Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,571,193 A→C 17.4% R366S (AGA→AGC) ‡ yibH → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,571,1930AC17.4% 163.1 / 43.6 92R366S (AGA→AGC) ‡yibHhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (46/30);  new base C (11/5);  total (57/35)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCT  >  minE/2571127‑2571262
                                                                  |                                                                     
cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAAt                                                                     <  1:1875898/69‑1 (MQ=255)
cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATg                                                                    <  1:453814/70‑1 (MQ=255)
cGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGa                                                                   >  1:1161956/1‑71 (MQ=255)
 gCCCAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAc                                                                  >  1:3320366/1‑71 (MQ=255)
   ccAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                                  >  1:3008480/1‑69 (MQ=255)
   ccAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                                  >  1:1196244/1‑69 (MQ=255)
    cAGGTGGCGGTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAg                                                               >  1:2751226/1‑71 (MQ=255)
     aGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAg                                                               >  1:456662/1‑70 (MQ=255)
      ggTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                                             <  1:3030388/71‑1 (MQ=255)
      ggTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                                             >  1:1054204/1‑71 (MQ=255)
             gTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCt                                                             <  1:2082187/64‑1 (MQ=255)
             gTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGc                                                       <  1:1996898/70‑1 (MQ=255)
              tttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                     >  1:3660374/1‑71 (MQ=255)
               ttACTCCGACCATTTCAGCGATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                     >  1:1965689/1‑70 (MQ=255)
               ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                          >  1:3287485/1‑65 (MQ=255)
               ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                     >  1:1093774/1‑70 (MQ=255)
               ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                     >  1:917966/1‑70 (MQ=255)
                tACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAg                                                               <  1:1790166/59‑1 (MQ=255)
                tACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTa                                                   >  1:1522456/1‑71 (MQ=255)
                 aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                  >  1:1135165/1‑71 (MQ=255)
                 aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                  >  1:2720778/1‑71 (MQ=255)
                 aCTCCGAACATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATg                                                        >  1:1763574/1‑65 (MQ=255)
                  cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                                  >  1:397751/1‑54 (MQ=255)
                  cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCac                                                               <  1:1950544/57‑2 (MQ=255)
                  cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                          >  1:216338/1‑62 (MQ=255)
                  cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                  <  1:3107350/70‑1 (MQ=255)
                  cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATc                                                 <  1:2534019/71‑1 (MQ=255)
                   tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                                      >  1:2466234/1‑49 (MQ=255)
                   tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                                      >  1:3063680/1‑49 (MQ=255)
                   tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGaa                                                                      >  1:1575588/1‑49 (MQ=255)
                   tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                                       <  1:1765018/48‑1 (MQ=255)
                   tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                                       <  1:3054275/48‑1 (MQ=255)
                   tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                                       <  1:3938729/48‑1 (MQ=255)
                   tCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGa                                                                       <  1:3016000/48‑1 (MQ=255)
                    ccGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCtt                                               >  1:3791928/1‑71 (MQ=255)
                     cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                          >  1:911869/1‑59 (MQ=255)
                      gatcATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTa                                             >  1:1253556/4‑71 (MQ=255)
                      gACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                             <  1:3872986/71‑1 (MQ=255)
                       aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATg                                                                    <  1:1630614/47‑1 (MQ=255)
                       aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                                  >  1:1563447/1‑49 (MQ=255)
                       aCCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                                  >  1:3422157/1‑49 (MQ=255)
                        ccATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAtt                                           >  1:3805910/1‑71 (MQ=255)
                         cATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt                                          <  1:3483817/71‑1 (MQ=255)
                         cATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt                                          <  1:3093572/71‑1 (MQ=255)
                          aTTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTg                                         <  1:653346/71‑1 (MQ=255)
                          aTTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACAAGCTg                                                            >  1:1587590/1‑52 (MQ=255)
                            ttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAtt                                                    <  1:1465736/58‑1 (MQ=255)
                            ttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGa                                       >  1:2111540/1‑71 (MQ=255)
                            ttCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAc                                                                  >  1:2485515/1‑44 (MQ=255)
                            ttCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGAc                                                                  >  1:64608/1‑44 (MQ=255)
                             tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGAc                                                                  >  1:2358875/1‑43 (MQ=255)
                             tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAg                                                               <  1:2489464/46‑1 (MQ=255)
                             tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAg                                                               <  1:3544517/46‑1 (MQ=255)
                             tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGAt                                      >  1:2770059/1‑71 (MQ=255)
                              cAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                  >  1:894796/1‑58 (MQ=255)
                              cAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                  >  1:1161245/1‑58 (MQ=255)
                               aGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCt                                                             >  1:1398188/1‑46 (MQ=255)
                               aGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCa                                    <  1:999983/71‑1 (MQ=255)
                               aGCCATGTTT‑GGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt                                   <  1:326249/71‑1 (MQ=255)
                                 ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                                  <  1:3915486/71‑1 (MQ=255)
                                 ccATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCa                                    <  1:1846212/69‑1 (MQ=255)
                                  cATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtt                                  >  1:3698512/1‑70 (MQ=255)
                                  cATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtt                                  >  1:77267/1‑70 (MQ=255)
                                   aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTa                                                   >  1:3891288/1‑52 (MQ=255)
                                   aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                             >  1:407296/1‑58 (MQ=255)
                                   aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTga                                <  1:1162533/71‑1 (MQ=255)
                                   aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                                  >  1:1513791/1‑69 (MQ=255)
                                   aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                                  >  1:3023694/1‑69 (MQ=255)
                                    tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTgg                                        <  1:3492791/62‑1 (MQ=255)
                                    tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTgag                               <  1:1721879/71‑1 (MQ=255)
                                    tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgag                               <  1:2476756/71‑1 (MQ=255)
                                     gtctCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAt                                   >  1:2826972/4‑66 (MQ=255)
                                     gTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagg                              >  1:1719969/1‑71 (MQ=255)
                                       ttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                     >  1:3599276/1‑46 (MQ=255)
                                       ttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                            >  1:3113909/1‑71 (MQ=255)
                                        tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt                                   >  1:2674656/1‑63 (MQ=255)
                                        tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                            >  1:1591226/1‑70 (MQ=255)
                                        tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                            >  1:1621007/1‑70 (MQ=255)
                                        tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                            <  1:2331410/70‑1 (MQ=255)
                                        tCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAt                                            <  1:2896902/54‑1 (MQ=255)
                                          ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTTGATCATTgagggggag                         <  1:1659192/71‑1 (MQ=255)
                                          ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggggag                         <  1:2609564/71‑1 (MQ=255)
                                          ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggga                          <  1:3515525/70‑1 (MQ=255)
                                               tGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGagggggaggggg                     >  1:3676318/1‑70 (MQ=255)
                                               tGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGagggggaggggg                     >  1:2541047/1‑70 (MQ=255)
                                                gcgcAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAtct                                          >  1:1282629/1‑46 (MQ=255)
                                                  ggAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                             <  1:1134184/43‑1 (MQ=255)
                                                   gAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTGAGGGGGAGGGGGGAGAt                <  1:965757/71‑1 (MQ=255)
                                                   gAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagg                              <  1:3503709/57‑1 (MQ=255)
                                                    aaaGTGCTGCTAAGAATGACCAGCTTGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGAt                >  1:3500077/1‑70 (MQ=255)
                                                     aaGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                            >  1:737039/1‑57 (MQ=255)
                                                          ctgctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggga                          >  1:71673/1‑54 (MQ=255)
                                                          ctgctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgag                               >  1:2333096/1‑49 (MQ=255)
                                                             ctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTGAGGGGGAGGGGGGAGATTATGGAAc        <  1:1342200/69‑1 (MQ=255)
                                                             ctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATgg           >  1:2710222/1‑66 (MQ=255)
                                                             ctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACtt      <  1:3279660/71‑1 (MQ=255)
                                                               aaGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggggaggg                       >  1:1532508/1‑52 (MQ=255)
                                                               aaGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTg    >  1:1522160/1‑71 (MQ=255)
                                                                 gAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCt  >  1:2036300/1‑71 (MQ=255)
                                                                  |                                                                     
CGCCCAGGTGGCGGTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCT  >  minE/2571127‑2571262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑