Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,570,806	A→G	6.2%	P237P (CCA→CCG)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,570,806	0	A	G	6.2%	231.4 / 4.9	80	P237P (CCA→CCG)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/47);  new base G (3/2);  total (31/49)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

TAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTG  >  minE/2570740‑2570876                                                                   |                                                                       taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                                                     <  1:228450/70‑1 (MQ=255) taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                                                    <  1:2530590/71‑1 (MQ=255)   aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                                  <  1:597022/71‑1 (MQ=255)   aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                                  <  1:172741/71‑1 (MQ=255)     cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                                                 >  1:3429211/1‑70 (MQ=255)     cTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTa                                                                <  1:605910/71‑1 (MQ=255)        gAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACg                                                              <  1:219833/70‑1 (MQ=255)         aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGTTACATACGCa                                                            <  1:124614/71‑1 (MQ=255)         aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATAc                                                               <  1:2264375/68‑1 (MQ=255)          gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                                           <  1:1923624/71‑1 (MQ=255)          gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                                           >  1:3662023/1‑71 (MQ=255)           cAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                                           <  1:2766676/70‑1 (MQ=255)             gACTGTAATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                                                    >  1:1486909/1‑59 (MQ=255)              aCTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                                                       >  1:2567420/1‑55 (MQ=255)               cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGcc                                                      <  1:2710821/71‑1 (MQ=255)                  tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGt                                                                     >  1:3534992/1‑53 (MQ=255)                  tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTg                                                   >  1:2686476/1‑71 (MQ=255)                    aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                 >  1:3900848/1‑71 (MQ=255)                    aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGc                                                  <  1:292297/70‑1 (MQ=255)                     ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                >  1:1142127/1‑71 (MQ=255)                     ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                >  1:3757486/1‑71 (MQ=255)                     ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                <  1:2242318/71‑1 (MQ=255)                     ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                >  1:735532/1‑71 (MQ=255)                     ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                <  1:3869112/71‑1 (MQ=255)                     ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                <  1:1976035/71‑1 (MQ=255)                       cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                              >  1:3179459/1‑71 (MQ=255)                       cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                              >  1:3438704/1‑71 (MQ=255)                        gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                              <  1:664843/70‑1 (MQ=255)                        gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                              <  1:481804/70‑1 (MQ=255)                          gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                                           <  1:43089/71‑1 (MQ=255)                          gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                                           <  1:3418881/71‑1 (MQ=255)                           cgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                                                 >  1:3166273/1‑48 (MQ=255)                            gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                         >  1:1557229/1‑71 (MQ=255)                             ccGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                         <  1:2240903/70‑1 (MQ=255)                               gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                      >  1:3315257/1‑71 (MQ=255)                                aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                                           <  1:2927951/65‑1 (MQ=255)                                aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt                                     <  1:3936123/71‑1 (MQ=255)                                aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt                                     <  1:358038/71‑1 (MQ=255)                                 gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGt                                          >  1:2357414/1‑65 (MQ=255)                                  cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                                   <  1:1659244/71‑1 (MQ=255)                                  cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                                   <  1:220481/71‑1 (MQ=255)                                    aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg                                 <  1:2915519/71‑1 (MQ=255)                                    aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg                                 >  1:895186/1‑71 (MQ=255)                                     tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                              <  1:3269496/57‑1 (MQ=255)                                     tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGAt                                  <  1:1699239/69‑1 (MQ=255)                                      ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg                                >  1:418068/1‑70 (MQ=255)                                      ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                               >  1:3459218/1‑71 (MQ=255)                                       gCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                                  <  1:944794/35‑1 (MQ=255)                                       gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                              >  1:3838290/1‑71 (MQ=255)                                        cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                             <  1:2534870/71‑1 (MQ=255)                                           cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                          >  1:3808903/1‑71 (MQ=255)                                            gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                 >  1:1883697/1‑47 (MQ=255)                                            gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                           <  1:632607/69‑1 (MQ=255)                                              tACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc                        >  1:2892101/1‑70 (MQ=255)                                               aCTCAGGTACTGATCCGACCAGGTACATACGCAGCTGc                                                       >  1:2568680/1‑38 (MQ=255)                                                cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcccc                     <  1:2316874/71‑1 (MQ=255)                                                cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                      <  1:1413914/70‑1 (MQ=255)                                                cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                      <  1:3493113/70‑1 (MQ=255)                                                cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                      <  1:315582/70‑1 (MQ=255)                                                 tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGTTGCGTCCGGTGAt                                  <  1:2296213/57‑1 (MQ=255)                                                 tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGAt                                  <  1:2331357/57‑1 (MQ=255)                                                 tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                               <  1:2933217/60‑1 (MQ=255)                                                  cAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGa                   >  1:3094513/1‑71 (MQ=255)                                                   aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGat                  >  1:1277992/1‑70 (MQ=255)                                                   aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGat                  >  1:1626206/1‑70 (MQ=255)                                                   aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                  >  1:3257161/1‑71 (MQ=255)                                                     gTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGtgt                             <  1:289122/58‑3 (MQ=255)                                                     gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                              <  1:2413744/41‑1 (MQ=255)                                                     gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                           >  1:2573784/1‑60 (MQ=255)                                                     gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCa                <  1:2080786/71‑1 (MQ=255)                                                      tACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaa               <  1:3020262/71‑1 (MQ=255)                                                       aCTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGc                                            >  1:2165715/1‑41 (MQ=255)                                                        cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa             <  1:2419635/71‑1 (MQ=255)                                                        cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa              <  1:70292/70‑1 (MQ=255)                                                           aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACg          <  1:660131/71‑1 (MQ=255)                                                            tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCGAAAACgg         <  1:3952017/71‑1 (MQ=255)                                                                cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                      <  1:1866897/54‑1 (MQ=255)                                                                cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCaaa     <  1:3843591/71‑1 (MQ=255)                                                                cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCaa      <  1:2235528/70‑1 (MQ=255)                                                                 ccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAAt    >  1:2956086/1‑71 (MQ=255)                                                                   aGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTATTCATCCCCGAGCAAAAACGGCAAATTg  <  1:1975554/71‑1 (MQ=255)                                                                   |                                                                       TAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTG  >  minE/2570740‑2570876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑