Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,571,329 T→G 17.2% intergenic (+97/‑1682) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,571,3290TG17.2% 116.3 / 28.0 64intergenic (+97/‑1682)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/35);  new base G (8/3);  total (26/38)
Fisher's exact test for biased strand distribution p-value = 3.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

TGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGA  >  minE/2571259‑2571399
                                                                       |                                                                       
tGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCAt                                                                         >  1:232100/1‑71 (MQ=255)
tGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCAt                                                                         >  1:1758303/1‑71 (MQ=255)
  cTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTa                                                                       <  1:737063/71‑1 (MQ=255)
     cTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCAtt                                                                        <  1:848762/67‑1 (MQ=255)
     cTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCAt                                                                         <  1:2139544/66‑1 (MQ=255)
        gTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaa                                                                 >  1:450382/1‑71 (MQ=255)
         tAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaa                                                                 <  1:921862/70‑1 (MQ=255)
          aaGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaat                                                                <  1:3318810/70‑1 (MQ=255)
           aGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaataa                                                              <  1:2593480/71‑1 (MQ=255)
            ggACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAg                                                             <  1:78859/71‑1 (MQ=255)
             gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                            <  1:1057921/71‑1 (MQ=255)
             gACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                            <  1:707929/71‑1 (MQ=255)
              aCTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGt                                                            <  1:707223/70‑1 (MQ=255)
                tAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaa                                                                 >  1:3075837/1‑63 (MQ=255)
                  attattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaata                                                               >  1:106744/1‑63 (MQ=255)
                  attattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                       <  1:2119564/70‑1 (MQ=255)
                   ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGt                                                                    >  1:477513/1‑57 (MQ=255)
                   ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                     <  1:1232140/71‑1 (MQ=255)
                   ttattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                     <  1:2225942/71‑1 (MQ=255)
                    tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                    >  1:418124/1‑71 (MQ=255)
                    tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                    >  1:2182597/1‑71 (MQ=255)
                    tattTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                    >  1:85200/1‑71 (MQ=255)
                     attTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGAt                                                    >  1:2254309/1‑70 (MQ=255)
                      tttttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATg                                                   <  1:3533469/70‑1 (MQ=255)
                        tttGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAg                                                 <  1:2502571/70‑1 (MQ=255)
                          tGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgtg                                               <  1:2389877/70‑1 (MQ=255)
                           gCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgtgt                                              >  1:1360047/1‑70 (MQ=255)
                             atatTACTTCATTAGCTTCGATTATTTTTCTTTAATATTCATTATGTTAATAAGtt                                                           >  1:1502246/1‑56 (MQ=255)
                             atatTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTAct                                           <  1:2694549/71‑1 (MQ=255)
                              tatTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCAt                                                                         <  1:1662302/41‑1 (MQ=255)
                               atTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaata                                                               <  1:752619/50‑1 (MQ=255)
                                ttACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGctct                                         <  1:175902/70‑1 (MQ=255)
                                  aCTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCa                                       <  1:3027968/70‑1 (MQ=255)
                                  aCTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCa                                       <  1:1985376/70‑1 (MQ=255)
                                   cTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTtaataa                                                              >  1:1194867/1‑47 (MQ=255)
                                   cTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCAtt                                     <  1:2485622/71‑1 (MQ=255)
                                      cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                     >  1:1637292/1‑52 (MQ=255)
                                      cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTg                                    <  1:2992931/69‑1 (MQ=255)
                                      cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGa                                  >  1:2008979/1‑71 (MQ=255)
                                       aTTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGGGTGCTCTCATTGGAt                                 <  1:2791507/71‑1 (MQ=255)
                                        ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAg                                                             <  1:2789876/43‑1 (MQ=255)
                                        ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                       <  1:909931/48‑1 (MQ=255)
                                        ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTg                                                       <  1:1884285/48‑1 (MQ=255)
                                          aGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGa                                                     >  1:2105731/1‑48 (MQ=255)
                                            cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                            <  1:51191/71‑1 (MQ=255)
                                            cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                            <  1:1410050/71‑1 (MQ=255)
                                            cTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtttttt                            <  1:1809107/71‑1 (MQ=255)
                                              tCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgt                                                >  1:1241951/1‑49 (MQ=255)
                                              tCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAgt                                                >  1:2625243/1‑49 (MQ=255)
                                              gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCt                                                        >  1:1733264/3‑42 (MQ=12)
                                              gtgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCTg                                                       >  1:2571275/3‑43 (MQ=16)
                                               cGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATc                         >  1:466854/1‑71 (MQ=255)
                                                gatgatTTTTCCTTTAATATCCAGCATGTAAAGAAGTTc                                                          >  1:2962167/1‑39 (MQ=9)
                                                 atgatTTTTCTTTAATATTCATTATGTTAATAAGtt                                                           <  1:1363014/36‑1 (MQ=255)
                                                 atgatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCg                       <  1:1035998/71‑1 (MQ=255)
                                                  tgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATcc                        >  1:602030/1‑71 (MQ=255)
                                                   gatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                     >  1:334079/1‑71 (MQ=255)
                                                    atTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                     <  1:833991/70‑1 (MQ=255)
                                                         tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc                     >  1:1663272/3‑67 (MQ=255)
                                                         tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                  <  1:1699436/68‑1 (MQ=255)
                                                          ccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTga                 <  1:2002593/69‑2 (MQ=255)
                                                           cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTACCCGTCTTGATc               <  1:681628/71‑1 (MQ=255)
                                                           atttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc               >  1:1204721/2‑71 (MQ=255)
                                                             ttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTg             >  1:1385251/1‑71 (MQ=255)
                                                              tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTgt            >  1:3859165/1‑70 (MQ=255)
                                                                 tatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAt                                 <  1:2954658/46‑1 (MQ=255)
                                                                 tatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTAACCGTCTTGTTCTGCCGc         <  1:1658843/70‑1 (MQ=255)
                                                                      aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt    <  1:3677991/70‑1 (MQ=255)
                                                                      aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt    <  1:2701468/70‑1 (MQ=255)
                                                                       ttATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga  <  1:2009661/71‑1 (MQ=255)
                                                                       |                                                                       
TGCTCCTTGTAAGGACTAATTATTTTTGCATATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGA  >  minE/2571259‑2571399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: