Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,570,800	C→T	8.7%	I235I (ATC→ATT)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,570,800	0	C	T	8.7%	234.4 / 9.3	80	I235I (ATC→ATT)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/44);  new base T (5/2);  total (34/46)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGG  >  minE/2570730‑2570869                                                                       |                                                                      aaGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                                                       >  1:1762874/1‑71 (MQ=255)  aGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATcc                                                                      >  1:1456328/1‑71 (MQ=255)   gCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCg                                                                     >  1:1420436/1‑71 (MQ=255)    cAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGc                                                                    >  1:1267847/1‑71 (MQ=255)      aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                                                  >  1:2760089/1‑71 (MQ=255)      aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                                                  >  1:687261/1‑71 (MQ=255)           taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                                              <  1:228450/70‑1 (MQ=255)           taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                                             <  1:2530590/71‑1 (MQ=255)             aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                           <  1:597022/71‑1 (MQ=255)             aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                           <  1:172741/71‑1 (MQ=255)               cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                                          >  1:3429211/1‑70 (MQ=255)               cTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTa                                                         <  1:605910/71‑1 (MQ=255)                  gAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACg                                                       <  1:219833/70‑1 (MQ=255)                   aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGTTACATACGCa                                                     <  1:124614/71‑1 (MQ=255)                   aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATAc                                                        <  1:2264375/68‑1 (MQ=255)                    gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                                    <  1:1923624/71‑1 (MQ=255)                    gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                                    >  1:3662023/1‑71 (MQ=255)                     cAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                                    <  1:2766676/70‑1 (MQ=255)                       gACTGTAATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                                             >  1:1486909/1‑59 (MQ=255)                        aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                                                >  1:2567420/1‑55 (MQ=255)                         cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGcc                                               <  1:2710821/71‑1 (MQ=255)                            tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                                              >  1:3534992/1‑53 (MQ=255)                            tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTg                                            >  1:2686476/1‑71 (MQ=255)                              aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                          >  1:3900848/1‑71 (MQ=255)                              aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGc                                           <  1:292297/70‑1 (MQ=255)                               ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                         <  1:2242318/71‑1 (MQ=255)                               ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                         >  1:735532/1‑71 (MQ=255)                               ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                         >  1:1142127/1‑71 (MQ=255)                               ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                         <  1:1976035/71‑1 (MQ=255)                               ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                         <  1:3869112/71‑1 (MQ=255)                               ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                         >  1:3757486/1‑71 (MQ=255)                                 cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                       >  1:3179459/1‑71 (MQ=255)                                 cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                       >  1:3438704/1‑71 (MQ=255)                                  gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                       <  1:481804/70‑1 (MQ=255)                                  gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                       <  1:664843/70‑1 (MQ=255)                                    gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                                    <  1:3418881/71‑1 (MQ=255)                                    gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                                    <  1:43089/71‑1 (MQ=255)                                     cgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                                          >  1:3166273/1‑48 (MQ=255)                                      gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                  >  1:1557229/1‑71 (MQ=255)                                       ccGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                  <  1:2240903/70‑1 (MQ=255)                                         gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                               >  1:3315257/1‑71 (MQ=255)                                          aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                                    <  1:2927951/65‑1 (MQ=255)                                          aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt                              <  1:358038/71‑1 (MQ=255)                                          aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt                              <  1:3936123/71‑1 (MQ=255)                                           gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGt                                   >  1:2357414/1‑65 (MQ=255)                                            cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                            <  1:220481/71‑1 (MQ=255)                                            cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                            <  1:1659244/71‑1 (MQ=255)                                              aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg                          <  1:2915519/71‑1 (MQ=255)                                              aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg                          >  1:895186/1‑71 (MQ=255)                                               tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                       <  1:3269496/57‑1 (MQ=255)                                               tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGAt                           <  1:1699239/69‑1 (MQ=255)                                                ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg                         >  1:418068/1‑70 (MQ=255)                                                ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                        >  1:3459218/1‑71 (MQ=255)                                                 gCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                           <  1:944794/35‑1 (MQ=255)                                                 gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                       >  1:3838290/1‑71 (MQ=255)                                                  cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                      <  1:2534870/71‑1 (MQ=255)                                                     cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                   >  1:3808903/1‑71 (MQ=255)                                                      gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                          >  1:1883697/1‑47 (MQ=255)                                                      gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                    <  1:632607/69‑1 (MQ=255)                                                        tACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc                 >  1:2892101/1‑70 (MQ=255)                                                         aCTCAGGTACTGATCCGACCAGGTACATACGCAGCTGc                                                >  1:2568680/1‑38 (MQ=255)                                                          cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcccc              <  1:2316874/71‑1 (MQ=255)                                                          cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc               <  1:315582/70‑1 (MQ=255)                                                          cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc               <  1:3493113/70‑1 (MQ=255)                                                          cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc               <  1:1413914/70‑1 (MQ=255)                                                           tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGTTGCGTCCGGTGAt                           <  1:2296213/57‑1 (MQ=255)                                                           tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGAt                           <  1:2331357/57‑1 (MQ=255)                                                           tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                        <  1:2933217/60‑1 (MQ=255)                                                            cAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGa            >  1:3094513/1‑71 (MQ=255)                                                             aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGat           >  1:1626206/1‑70 (MQ=255)                                                             aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGat           >  1:1277992/1‑70 (MQ=255)                                                             aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg           >  1:3257161/1‑71 (MQ=255)                                                               gTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGtgt                      <  1:289122/58‑3 (MQ=255)                                                               gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                       <  1:2413744/41‑1 (MQ=255)                                                               gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                    >  1:2573784/1‑60 (MQ=255)                                                               gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCa         <  1:2080786/71‑1 (MQ=255)                                                                tACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaa        <  1:3020262/71‑1 (MQ=255)                                                                 aCTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGc                                     >  1:2165715/1‑41 (MQ=255)                                                                  cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa      <  1:2419635/71‑1 (MQ=255)                                                                  cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa       <  1:70292/70‑1 (MQ=255)                                                                     aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACg   <  1:660131/71‑1 (MQ=255)                                                                      tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCGAAAACgg  <  1:3952017/71‑1 (MQ=255)                                                                       |                                                                      AAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGG  >  minE/2570730‑2570869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑