/71‑1 (MQ=255)

                                         tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                               >  1:3534992/1‑53 (MQ=255)

                                         tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTg                             >  1:2686476/1‑71 (MQ=255)

                                           aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                           >  1:3900848/1‑71 (MQ=255)

                                           aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGc                            <  1:292297/70‑1 (MQ=255)

                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          >  1:735532/1‑71 (MQ=255)

                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          >  1:1142127/1‑71 (MQ=255)

                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          <  1:1976035/71‑1 (MQ=255)

                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          <  1:3869112/71‑1 (MQ=255)

                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          >  1:3757486/1‑71 (MQ=255)

                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          <  1:2242318/71‑1 (MQ=255)

                                             tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                          >  1:523345/1‑38 (MQ=255)

                                             tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                          >  1:124238/1‑38 (MQ=255)

                                             tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                          >  1:3128848/1‑38 (MQ=255)

                                             tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                          >  1:3298004/1‑38 (MQ=255)

                                              cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                        >  1:3438704/1‑71 (MQ=255)

                                              cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                        >  1:3179459/1‑71 (MQ=255)

                                               gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                        <  1:481804/70‑1 (MQ=255)

                                               gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                        <  1:664843/70‑1 (MQ=255)

                                                 gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                     <  1:3418881/71‑1 (MQ=255)

                                                 gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                     <  1:43089/71‑1 (MQ=255)

                                                  cgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                           >  1:3166273/1‑48 (MQ=255)

                                                   gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                   >  1:1557229/1‑71 (MQ=255)

                                                    ccGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                   <  1:2240903/70‑1 (MQ=255)

                                                      gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                >  1:3315257/1‑71 (MQ=255)

                                                       aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                     <  1:2927951/65‑1 (MQ=255)

                                                       aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt               <  1:358038/71‑1 (MQ=255)

                                                       aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt               <  1:3936123/71‑1 (MQ=255)

                                                        gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGt                    >  1:2357414/1‑65 (MQ=255)

                                                         cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa             <  1:1659244/71‑1 (MQ=255)

                                                         cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa             <  1:220481/71‑1 (MQ=255)

                                                           aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg           >  1:895186/1‑71 (MQ=255)

                                                           aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg           <  1:2915519/71‑1 (MQ=255)

                                                            tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                        <  1:3269496/57‑1 (MQ=255)

                                                            tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGAt            <  1:1699239/69‑1 (MQ=255)

                                                             ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg          >  1:418068/1‑70 (MQ=255)

                                                             ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt         >  1:3459218/1‑71 (MQ=255)

                                                              gCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                            <  1:944794/35‑1 (MQ=255)

                                                              gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc        >  1:3838290/1‑71 (MQ=255)

                                                               cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt       <  1:2534870/71‑1 (MQ=255)

                                                                  cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa    >  1:3808903/1‑71 (MQ=255)

                                                                   gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                           >  1:1883697/1‑47 (MQ=255)

                                                                   gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc     <  1:632607/69‑1 (MQ=255)

                                                                     tACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc  >  1:2892101/1‑70 (MQ=255)

AGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATC  >  minE/2570716‑2570854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`