Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,571,206	A→G	12.3%	M371V (ATG→GTG)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,571,206	0	A	G	12.3%	189.8 / 16.1	73	M371V (ATG→GTG)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (39/25);  new base G (6/3);  total (45/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTGTAAGGAC  >  minE/2571140‑2571274                                                                   |                                                                     gTTTACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGc                                                                   <  1:1996898/70‑1 (MQ=255)  tttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                                 >  1:3660374/1‑71 (MQ=255)   ttACTCCGACCATTTCAGCGATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                                 >  1:1965689/1‑70 (MQ=255)   ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                                      >  1:3287485/1‑65 (MQ=255)   ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                                 >  1:1093774/1‑70 (MQ=255)   ttACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                                 >  1:917966/1‑70 (MQ=255)    tACTCTGATCATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTa                                                               >  1:1522456/1‑71 (MQ=255)     aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                              >  1:1135165/1‑71 (MQ=255)     aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                              >  1:2720778/1‑71 (MQ=255)     aCTCCGAACATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATg                                                                    >  1:1763574/1‑65 (MQ=255)      cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                                      >  1:216338/1‑62 (MQ=255)      cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                              <  1:3107350/70‑1 (MQ=255)      cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATc                                                             <  1:2534019/71‑1 (MQ=255)        ccGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCtt                                                           >  1:3791928/1‑71 (MQ=255)         cGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGa                                                                      >  1:911869/1‑59 (MQ=255)          gatcATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTa                                                         >  1:1253556/4‑71 (MQ=255)          gACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                                         <  1:3872986/71‑1 (MQ=255)            ccATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAtt                                                       >  1:3805910/1‑71 (MQ=255)             cATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt                                                      <  1:3483817/71‑1 (MQ=255)             cATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt                                                      <  1:3093572/71‑1 (MQ=255)              aTTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTg                                                     <  1:653346/71‑1 (MQ=255)                ttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAtt                                                                <  1:1465736/58‑1 (MQ=255)                ttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGa                                                   >  1:2111540/1‑71 (MQ=255)                 tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGAt                                                  >  1:2770059/1‑71 (MQ=255)                  cAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                              >  1:894796/1‑58 (MQ=255)                  cAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                              >  1:1161245/1‑58 (MQ=255)                   aGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCa                                                <  1:999983/71‑1 (MQ=255)                   aGCCATGTTT‑GGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt                                               <  1:326249/71‑1 (MQ=255)                     ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                                              <  1:3915486/71‑1 (MQ=255)                     ccATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCa                                                <  1:1846212/69‑1 (MQ=255)                      cATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtt                                              >  1:3698512/1‑70 (MQ=255)                      cATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtt                                              >  1:77267/1‑70 (MQ=255)                       aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTa                                                               >  1:3891288/1‑52 (MQ=255)                       aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                                         >  1:407296/1‑58 (MQ=255)                       aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTga                                            <  1:1162533/71‑1 (MQ=255)                       aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                                              >  1:3023694/1‑69 (MQ=255)                       aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                                              >  1:1513791/1‑69 (MQ=255)                        tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTgg                                                    <  1:3492791/62‑1 (MQ=255)                        tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTgag                                           <  1:1721879/71‑1 (MQ=255)                        tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgag                                           <  1:2476756/71‑1 (MQ=255)                         gtctCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAt                                               >  1:2826972/4‑66 (MQ=255)                         gTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagg                                          >  1:1719969/1‑71 (MQ=255)                           ttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCAt                                                                 >  1:3599276/1‑46 (MQ=255)                           ttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                                        >  1:3113909/1‑71 (MQ=255)                            tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt                                               >  1:2674656/1‑63 (MQ=255)                            tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                                        >  1:1591226/1‑70 (MQ=255)                            tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                                        <  1:2331410/70‑1 (MQ=255)                            tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                                        >  1:1621007/1‑70 (MQ=255)                            tCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAt                                                        <  1:2896902/54‑1 (MQ=255)                              ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTTGATCATTgagggggag                                     <  1:1659192/71‑1 (MQ=255)                              ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggggag                                     <  1:2609564/71‑1 (MQ=255)                              ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggga                                      <  1:3515525/70‑1 (MQ=255)                                   tGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGagggggaggggg                                 >  1:2541047/1‑70 (MQ=255)                                   tGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGagggggaggggg                                 >  1:3676318/1‑70 (MQ=255)                                    gcgcAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAtct                                                      >  1:1282629/1‑46 (MQ=255)                                      ggAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                                         <  1:1134184/43‑1 (MQ=255)                                       gAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTGAGGGGGAGGGGGGAGAt                            <  1:965757/71‑1 (MQ=255)                                       gAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagg                                          <  1:3503709/57‑1 (MQ=255)                                        aaaGTGCTGCTAAGAATGACCAGCTTGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGAt                            >  1:3500077/1‑70 (MQ=255)                                         aaGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                                        >  1:737039/1‑57 (MQ=255)                                              ctgctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggga                                      >  1:71673/1‑54 (MQ=255)                                              ctgctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgag                                           >  1:2333096/1‑49 (MQ=255)                                                 ctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTGAGGGGGAGGGGGGAGATTATGGAAc                    <  1:1342200/69‑1 (MQ=255)                                                 ctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATgg                       >  1:2710222/1‑66 (MQ=255)                                                 ctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACtt                  <  1:3279660/71‑1 (MQ=255)                                                   aaGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggggaggg                                   >  1:1532508/1‑52 (MQ=255)                                                   aaGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTg                >  1:1522160/1‑71 (MQ=255)                                                     gAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCt              >  1:2036300/1‑71 (MQ=255)                                                       aTGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTcc            >  1:2325304/1‑71 (MQ=255)                                                        tGACCAGCTGGATGCATTATCTTTATTTGGTTCATTga                                            <  1:98407/38‑1 (MQ=255)                                                        tGACCAGCTGGATGCATTATCTTTATTTGGTTCATTga                                            <  1:994093/38‑1 (MQ=255)                                                        tGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAgggggaggggggag                              >  1:232550/1‑52 (MQ=255)                                                        tGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCt           >  1:3083405/1‑71 (MQ=255)                                                          aCCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTg         >  1:1715649/1‑71 (MQ=255)                                                                 ggATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTg                >  1:2817668/1‑57 (MQ=255)                                                                  gATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTGTAAGGAc  >  1:1655067/1‑70 (MQ=255)                                                                   aTGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTGTAAGGAc  >  1:1767080/1‑69 (MQ=255)                                                                   |                                                                     GTTTACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTGTAAGGAC  >  minE/2571140‑2571274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑