Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,570,812 A→C 6.7% T239T (ACA→ACC)  yibH → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,570,8120AC 6.7% 194.2 / 7.8 75T239T (ACA→ACC) yibHhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (27/43);  new base C (3/2);  total (30/45)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

ACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGC  >  minE/2570742‑2570880
                                                                      |                                                                    
aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                                      <  1:597022/71‑1 (MQ=255)
aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                                      <  1:172741/71‑1 (MQ=255)
  cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                                                     >  1:3429211/1‑70 (MQ=255)
  cTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTa                                                                    <  1:605910/71‑1 (MQ=255)
     gAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACg                                                                  <  1:219833/70‑1 (MQ=255)
      aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGTTACATACGCa                                                                <  1:124614/71‑1 (MQ=255)
      aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATAc                                                                   <  1:2264375/68‑1 (MQ=255)
       gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                                               >  1:3662023/1‑71 (MQ=255)
       gCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                                               <  1:1923624/71‑1 (MQ=255)
        cAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAg                                                               <  1:2766676/70‑1 (MQ=255)
            cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGcc                                                          <  1:2710821/71‑1 (MQ=255)
               tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTg                                                       >  1:2686476/1‑71 (MQ=255)
                 aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                     >  1:3900848/1‑71 (MQ=255)
                 aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGc                                                      <  1:292297/70‑1 (MQ=255)
                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    >  1:735532/1‑71 (MQ=255)
                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    <  1:1976035/71‑1 (MQ=255)
                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    >  1:1142127/1‑71 (MQ=255)
                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    <  1:2242318/71‑1 (MQ=255)
                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    >  1:3757486/1‑71 (MQ=255)
                  ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                    <  1:3869112/71‑1 (MQ=255)
                    cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                                  >  1:3179459/1‑71 (MQ=255)
                    cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                                  >  1:3438704/1‑71 (MQ=255)
                     gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                                  <  1:664843/70‑1 (MQ=255)
                     gcgcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                                  <  1:481804/70‑1 (MQ=255)
                       gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                                               <  1:3418881/71‑1 (MQ=255)
                       gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                                               <  1:43089/71‑1 (MQ=255)
                        cgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACAt                                                                     >  1:3166273/1‑48 (MQ=255)
                         gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                             >  1:1557229/1‑71 (MQ=255)
                          ccGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                             <  1:2240903/70‑1 (MQ=255)
                            gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                          >  1:3315257/1‑71 (MQ=255)
                             aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCg                                               <  1:2927951/65‑1 (MQ=255)
                             aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt                                         <  1:3936123/71‑1 (MQ=255)
                             aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGt                                         <  1:358038/71‑1 (MQ=255)
                              gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGt                                              >  1:2357414/1‑65 (MQ=255)
                               cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                                       <  1:1659244/71‑1 (MQ=255)
                               cAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                                       <  1:220481/71‑1 (MQ=255)
                                 aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg                                     <  1:2915519/71‑1 (MQ=255)
                                 aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATg                                     >  1:895186/1‑71 (MQ=255)
                                  tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                                  <  1:3269496/57‑1 (MQ=255)
                                  tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGAt                                      <  1:1699239/69‑1 (MQ=255)
                                   ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg                                    >  1:418068/1‑70 (MQ=255)
                                   ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                                   >  1:3459218/1‑71 (MQ=255)
                                    gCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                                                      <  1:944794/35‑1 (MQ=255)
                                    gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                  >  1:3838290/1‑71 (MQ=255)
                                     cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                                 <  1:2534870/71‑1 (MQ=255)
                                        cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                              >  1:3808903/1‑71 (MQ=255)
                                         gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                     >  1:1883697/1‑47 (MQ=255)
                                         gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                               <  1:632607/69‑1 (MQ=255)
                                           tACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc                            >  1:2892101/1‑70 (MQ=255)
                                            aCTCAGGTACTGATCCGACCAGGTACATACGCAGCTGc                                                           >  1:2568680/1‑38 (MQ=255)
                                             cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcccc                         <  1:2316874/71‑1 (MQ=255)
                                             cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                          <  1:315582/70‑1 (MQ=255)
                                             cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                          <  1:1413914/70‑1 (MQ=255)
                                             cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                          <  1:3493113/70‑1 (MQ=255)
                                              tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGTTGCGTCCGGTGAt                                      <  1:2296213/57‑1 (MQ=255)
                                              tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGAt                                      <  1:2331357/57‑1 (MQ=255)
                                              tCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                                   <  1:2933217/60‑1 (MQ=255)
                                               cAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGa                       >  1:3094513/1‑71 (MQ=255)
                                                aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGat                      >  1:1626206/1‑70 (MQ=255)
                                                aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGat                      >  1:1277992/1‑70 (MQ=255)
                                                aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                      >  1:3257161/1‑71 (MQ=255)
                                                  gTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGtgt                                 <  1:289122/58‑3 (MQ=255)
                                                  gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCt                                                  <  1:2413744/41‑1 (MQ=255)
                                                  gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                               >  1:2573784/1‑60 (MQ=255)
                                                  gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCa                    <  1:2080786/71‑1 (MQ=255)
                                                   tACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaa                   <  1:3020262/71‑1 (MQ=255)
                                                    aCTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGc                                                >  1:2165715/1‑41 (MQ=255)
                                                     cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa                 <  1:2419635/71‑1 (MQ=255)
                                                     cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                  <  1:70292/70‑1 (MQ=255)
                                                        aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACg              <  1:660131/71‑1 (MQ=255)
                                                         tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCGAAAACgg             <  1:3952017/71‑1 (MQ=255)
                                                             cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                          <  1:1866897/54‑1 (MQ=255)
                                                             cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCaaa         <  1:3843591/71‑1 (MQ=255)
                                                             cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCaa          <  1:2235528/70‑1 (MQ=255)
                                                              ccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAAt        >  1:2956086/1‑71 (MQ=255)
                                                                aGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTATTCATCCCCGAGCAAAAACGGCAAATTg      <  1:1975554/71‑1 (MQ=255)
                                                                  gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTc    >  1:2704787/1‑71 (MQ=255)
                                                                    aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGc  >  1:2167269/1‑71 (MQ=255)
                                                                      |                                                                    
ACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGC  >  minE/2570742‑2570880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑