Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,570,803 C→T 6.6% R236R (CGC→CGT)  yibH → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,570,8030CT 6.6% 231.9 / 4.6 76R236R (CGC→CGT) yibHhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (26/44);  new base T (3/2);  total (30/46)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

CAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAA  >  minE/2570733‑2570873
                                                                      |                                                                      
cAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGc                                                                        >  1:1267847/1‑71 (MQ=255)
  aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                                                      >  1:2760089/1‑71 (MQ=255)
  aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                                                      >  1:687261/1‑71 (MQ=255)
       taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                                                  <  1:228450/70‑1 (MQ=255)
       taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                                                 <  1:2530590/71‑1 (MQ=255)
         aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                               <  1:597022/71‑1 (MQ=255)
         aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                               <  1:172741/71‑1 (MQ=255)
           cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                                              >  1:3429211/1‑70 (MQ=255)
           cTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTa                                                             <  1:605910/71‑1 (MQ=255)
              gAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACg                                                           <  1:219833/70‑1 (MQ=255)
               aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGTTACATACGCa                                                         <  1:124614/71‑1 (MQ=255)
               aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATAc                                                            <  1:2264375/68‑1 (MQ=255)
                gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                                        <  1:1923624/71‑1 (MQ=255)
                gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                                        >  1:3662023/1‑71 (MQ=255)
                 cAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                                        <  1:2766676/70‑1 (MQ=255)
                   gACTGTAATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                                                 >  1:1486909/1‑59 (MQ=255)
                    aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                                                    >  1:2567420/1‑55 (MQ=255)
                     cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGcc                                                   <  1:2710821/71‑1 (MQ=255)
                        tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                                                  >  1:3534992/1‑53 (MQ=255)
                        tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTg                                                >  1:2686476/1‑71 (MQ=255)
                          aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                              >  1:3900848/1‑71 (MQ=255)
                          aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGc                                               <  1:292297/70‑1 (MQ=255)
                           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                             <  1:2242318/71‑1 (MQ=255)
                           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                             >  1:1142127/1‑71 (MQ=255)
                           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                             <  1:3869112/71‑1 (MQ=255)
                           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                             >  1:3757486/1‑71 (MQ=255)
                           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                             >  1:735532/1‑71 (MQ=255)
                           ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                             <  1:1976035/71‑1 (MQ=255)
                             cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                           >  1:3179459/1‑71 (MQ=255)
                             cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                           >  1:3438704/1‑71 (MQ=255)
                              gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                           <  1:481804/70‑1 (MQ=255)
                              gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                           <  1:664843/70‑1 (MQ=255)
                                gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                                        <  1:3418881/71‑1 (MQ=255)
                                gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                                        <  1:43089/71‑1 (MQ=255)
                                 cgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                                              >  1:3166273/1‑48 (MQ=255)
                                  gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                      >  1:1557229/1‑71 (MQ=255)
                                   ccGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                      <  1:2240903/70‑1 (MQ=255)
                                     gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                   >  1:3315257/1‑71 (MQ=255)
                                      aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                                        <  1:2927951/65‑1 (MQ=255)
                                      aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt                                  <  1:358038/71‑1 (MQ=255)
                                      aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt                                  <  1:3936123/71‑1 (MQ=255)
                                       gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGt                                       >  1:2357414/1‑65 (MQ=255)
                                        cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                                <  1:220481/71‑1 (MQ=255)
                                        cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                                <  1:1659244/71‑1 (MQ=255)
                                          aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg                              >  1:895186/1‑71 (MQ=255)
                                          aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg                              <  1:2915519/71‑1 (MQ=255)
                                           tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                           <  1:3269496/57‑1 (MQ=255)
                                           tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGAt                               <  1:1699239/69‑1 (MQ=255)
                                            ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg                             >  1:418068/1‑70 (MQ=255)
                                            ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                            >  1:3459218/1‑71 (MQ=255)
                                             gCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                               <  1:944794/35‑1 (MQ=255)
                                             gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                           >  1:3838290/1‑71 (MQ=255)
                                              cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                          <  1:2534870/71‑1 (MQ=255)
                                                 cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                       >  1:3808903/1‑71 (MQ=255)
                                                  gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                              >  1:1883697/1‑47 (MQ=255)
                                                  gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                        <  1:632607/69‑1 (MQ=255)
                                                    tACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc                     >  1:2892101/1‑70 (MQ=255)
                                                     aCTCAGGTACTGATCCGACCAGGTACATACGCAGCTGc                                                    >  1:2568680/1‑38 (MQ=255)
                                                      cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcccc                  <  1:2316874/71‑1 (MQ=255)
                                                      cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                   <  1:315582/70‑1 (MQ=255)
                                                      cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                   <  1:1413914/70‑1 (MQ=255)
                                                      cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                   <  1:3493113/70‑1 (MQ=255)
                                                       tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGTTGCGTCCGGTGAt                               <  1:2296213/57‑1 (MQ=255)
                                                       tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGAt                               <  1:2331357/57‑1 (MQ=255)
                                                       tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                            <  1:2933217/60‑1 (MQ=255)
                                                        cAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGa                >  1:3094513/1‑71 (MQ=255)
                                                         aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGat               >  1:1277992/1‑70 (MQ=255)
                                                         aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGat               >  1:1626206/1‑70 (MQ=255)
                                                         aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg               >  1:3257161/1‑71 (MQ=255)
                                                           gTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGtgt                          <  1:289122/58‑3 (MQ=255)
                                                           gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                           <  1:2413744/41‑1 (MQ=255)
                                                           gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                        >  1:2573784/1‑60 (MQ=255)
                                                           gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCa             <  1:2080786/71‑1 (MQ=255)
                                                            tACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaa            <  1:3020262/71‑1 (MQ=255)
                                                             aCTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGc                                         >  1:2165715/1‑41 (MQ=255)
                                                              cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa          <  1:2419635/71‑1 (MQ=255)
                                                              cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa           <  1:70292/70‑1 (MQ=255)
                                                                 aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACg       <  1:660131/71‑1 (MQ=255)
                                                                  tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCGAAAACgg      <  1:3952017/71‑1 (MQ=255)
                                                                      cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                   <  1:1866897/54‑1 (MQ=255)
                                                                      cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCaaa  <  1:3843591/71‑1 (MQ=255)
                                                                      cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCaa   <  1:2235528/70‑1 (MQ=255)
                                                                      |                                                                      
CAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAA  >  minE/2570733‑2570873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑