Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,571,326 T→C 15.5% intergenic (+94/‑1685) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,571,3260TC15.5% 173.5 / 22.2 71intergenic (+94/‑1685)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/39);  new base C (8/3);  total (29/42)
Fisher's exact test for biased strand distribution p-value = 4.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

CTTTGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGC  >  minE/2571256‑2571392
                                                                       |                                                                   
cTTTGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATAtt                                                                     <  1:3917052/71‑1 (MQ=255)
   tGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTc                                                                    <  1:3390868/69‑1 (MQ=255)
   tGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCa                                                                   >  1:3033259/1‑70 (MQ=255)
   tGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCAt                                                                  >  1:232100/1‑71 (MQ=255)
   tGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCAt                                                                  >  1:1758303/1‑71 (MQ=255)
     cTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTa                                                                <  1:737063/71‑1 (MQ=255)
        cTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCAtt                                                                 <  1:848762/67‑1 (MQ=255)
        cTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCAt                                                                  <  1:2139544/66‑1 (MQ=255)
           gTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCa                                                                   <  1:1386381/62‑1 (MQ=255)
           gTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaa                                                          >  1:450382/1‑71 (MQ=255)
            tAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaa                                                          <  1:921862/70‑1 (MQ=255)
             aaGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaat                                                         <  1:3318810/70‑1 (MQ=255)
              aGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaataa                                                       <  1:2593480/71‑1 (MQ=255)
               ggACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAg                                                      <  1:78859/71‑1 (MQ=255)
                gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                     <  1:1057921/71‑1 (MQ=255)
                gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                     <  1:707929/71‑1 (MQ=255)
                 aCTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                     <  1:707223/70‑1 (MQ=255)
                   tAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATAtt                                                                     <  1:2904910/52‑1 (MQ=255)
                   tAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaa                                                          >  1:3075837/1‑63 (MQ=255)
                     attattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaata                                                        >  1:106744/1‑63 (MQ=255)
                     attattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                <  1:2119564/70‑1 (MQ=255)
                      ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGt                                                             >  1:477513/1‑57 (MQ=255)
                      ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                              <  1:1232140/71‑1 (MQ=255)
                      ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                              <  1:2225942/71‑1 (MQ=255)
                       tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                             >  1:85200/1‑71 (MQ=255)
                       tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                             >  1:418124/1‑71 (MQ=255)
                       tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                             >  1:2182597/1‑71 (MQ=255)
                        attTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                             >  1:2254309/1‑70 (MQ=255)
                         tttttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATg                                            <  1:3533469/70‑1 (MQ=255)
                           tttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAg                                          <  1:2502571/70‑1 (MQ=255)
                             tGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgtg                                        <  1:2389877/70‑1 (MQ=255)
                              gCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgtgt                                       >  1:1360047/1‑70 (MQ=255)
                               cATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTc                                                                    <  1:20619/41‑1 (MQ=255)
                                atatTACTTCATTAGCTTCGATTATTTTTCTTTAATATTCATTATGTTAATAAGtt                                                    >  1:1502246/1‑56 (MQ=255)
                                atatTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTAct                                    <  1:2694549/71‑1 (MQ=255)
                                 tatTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCAt                                                                  <  1:1662302/41‑1 (MQ=255)
                                  atTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaata                                                        <  1:752619/50‑1 (MQ=255)
                                   ttACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGctct                                  <  1:175902/70‑1 (MQ=255)
                                     aCTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCa                                <  1:1985376/70‑1 (MQ=255)
                                     aCTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCa                                <  1:3027968/70‑1 (MQ=255)
                                      cTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaataa                                                       >  1:1194867/1‑47 (MQ=255)
                                      cTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCAtt                              <  1:2485622/71‑1 (MQ=255)
                                         cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                              >  1:1637292/1‑52 (MQ=255)
                                         cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTg                             <  1:2992931/69‑1 (MQ=255)
                                         cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGa                           >  1:2008979/1‑71 (MQ=255)
                                          aTTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGGGTGCTCTCATTGGAt                          <  1:2791507/71‑1 (MQ=255)
                                           ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAg                                                      <  1:2789876/43‑1 (MQ=255)
                                           ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                <  1:1884285/48‑1 (MQ=255)
                                           ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                <  1:909931/48‑1 (MQ=255)
                                             aGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                              >  1:2105731/1‑48 (MQ=255)
                                               cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                     <  1:1410050/71‑1 (MQ=255)
                                               cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                     <  1:1809107/71‑1 (MQ=255)
                                               cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                     <  1:51191/71‑1 (MQ=255)
                                                 tCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgt                                         >  1:1241951/1‑49 (MQ=255)
                                                 tCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgt                                         >  1:2625243/1‑49 (MQ=255)
                                                 gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCt                                                 >  1:1733264/3‑42 (MQ=12)
                                                 gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCTg                                                >  1:2571275/3‑43 (MQ=16)
                                                  cGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATc                  >  1:466854/1‑71 (MQ=255)
                                                   gatgatTTTTCCTTTAATATCCAGCATGTAAAGAAGTTc                                                   >  1:2962167/1‑39 (MQ=9)
                                                    atgatTTTTCTTTAATATTCATTATGTTAATAAGtt                                                    <  1:1363014/36‑1 (MQ=255)
                                                    atgatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCg                <  1:1035998/71‑1 (MQ=255)
                                                     tgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATcc                 >  1:602030/1‑71 (MQ=255)
                                                      gatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc              >  1:334079/1‑71 (MQ=255)
                                                       atTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc              <  1:833991/70‑1 (MQ=255)
                                                            tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc              >  1:1663272/3‑67 (MQ=255)
                                                            tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg           <  1:1699436/68‑1 (MQ=255)
                                                             ccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTga          <  1:2002593/69‑2 (MQ=255)
                                                              cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTACCCGTCTTGATc        <  1:681628/71‑1 (MQ=255)
                                                              atttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc        >  1:1204721/2‑71 (MQ=255)
                                                                ttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTg      >  1:1385251/1‑71 (MQ=255)
                                                                 tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTgt     >  1:3859165/1‑70 (MQ=255)
                                                                    tatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAt                          <  1:2954658/46‑1 (MQ=255)
                                                                    tatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTAACCGTCTTGTTCTGCCGc  <  1:1658843/70‑1 (MQ=255)
                                                                       |                                                                   
CTTTGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGC  >  minE/2571256‑2571392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: