Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,571,214	T→C	10.8%	Y373Y (TAT→TAC)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,571,214	0	T	C	10.8%	175.8 / 10.8	65	Y373Y (TAT→TAC)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/24);  new base C (5/2);  total (39/26)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTGTAAGGACTAATTATTTT  >  minE/2571144‑2571284                                                                       |                                                                       aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                                        >  1:1135165/1‑71 (MQ=255) aCTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                                        >  1:2720778/1‑71 (MQ=255)  cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                                        <  1:3107350/70‑1 (MQ=255)  cTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATc                                                                       <  1:2534019/71‑1 (MQ=255)    ccGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCtt                                                                     >  1:3791928/1‑71 (MQ=255)      gatcATTTCAGCCATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTa                                                                   >  1:1253556/4‑71 (MQ=255)      gACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                                                   <  1:3872986/71‑1 (MQ=255)        ccATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAtt                                                                 >  1:3805910/1‑71 (MQ=255)         cATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt                                                                <  1:3483817/71‑1 (MQ=255)         cATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTAttt                                                                <  1:3093572/71‑1 (MQ=255)          aTTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTg                                                               <  1:653346/71‑1 (MQ=255)            ttCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGa                                                             >  1:2111540/1‑71 (MQ=255)             tCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGAt                                                            >  1:2770059/1‑71 (MQ=255)              cAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                                        >  1:894796/1‑58 (MQ=255)              cAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTAt                                                                        >  1:1161245/1‑58 (MQ=255)               aGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCa                                                          <  1:999983/71‑1 (MQ=255)               aGCCATGTTT‑GGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt                                                         <  1:326249/71‑1 (MQ=255)                 ccATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                                                        <  1:3915486/71‑1 (MQ=255)                 ccATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCa                                                          <  1:1846212/69‑1 (MQ=255)                  cATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtt                                                        >  1:77267/1‑70 (MQ=255)                  cATGTCTCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAtt                                                        >  1:3698512/1‑70 (MQ=255)                   aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                                                   >  1:407296/1‑58 (MQ=255)                   aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTga                                                      <  1:1162533/71‑1 (MQ=255)                   aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                                                        >  1:1513791/1‑69 (MQ=255)                   aTGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAtt                                                        >  1:3023694/1‑69 (MQ=255)                    tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTgg                                                              <  1:3492791/62‑1 (MQ=255)                    tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTgag                                                     <  1:1721879/71‑1 (MQ=255)                    tGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgag                                                     <  1:2476756/71‑1 (MQ=255)                     gtctCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTATCTCGATCAt                                                         >  1:2826972/4‑66 (MQ=255)                     gTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagg                                                    >  1:1719969/1‑71 (MQ=255)                       ttCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                                                  >  1:3113909/1‑71 (MQ=255)                        tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCAt                                                         >  1:2674656/1‑63 (MQ=255)                        tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                                                  >  1:1621007/1‑70 (MQ=255)                        tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                                                  <  1:2331410/70‑1 (MQ=255)                        tCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                                                  >  1:1591226/1‑70 (MQ=255)                        tCGGTGATGCGCAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAt                                                                  <  1:2896902/54‑1 (MQ=255)                          ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTTGATCATTgagggggag                                               <  1:1659192/71‑1 (MQ=255)                          ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggggag                                               <  1:2609564/71‑1 (MQ=255)                          ggTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggga                                                <  1:3515525/70‑1 (MQ=255)                               tGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGagggggaggggg                                           >  1:2541047/1‑70 (MQ=255)                               tGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGagggggaggggg                                           >  1:3676318/1‑70 (MQ=255)                                gcgcAAAGTGCTATTACGCATGACCAGCTGGGTGCATTACCTTTAtct                                                                >  1:1282629/1‑46 (MQ=255)                                  ggAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTa                                                                   <  1:1134184/43‑1 (MQ=255)                                   gAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTGAGGGGGAGGGGGGAGAt                                      <  1:965757/71‑1 (MQ=255)                                   gAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagg                                                    <  1:3503709/57‑1 (MQ=255)                                    aaaGTGCTGCTAAGAATGACCAGCTTGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGAt                                      >  1:3500077/1‑70 (MQ=255)                                     aaGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggg                                                  >  1:737039/1‑57 (MQ=255)                                          ctgctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgaggggga                                                >  1:71673/1‑54 (MQ=255)                                          ctgctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgag                                                     >  1:2333096/1‑49 (MQ=255)                                             ctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGTTCATTGAGGGGGAGGGGGGAGATTATGGAAc                              <  1:1342200/69‑1 (MQ=255)                                             ctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATgg                                 >  1:2710222/1‑66 (MQ=255)                                             ctAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACtt                            <  1:3279660/71‑1 (MQ=255)                                               aaGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTgagggggaggg                                             >  1:1532508/1‑52 (MQ=255)                                               aaGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTg                          >  1:1522160/1‑71 (MQ=255)                                                 gAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCt                        >  1:2036300/1‑71 (MQ=255)                                                   aTGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTcc                      >  1:2325304/1‑71 (MQ=255)                                                    tGACCAGCTGGATGCATTATCTTTATTTGGTTCATTga                                                      <  1:98407/38‑1 (MQ=255)                                                    tGACCAGCTGGATGCATTATCTTTATTTGGTTCATTga                                                      <  1:994093/38‑1 (MQ=255)                                                    tGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAgggggaggggggag                                        >  1:232550/1‑52 (MQ=255)                                                    tGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCt                     >  1:3083405/1‑71 (MQ=255)                                                      aCCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTg                   >  1:1715649/1‑71 (MQ=255)                                                             ggATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTg                          >  1:2817668/1‑57 (MQ=255)                                                              gATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTGTAAGGAc            >  1:1655067/1‑70 (MQ=255)                                                               aTGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTGTAAGGAc            >  1:1767080/1‑69 (MQ=255)                                                                tGCATTATCTTTATTTGGTTCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGc                         >  1:1528940/1‑55 (MQ=255)                                                                tGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGgaga                                       >  1:340802/1‑41 (MQ=255)                                                                     tATCTTTATTTGGTTCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTGTAAGGACTAattatt    >  1:402068/1‑71 (MQ=255)                                                                      aTCTTTATTTGGTTCATTGAGGGGGAGGGGGGAGATTa                                    >  1:1101065/1‑38 (MQ=255)                                                                      aTCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGaa                               <  1:502394/43‑1 (MQ=255)                                                                      aTCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTGTAAGGACTAATTAttt   >  1:2972643/1‑71 (MQ=255)                                                                       tCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTGTAAGGACTAATTAtttt  >  1:111600/1‑71 (MQ=255)                                                                       |                                                                       ACTCCGACCATTTCAGCCATGTTTCGGTGATGCGGAAAGTGCTGCTAAGAATGACCAGCTGGATGCATTATCTTTATTTGGATCATTGAGGGGGAGGGGGGAGATTATGGAACTTTGCTCCTTGTAAGGACTAATTATTTT  >  minE/2571144‑2571284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑