BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,571,166	T→C	18.9%	V357V (GTT→GTC)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,571,166	0	T	C	18.9%	273.8 / 55.3	127	V357V (GTT→GTC)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (52/51); new base C (16/8); total (68/59)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 132 total aligned reads displayed.

TGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGG  >  minE/2571097‑2571236

tgatATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGttt                                                                       <  1:1883885/71‑1 (MQ=255)

 gatATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGttt                                                                       >  1:1149055/1‑70 (MQ=255)

    atCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCg                                                                     <  1:1803250/69‑1 (MQ=255)

    atCGATGCCTTACACGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt                                                                   >  1:2142560/1‑71 (MQ=255)

     tCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt                                                                   <  1:1753831/70‑1 (MQ=255)

       gATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGa                                                                 <  1:1608881/70‑1 (MQ=255)

            cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGt                                                                   <  1:2898723/63‑1 (MQ=255)

            cTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa                                                           <  1:707775/71‑1 (MQ=255)

             ttACCCGACGGCATCTACGCCCACGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa                                                           >  1:3533788/1‑70 (MQ=255)

              tACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa                                                           >  1:3348979/1‑69 (MQ=255)

               aCCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGaa                                                          <  1:2904217/69‑1 (MQ=255)

               aCCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAg                                                        >  1:21810/1‑71 (MQ=255)

                cccGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGTTGAAAg                                                        <  1:508836/70‑1 (MQ=255)

                  cGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGtgc                                                     >  1:2823678/1‑71 (MQ=255)

                  agACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgc                                                     >  1:3886495/2‑71 (MQ=255)

                   gACGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgc                                                     >  1:1792418/1‑70 (MQ=255)

                   gACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGtgct                                                    >  1:3001520/1‑71 (MQ=255)

                     cGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgctat                                                  <  1:2438973/71‑3 (MQ=255)

                     cGGTATTTACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGtgctat                                                  <  1:632030/71‑3 (MQ=255)

                     cGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa                                                           <  1:980118/62‑1 (MQ=255)

                       gCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTgctgct                                                 <  1:203597/70‑1 (MQ=255)

                       gCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGCtctgct                                                 <  1:30981/70‑1 (MQ=255)

                        cATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTgctgct                                                 >  1:2786236/1‑69 (MQ=255)

                        cATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTaa                                               <  1:1314144/71‑1 (MQ=255)

                         aTCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa                                                           <  1:2312843/58‑1 (MQ=255)

                          tttACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGt                                                       >  1:1605840/3‑61 (MQ=255)

                          tttACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGt                                                       >  1:384371/3‑61 (MQ=255)

                            tACGCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGAt                                                                >  1:1667617/1‑50 (MQ=255)

                            tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGtgctg                                                   >  1:1551595/1‑63 (MQ=255)

                            tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTa                                                <  1:1914890/66‑1 (MQ=255)

                              cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAAt                                           <  1:1875898/69‑1 (MQ=255)

                              cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATg                                          <  1:453814/70‑1 (MQ=255)

                              cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa                                         >  1:1161956/1‑71 (MQ=255)

                               gCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAc                                        >  1:3320366/1‑71 (MQ=255)

                                cccAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg                                              <  1:2781918/64‑1 (MQ=255)

                                 ccAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                        >  1:3008480/1‑69 (MQ=255)

                                 ccAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                        >  1:1196244/1‑69 (MQ=255)

                                  cAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCaa                                                          <  1:1407586/50‑1 (MQ=255)

                                  cAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAg                                     >  1:2751226/1‑71 (MQ=255)

                                  cAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg                                              <  1:2138075/62‑1 (MQ=255)

                                  cAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg                                              <  1:2218805/62‑1 (MQ=255)

                                   aGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAg                                     >  1:456662/1‑70 (MQ=255)

                                    ggTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                   >  1:1054204/1‑71 (MQ=255)

                                    ggTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                   <  1:3030388/71‑1 (MQ=255)

                                       ggCGGTTTACTCTGATCATTTCAGCCATGTCTCgg                                                                    >  1:3648951/1‑35 (MQ=25)

                                           gTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGc                             <  1:1996898/70‑1 (MQ=255)

                                           gTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGaaa                                                         <  1:247820/42‑1 (MQ=255)

                                           gTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGaaa                                                         <  1:2786197/42‑1 (MQ=255)

                                            tttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGaaa                                                         <  1:3604861/41‑1 (MQ=255)

                                            tttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGa                                                           <  1:3454220/39‑1 (MQ=255)

                                            tttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                           >  1:3660374/1‑71 (MQ=255)

                                             ttACTCCGACCATTTCAGCGATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                           >  1:1965689/1‑70 (MQ=255)

                                             ttACTCCGACCATTTCAGCCATGTTTCGGTGATGc                                                              >  1:1813811/1‑35 (MQ=255)

                                             ttACTCCGACCATTTCAGCCATGTTTCGGTGATGc                                                              >  1:1230345/1‑35 (MQ=255)

                                             ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGaa                                                          >  1:579764/1‑39 (MQ=255)

                                             ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                >  1:3287485/1‑65 (MQ=255)

                                             ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                           >  1:1093774/1‑70 (MQ=255)

                                             ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                           >  1:917966/1‑70 (MQ=255)

                                              tACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAg                                     <  1:1790166/59‑1 (MQ=255)

                                              tACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTa                         >  1:1522456/1‑71 (MQ=255)

                                               aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                        >  1:2720778/1‑71 (MQ=255)

                                               aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                        >  1:1135165/1‑71 (MQ=255)

                                               aCTCCGAACATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATg                              >  1:1763574/1‑65 (MQ=255)

                                                cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                        >  1:397751/1‑54 (MQ=255)

                                                cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                        <  1:3107350/70‑1 (MQ=255)

                                                 tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                            >  1:2466234/1‑49 (MQ=255)

                                                 tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                             <  1:3054275/48‑1 (MQ=255)

                                                 tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                             <  1:3938729/48‑1 (MQ=255)

                                                 tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                             <  1:1765018/48‑1 (MQ=255)

                                                 tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                             <  1:3016000/48‑1 (MQ=255)

                                                  ccGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCtt                     >  1:3791928/1‑71 (MQ=255)

                                                   cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGtgct                                                    >  1:287753/1‑39 (MQ=255)

                                                   cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                >  1:911869/1‑59 (MQ=255)

                                                    gatcATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTa                   >  1:1253556/4‑71 (MQ=255)

                                                     aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                        >  1:3422157/1‑49 (MQ=255)

                                                     aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                        >  1:1563447/1‑49 (MQ=255)

                                                      ccATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAtt                 >  1:3805910/1‑71 (MQ=255)

                                                       cATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt                <  1:3093572/71‑1 (MQ=255)

                                                        aTTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTg               <  1:653346/71‑1 (MQ=255)

                                                        aTTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACAAGCTg                                  >  1:1587590/1‑52 (MQ=255)

                                                          ttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAtt                          <  1:1465736/58‑1 (MQ=255)

                                                          ttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGa             >  1:2111540/1‑71 (MQ=255)

                                                          ttCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAc                                        >  1:64608/1‑44 (MQ=255)

                                                          ttCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAc                                        >  1:2485515/1‑44 (MQ=255)

                                                           tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                        >  1:2358875/1‑43 (MQ=255)

                                                           tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAg                                     <  1:2489464/46‑1 (MQ=255)

                                                           tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAg                                     <  1:3544517/46‑1 (MQ=255)

                                                           tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGAt            >  1:2770059/1‑71 (MQ=255)

                                                             aGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                   >  1:1398188/1‑46 (MQ=255)

                                                             aGCCATGTTT‑GGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt         <  1:326249/71‑1 (MQ=255)

                                                               ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt        <  1:3915486/71‑1 (MQ=255)

                                                                cATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtt        >  1:3698512/1‑70 (MQ=255)

                                                                cATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtt        >  1:77267/1‑70 (MQ=255)

                                                                 aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTa                         >  1:3891288/1‑52 (MQ=255)

                                                                 aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                   >  1:407296/1‑58 (MQ=255)

                                                                 aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTga      <  1:1162533/71‑1 (MQ=255)

                                                                 aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt        >  1:3023694/1‑69 (MQ=255)

                                                                  tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTgg              <  1:3492791/62‑1 (MQ=255)

                                                                  tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTgag     <  1:1721879/71‑1 (MQ=255)

                                                                   gTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagg    >  1:1719969/1‑71 (MQ=255)

TGATATCGATGCCTTACCCGACGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGG  >  minE/2571097‑2571236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`