BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,570,788	T→C	5.9%	T231T (ACT→ACC)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,570,788	0	T	C	5.9%	296.9 / 7.0	102	T231T (ACT→ACC)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (47/49); new base C (4/2); total (51/51)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCC  >  minE/2570718‑2570857

cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                                       <  1:643878/71‑1 (MQ=255)

cgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                                       <  1:2765956/71‑1 (MQ=255)

 gcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                                      <  1:457105/71‑1 (MQ=255)

  cAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                                      <  1:1830841/70‑1 (MQ=255)

  cAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                                     <  1:2194854/71‑1 (MQ=255)

   aaCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                                    <  1:50951/71‑1 (MQ=255)

    aCTTACTGAAGCTAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAgg                                                                   >  1:3601015/1‑71 (MQ=255)

    aCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                                     <  1:3559689/69‑1 (MQ=255)

      ttACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                                 >  1:1399077/1‑71 (MQ=255)

       tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                                >  1:1409704/1‑71 (MQ=255)

       tACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                                >  1:1029350/1‑71 (MQ=255)

        gcggAAGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACt                                                               <  1:2379157/68‑1 (MQ=255)

         cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                               <  1:2908671/70‑1 (MQ=255)

         cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                               <  1:1591148/70‑1 (MQ=255)

         cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                               >  1:236619/1‑70 (MQ=255)

          tGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                             >  1:2540261/1‑71 (MQ=255)

          tGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                             >  1:46339/1‑71 (MQ=255)

          ggAAGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGa                                                             >  1:2949883/2‑71 (MQ=255)

           gAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                               <  1:1056164/68‑1 (MQ=255)

            aaGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGAt                                                            >  1:2247349/1‑70 (MQ=255)

            aaGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                                           >  1:1762874/1‑71 (MQ=255)

            aaGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGAtt                                                           >  1:2765907/1‑70 (MQ=255)

             aGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATcc                                                          >  1:1456328/1‑71 (MQ=255)

              gaaaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                                      <  1:1554036/56‑1 (MQ=255)

              gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCg                                                         >  1:1420436/1‑71 (MQ=255)

               cAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGc                                                        >  1:1267847/1‑71 (MQ=255)

                aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                                    >  1:3827925/1‑58 (MQ=255)

                aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                                    >  1:203839/1‑58 (MQ=255)

                aaaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAg                                                                    >  1:2551507/1‑58 (MQ=255)

                 aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                                     >  1:193785/1‑56 (MQ=255)

                 aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                                      >  1:687261/1‑71 (MQ=255)

                 aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                                      >  1:2760089/1‑71 (MQ=255)

                      taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCa                                                                     <  1:2179532/51‑1 (MQ=255)

                      taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                                  <  1:228450/70‑1 (MQ=255)

                      taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                                 <  1:2530590/71‑1 (MQ=255)

                       atccTTGAGGAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTc                                                                      <  1:26174/47‑1 (MQ=255)

                        aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGAt                                                            >  1:1236603/1‑58 (MQ=255)

                        aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                               <  1:172741/71‑1 (MQ=255)

                        aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                               <  1:597022/71‑1 (MQ=255)

                         ccTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                                 <  1:3372093/52‑1 (MQ=255)

                          cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                              >  1:3429211/1‑70 (MQ=255)

                          cTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTa                                             <  1:605910/71‑1 (MQ=255)

                             gAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACg                                           <  1:219833/70‑1 (MQ=255)

                              aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGTTACATACGCa                                         <  1:124614/71‑1 (MQ=255)

                              aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATAc                                            <  1:2264375/68‑1 (MQ=255)

                               gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                        >  1:3662023/1‑71 (MQ=255)

                               gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                        <  1:1923624/71‑1 (MQ=255)

                                cAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                        <  1:2766676/70‑1 (MQ=255)

                                  gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTAc                                                                <  1:2252279/44‑1 (MQ=255)

                                  gACTGTAATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                                 >  1:1486909/1‑59 (MQ=255)

                                   aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                                 <  1:2367045/42‑1 (MQ=255)

                                   aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                                    >  1:2567420/1‑55 (MQ=255)

                                    cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGAt                                                            >  1:3114791/1‑46 (MQ=255)

                                    cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGcc                                   <  1:2710821/71‑1 (MQ=255)

                                       tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                                  >  1:3534992/1‑53 (MQ=255)

                                       tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTg                                >  1:2686476/1‑71 (MQ=255)

                                         aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                              >  1:3900848/1‑71 (MQ=255)

                                         aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGc                               <  1:292297/70‑1 (MQ=255)

                                          ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                             <  1:1976035/71‑1 (MQ=255)

                                          ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                             >  1:735532/1‑71 (MQ=255)

                                          ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                             >  1:1142127/1‑71 (MQ=255)

                                          ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                             <  1:2242318/71‑1 (MQ=255)

                                          ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                             >  1:3757486/1‑71 (MQ=255)

                                          ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                             <  1:3869112/71‑1 (MQ=255)

                                           tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                             >  1:523345/1‑38 (MQ=255)

                                           tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                             >  1:124238/1‑38 (MQ=255)

                                           tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                             >  1:3298004/1‑38 (MQ=255)

                                           tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                             >  1:3128848/1‑38 (MQ=255)

                                            cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                           >  1:3179459/1‑71 (MQ=255)

                                            cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                           >  1:3438704/1‑71 (MQ=255)

                                             gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                           <  1:481804/70‑1 (MQ=255)

                                             gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                           <  1:664843/70‑1 (MQ=255)

                                               gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                        <  1:3418881/71‑1 (MQ=255)

                                                cgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                              >  1:3166273/1‑48 (MQ=255)

                                                 gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                      >  1:1557229/1‑71 (MQ=255)

                                                  ccGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                      <  1:2240903/70‑1 (MQ=255)

                                                    gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                   >  1:3315257/1‑71 (MQ=255)

                                                     aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt                  <  1:358038/71‑1 (MQ=255)

                                                     aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt                  <  1:3936123/71‑1 (MQ=255)

                                                      gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGt                       >  1:2357414/1‑65 (MQ=255)

                                                       cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                <  1:1659244/71‑1 (MQ=255)

                                                         aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg              >  1:895186/1‑71 (MQ=255)

                                                         aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg              <  1:2915519/71‑1 (MQ=255)

                                                          tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                           <  1:3269496/57‑1 (MQ=255)

                                                          tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGAt               <  1:1699239/69‑1 (MQ=255)

                                                           ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg             >  1:418068/1‑70 (MQ=255)

                                                            gCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                               <  1:944794/35‑1 (MQ=255)

                                                            gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc           >  1:3838290/1‑71 (MQ=255)

                                                                cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa       >  1:3808903/1‑71 (MQ=255)

                                                                 gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                              >  1:1883697/1‑47 (MQ=255)

                                                                 gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc        <  1:632607/69‑1 (MQ=255)

                                                                   tACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc     >  1:2892101/1‑70 (MQ=255)

                                                                    aCTCAGGTACTGATCCGACCAGGTACATACGCAGCTGc                                    >  1:2568680/1‑38 (MQ=255)

                                                                     cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcccc  <  1:2316874/71‑1 (MQ=255)

                                                                     cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc   <  1:1413914/70‑1 (MQ=255)

                                                                     cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc   <  1:315582/70‑1 (MQ=255)

                                                                     cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc   <  1:3493113/70‑1 (MQ=255)

                                                                      tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGTTGCGTCCGGTGAt               <  1:2296213/57‑1 (MQ=255)

                                                                      tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGAt               <  1:2331357/57‑1 (MQ=255)

                                                                      tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt            <  1:2933217/60‑1 (MQ=255)

CGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCC  >  minE/2570718‑2570857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`