BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,571,188	C→T	16.6%	L365L (CTA→TTA)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,571,188	0	C	T	16.6%	229.2 / 32.0	96	L365L (CTA→TTA)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (43/37); new base T (11/5); total (54/42)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

CGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTT  >  minE/2571118‑2571258

cGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGtgctgc                                                                        >  1:652245/1‑71 (MQ=255)

  gCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTgctgct                                                                       <  1:203597/70‑1 (MQ=255)

  gCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGCtctgct                                                                       <  1:30981/70‑1 (MQ=255)

   cATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTgctgct                                                                       >  1:2786236/1‑69 (MQ=255)

   cATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTaa                                                                     <  1:1314144/71‑1 (MQ=255)

       tACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTa                                                                      <  1:1914890/66‑1 (MQ=255)

         cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAAt                                                                 <  1:1875898/69‑1 (MQ=255)

         cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATg                                                                <  1:453814/70‑1 (MQ=255)

         cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa                                                               >  1:1161956/1‑71 (MQ=255)

          gCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAc                                                              >  1:3320366/1‑71 (MQ=255)

           cccAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg                                                                    <  1:2781918/64‑1 (MQ=255)

            ccAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                              >  1:1196244/1‑69 (MQ=255)

            ccAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                              >  1:3008480/1‑69 (MQ=255)

             cAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAg                                                           >  1:2751226/1‑71 (MQ=255)

             cAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg                                                                    <  1:2218805/62‑1 (MQ=255)

             cAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAg                                                                    <  1:2138075/62‑1 (MQ=255)

              aGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAg                                                           >  1:456662/1‑70 (MQ=255)

               ggTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                                         >  1:1054204/1‑71 (MQ=255)

                      gTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCt                                                         <  1:2082187/64‑1 (MQ=255)

                      gTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGc                                                   <  1:1996898/70‑1 (MQ=255)

                       tttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                 >  1:3660374/1‑71 (MQ=255)

                        ttACTCCGACCATTTCAGCGATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                 >  1:1965689/1‑70 (MQ=255)

                        ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                      >  1:3287485/1‑65 (MQ=255)

                        ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                 >  1:917966/1‑70 (MQ=255)

                        ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                 >  1:1093774/1‑70 (MQ=255)

                         tACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAg                                                           <  1:1790166/59‑1 (MQ=255)

                         tACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTa                                               >  1:1522456/1‑71 (MQ=255)

                          aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                              >  1:2720778/1‑71 (MQ=255)

                          aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                              >  1:1135165/1‑71 (MQ=255)

                          aCTCCGAACATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATg                                                    >  1:1763574/1‑65 (MQ=255)

                           cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                              >  1:397751/1‑54 (MQ=255)

                           cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCac                                                           <  1:1950544/57‑2 (MQ=255)

                           cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                      >  1:216338/1‑62 (MQ=255)

                           cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                              <  1:3107350/70‑1 (MQ=255)

                           cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATc                                             <  1:2534019/71‑1 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                                  >  1:3063680/1‑49 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                                  >  1:1575588/1‑49 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                                  >  1:2466234/1‑49 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                                   <  1:1765018/48‑1 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                                   <  1:3938729/48‑1 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                                   <  1:3054275/48‑1 (MQ=255)

                            tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                                   <  1:3016000/48‑1 (MQ=255)

                             ccGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCtt                                           >  1:3791928/1‑71 (MQ=255)

                              cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                      >  1:911869/1‑59 (MQ=255)

                               gatcATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTa                                         >  1:1253556/4‑71 (MQ=255)

                               gACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                         <  1:3872986/71‑1 (MQ=255)

                                aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATg                                                                <  1:1630614/47‑1 (MQ=255)

                                aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                              >  1:3422157/1‑49 (MQ=255)

                                aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                              >  1:1563447/1‑49 (MQ=255)

                                 ccATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAtt                                       >  1:3805910/1‑71 (MQ=255)

                                  cATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt                                      <  1:3483817/71‑1 (MQ=255)

                                  cATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt                                      <  1:3093572/71‑1 (MQ=255)

                                   aTTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTg                                     <  1:653346/71‑1 (MQ=255)

                                   aTTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACAAGCTg                                                        >  1:1587590/1‑52 (MQ=255)

                                     ttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAtt                                                <  1:1465736/58‑1 (MQ=255)

                                     ttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGa                                   >  1:2111540/1‑71 (MQ=255)

                                     ttCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAc                                                              >  1:2485515/1‑44 (MQ=255)

                                     ttCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAc                                                              >  1:64608/1‑44 (MQ=255)

                                      tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                              >  1:2358875/1‑43 (MQ=255)

                                      tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAg                                                           <  1:3544517/46‑1 (MQ=255)

                                      tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAg                                                           <  1:2489464/46‑1 (MQ=255)

                                      tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGAt                                  >  1:2770059/1‑71 (MQ=255)

                                       cAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                              >  1:1161245/1‑58 (MQ=255)

                                       cAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                              >  1:894796/1‑58 (MQ=255)

                                        aGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                                         >  1:1398188/1‑46 (MQ=255)

                                        aGCCATGTTT‑GGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt                               <  1:326249/71‑1 (MQ=255)

                                          ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                              <  1:3915486/71‑1 (MQ=255)

                                          ccATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCa                                <  1:1846212/69‑1 (MQ=255)

                                           cATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtt                              >  1:77267/1‑70 (MQ=255)

                                            aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTa                                               >  1:3891288/1‑52 (MQ=255)

                                            aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                         >  1:407296/1‑58 (MQ=255)

                                            aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTga                            <  1:1162533/71‑1 (MQ=255)

                                            aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                              >  1:3023694/1‑69 (MQ=255)

                                            aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                              >  1:1513791/1‑69 (MQ=255)

                                             tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTgg                                    <  1:3492791/62‑1 (MQ=255)

                                             tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTgag                           <  1:1721879/71‑1 (MQ=255)

                                             tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgag                           <  1:2476756/71‑1 (MQ=255)

                                              gtctCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAt                               >  1:2826972/4‑66 (MQ=255)

                                              gTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagg                          >  1:1719969/1‑71 (MQ=255)

                                                ttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                 >  1:3599276/1‑46 (MQ=255)

                                                ttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                        >  1:3113909/1‑71 (MQ=255)

                                                 tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt                               >  1:2674656/1‑63 (MQ=255)

                                                 tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                        <  1:2331410/70‑1 (MQ=255)

                                                 tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                        >  1:1591226/1‑70 (MQ=255)

                                                 tCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAt                                        <  1:2896902/54‑1 (MQ=255)

                                                   ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTTGATCATTgagggggag                     <  1:1659192/71‑1 (MQ=255)

                                                   ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggggag                     <  1:2609564/71‑1 (MQ=255)

                                                   ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggga                      <  1:3515525/70‑1 (MQ=255)

                                                        tGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGagggggaggggg                 >  1:3676318/1‑70 (MQ=255)

                                                        tGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGagggggaggggg                 >  1:2541047/1‑70 (MQ=255)

                                                         gcgcAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAtct                                      >  1:1282629/1‑46 (MQ=255)

                                                           ggAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                         <  1:1134184/43‑1 (MQ=255)

                                                            gAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTGAGGGGGAGGGGGGAGAt            <  1:965757/71‑1 (MQ=255)

                                                            gAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagg                          <  1:3503709/57‑1 (MQ=255)

                                                             aaaGTGCTGCTAAGAATGACCAGCTTGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGAt            >  1:3500077/1‑70 (MQ=255)

                                                              aaGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                        >  1:737039/1‑57 (MQ=255)

                                                                   ctgctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggga                      >  1:71673/1‑54 (MQ=255)

                                                                      ctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTGAGGGGGAGGGGGGAGATTATGGAAc    <  1:1342200/69‑1 (MQ=255)

                                                                      ctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATgg       >  1:2710222/1‑66 (MQ=255)

                                                                      ctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACtt  <  1:3279660/71‑1 (MQ=255)

CGGCATCTACGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTT  >  minE/2571118‑2571258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`