BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,571,187	G→A	16.6%	L364L (CTG→CTA)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,571,187	0	G	A	16.6%	228.2 / 33.6	96	L364L (CTG→CTA)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (43/37); new base A (11/5); total (54/42)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

CGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGAT  >  minE/2571118‑2571248

cGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGtgctgc                                                              >  1:652245/1‑71 (MQ=255)

  gCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGCtctgct                                                             <  1:30981/70‑1 (MQ=255)

   cATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTgctgct                                                             >  1:2786236/1‑69 (MQ=255)

   cATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTaa                                                           <  1:1314144/71‑1 (MQ=255)

       tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGtgctg                                                               >  1:1551595/1‑63 (MQ=255)

       tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTa                                                            <  1:1914890/66‑1 (MQ=255)

         cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAAt                                                       <  1:1875898/69‑1 (MQ=255)

         cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATg                                                      <  1:453814/70‑1 (MQ=255)

         cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa                                                     >  1:1161956/1‑71 (MQ=255)

          gCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAc                                                    >  1:3320366/1‑71 (MQ=255)

           cccAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg                                                          <  1:2781918/64‑1 (MQ=255)

            ccAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                    >  1:1196244/1‑69 (MQ=255)

            ccAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                    >  1:3008480/1‑69 (MQ=255)

             cAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAg                                                 >  1:2751226/1‑71 (MQ=255)

             cAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg                                                          <  1:2138075/62‑1 (MQ=255)

             cAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg                                                          <  1:2218805/62‑1 (MQ=255)

              aGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAg                                                 >  1:456662/1‑70 (MQ=255)

               ggTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                               <  1:3030388/71‑1 (MQ=255)

               ggTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                               >  1:1054204/1‑71 (MQ=255)

                      gTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCt                                               <  1:2082187/64‑1 (MQ=255)

                      gTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGc                                         <  1:1996898/70‑1 (MQ=255)

                       tttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                       >  1:3660374/1‑71 (MQ=255)

                        ttACTCCGACCATTTCAGCGATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                       >  1:1965689/1‑70 (MQ=255)

                        ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                            >  1:3287485/1‑65 (MQ=255)

                        ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                       >  1:1093774/1‑70 (MQ=255)

                        ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                       >  1:917966/1‑70 (MQ=255)

                         tACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAg                                                 <  1:1790166/59‑1 (MQ=255)

                         tACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTa                                     >  1:1522456/1‑71 (MQ=255)

                          aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                    >  1:2720778/1‑71 (MQ=255)

                          aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                    >  1:1135165/1‑71 (MQ=255)

                          aCTCCGAACATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATg                                          >  1:1763574/1‑65 (MQ=255)

                           cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                    >  1:397751/1‑54 (MQ=255)

                           cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCac                                                 <  1:1950544/57‑2 (MQ=255)

                           cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                            >  1:216338/1‑62 (MQ=255)

                           cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                    <  1:3107350/70‑1 (MQ=255)

                           cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATc                                   <  1:2534019/71‑1 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                        >  1:1575588/1‑49 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                        >  1:3063680/1‑49 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                        >  1:2466234/1‑49 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                         <  1:1765018/48‑1 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                         <  1:3016000/48‑1 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                         <  1:3054275/48‑1 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                         <  1:3938729/48‑1 (MQ=255)

                             ccGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCtt                                 >  1:3791928/1‑71 (MQ=255)

                              cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                            >  1:911869/1‑59 (MQ=255)

                               gatcATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTa                               >  1:1253556/4‑71 (MQ=255)

                               gACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                               <  1:3872986/71‑1 (MQ=255)

                                aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATg                                                      <  1:1630614/47‑1 (MQ=255)

                                aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                    >  1:3422157/1‑49 (MQ=255)

                                aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                    >  1:1563447/1‑49 (MQ=255)

                                 ccATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAtt                             >  1:3805910/1‑71 (MQ=255)

                                  cATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt                            <  1:3483817/71‑1 (MQ=255)

                                  cATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt                            <  1:3093572/71‑1 (MQ=255)

                                   aTTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTg                           <  1:653346/71‑1 (MQ=255)

                                   aTTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACAAGCTg                                              >  1:1587590/1‑52 (MQ=255)

                                     ttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAtt                                      <  1:1465736/58‑1 (MQ=255)

                                     ttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGa                         >  1:2111540/1‑71 (MQ=255)

                                     ttCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAc                                                    >  1:64608/1‑44 (MQ=255)

                                     ttCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAc                                                    >  1:2485515/1‑44 (MQ=255)

                                      tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                    >  1:2358875/1‑43 (MQ=255)

                                      tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAg                                                 <  1:3544517/46‑1 (MQ=255)

                                      tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAg                                                 <  1:2489464/46‑1 (MQ=255)

                                      tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGAt                        >  1:2770059/1‑71 (MQ=255)

                                       cAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                    >  1:1161245/1‑58 (MQ=255)

                                       cAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                    >  1:894796/1‑58 (MQ=255)

                                        aGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                               >  1:1398188/1‑46 (MQ=255)

                                          ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                    <  1:3915486/71‑1 (MQ=255)

                                          ccATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCa                      <  1:1846212/69‑1 (MQ=255)

                                           cATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtt                    >  1:77267/1‑70 (MQ=255)

                                           cATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtt                    >  1:3698512/1‑70 (MQ=255)

                                            aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTa                                     >  1:3891288/1‑52 (MQ=255)

                                            aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                               >  1:407296/1‑58 (MQ=255)

                                            aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTga                  <  1:1162533/71‑1 (MQ=255)

                                            aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                    >  1:1513791/1‑69 (MQ=255)

                                            aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                    >  1:3023694/1‑69 (MQ=255)

                                             tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTgg                          <  1:3492791/62‑1 (MQ=255)

                                             tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTgag                 <  1:1721879/71‑1 (MQ=255)

                                             tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgag                 <  1:2476756/71‑1 (MQ=255)

                                              gtctCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAt                     >  1:2826972/4‑66 (MQ=255)

                                              gTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagg                >  1:1719969/1‑71 (MQ=255)

                                                ttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                       >  1:3599276/1‑46 (MQ=255)

                                                ttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg              >  1:3113909/1‑71 (MQ=255)

                                                 tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt                     >  1:2674656/1‑63 (MQ=255)

                                                 tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg              <  1:2331410/70‑1 (MQ=255)

                                                 tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg              >  1:1621007/1‑70 (MQ=255)

                                                 tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg              >  1:1591226/1‑70 (MQ=255)

                                                 tCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAt                              <  1:2896902/54‑1 (MQ=255)

                                                   ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTTGATCATTgagggggag           <  1:1659192/71‑1 (MQ=255)

                                                   ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggggag           <  1:2609564/71‑1 (MQ=255)

                                                   ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggga            <  1:3515525/70‑1 (MQ=255)

                                                        tGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGagggggaggggg       >  1:3676318/1‑70 (MQ=255)

                                                        tGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGagggggaggggg       >  1:2541047/1‑70 (MQ=255)

                                                         gcgcAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAtct                            >  1:1282629/1‑46 (MQ=255)

                                                           ggAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                               <  1:1134184/43‑1 (MQ=255)

                                                            gAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTGAGGGGGAGGGGGGAGAt  <  1:965757/71‑1 (MQ=255)

                                                            gAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagg                <  1:3503709/57‑1 (MQ=255)

                                                             aaaGTGCTGCTAAGAATGACCAGCTTGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGAt  >  1:3500077/1‑70 (MQ=255)

                                                              aaGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg              >  1:737039/1‑57 (MQ=255)

                                                                   ctgctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggga            >  1:71673/1‑54 (MQ=255)

                                                                   ctgctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgag                 >  1:2333096/1‑49 (MQ=255)

CGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGAT  >  minE/2571118‑2571248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`